PMID- 29563964 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20220316 IS - 1750-9378 (Print) IS - 1750-9378 (Electronic) IS - 1750-9378 (Linking) VI - 13 DP - 2018 TI - Comparative assessment of HPV, alcohol and tobacco etiological fractions in Algerian patients with laryngeal squamous cell carcinoma. PG - 8 LID - 10.1186/s13027-018-0181-x [doi] LID - 8 AB - BACKGROUND: Despite the increasing incidence of laryngeal squamous cell carcinoma (LSCC) in Algeria, scarce information is available on the importance of the preventable etiological factors which may drive the disease. Remarkably, a significant number of cases occur in nonsmoker and nondrinker patients; hence, suggesting that alternative risk factors, like Human papillomavirus (HPV), might be etiologically involved. To gain more insight on the risk factors associated with the disease in the country, we evaluated the etiological fraction of HPV in comparison to tobacco and alcohol intake in LSCC patients. METHODS: To evaluate the etiopathologic fraction (EF) for HPV compared to history of tobacco and alcohol in LSCC, HPV DNA presence in 46 invasive and 3 non-invasive formalin-fixed paraffin-embedded laryngeal tumors was screened using the SPF10-DEIA-LiPA25 Assay. Demographic data and information related to exposure to the risk factors were gathered through interviewer-assisted questionnaires. RESULTS: We observed that 40.8% of all LSCC cases were associated with smoking, 40.8% had combined tobacco and alcohol exposure history, and 14.3% did not show prior exposure to either risk factor. 1 out of 3 in-situ carcinoma cases was positive for HPV-6. HPV prevalence was null in the invasive tumors. HPV DNA was detected in 2.38% for all studied cases. 10.2% of LSCC patients did not associate with any of the studied risk factors. CONCLUSION: Here we show that HPV etiological fraction in LSCC Algerian patients is low and smoking and alcohol remain the principal etiopathologic risk for LSCC burden in Algeria. FAU - Kariche, Nora AU - Kariche N AD - 1Department of Cell and Molecular Biology, Team Cytokines and Nitric oxide synthases. Faculty of Biology, University Houari Boumediene USTHB, Bab-Ezzouar, Algiers, Algeria. ISNI: 0000 0001 2293 1293. GRID: grid.420190.e FAU - Hortal, Montserrat Torres AU - Hortal MT AD - 4Infections and Cancer Unit, Cancer Epidemiology Research Program, Catalan Institute of Oncology (ICO), Barcelona, Spain. ISNI: 0000 0001 2097 8389. GRID: grid.418701.b FAU - Benyahia, Samir AU - Benyahia S AD - Oto-rhyno-laryngology Department, Mustapha Pacha Hospital, Algiers, Algeria. FAU - Alemany, Laia AU - Alemany L AD - 4Infections and Cancer Unit, Cancer Epidemiology Research Program, Catalan Institute of Oncology (ICO), Barcelona, Spain. ISNI: 0000 0001 2097 8389. GRID: grid.418701.b AD - 5Bellvitge Institute of Biomedical Research (IDIBELL), Barcelona, Spain. ISNI: 0000 0004 0427 2257. GRID: grid.418284.3 FAU - Moulai, Nabila AU - Moulai N AD - Central Laboratory for Anatomopathology, Frantz fanon Hospital, Blida, Algeria. FAU - Clavero, Omar AU - Clavero O AD - 4Infections and Cancer Unit, Cancer Epidemiology Research Program, Catalan Institute of Oncology (ICO), Barcelona, Spain. ISNI: 0000 0001 2097 8389. GRID: grid.418701.b FAU - Munoz, Marleny AU - Munoz M AD - 4Infections and Cancer Unit, Cancer Epidemiology Research Program, Catalan Institute of Oncology (ICO), Barcelona, Spain. ISNI: 0000 0001 2097 8389. GRID: grid.418701.b FAU - Ouahioune, Wahiba AU - Ouahioune W AD - Central Laboratory for Anatomopathology, Frantz fanon Hospital, Blida, Algeria. FAU - Djennaoui, Djamel AU - Djennaoui D AD - Oto-rhyno-laryngology Department, Mustapha Pacha Hospital, Algiers, Algeria. FAU - Touil-Boukoffa, Chafia AU - Touil-Boukoffa C AD - 1Department of Cell and Molecular Biology, Team Cytokines and Nitric oxide synthases. Faculty of Biology, University Houari Boumediene USTHB, Bab-Ezzouar, Algiers, Algeria. ISNI: 0000 0001 2293 1293. GRID: grid.420190.e FAU - de Sanjose, Silvia AU - de Sanjose S AD - 4Infections and Cancer Unit, Cancer Epidemiology Research Program, Catalan Institute of Oncology (ICO), Barcelona, Spain. ISNI: 0000 0001 2097 8389. GRID: grid.418701.b AD - 5Bellvitge Institute of Biomedical Research (IDIBELL), Barcelona, Spain. ISNI: 0000 0004 0427 2257. GRID: grid.418284.3 AD - CIBER in Epidemiology and Public Health (CIBERESP), Barcelona, Spain. FAU - Bourouba, Mehdi AU - Bourouba M AUID- ORCID: 0000-0002-2304-5899 AD - 1Department of Cell and Molecular Biology, Team Cytokines and Nitric oxide synthases. Faculty of Biology, University Houari Boumediene USTHB, Bab-Ezzouar, Algiers, Algeria. ISNI: 0000 0001 2293 1293. GRID: grid.420190.e LA - eng PT - Journal Article DEP - 20180313 PL - England TA - Infect Agent Cancer JT - Infectious agents and cancer JID - 101276559 PMC - PMC5851087 OTO - NOTNLM OT - Alcohol OT - HPV OT - Larynx carcinoma OT - Risk factor OT - Tobacco COIS- This research was approved by the ethics committee of the Algerian National Agency for Research and Development in Health (ATRSS). Informed consent was obtained from all individual participants included in the study.Not applicable in this section.The authors declare that they have no competing interests.Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. EDAT- 2018/03/23 06:00 MHDA- 2018/03/23 06:01 PMCR- 2018/03/13 CRDT- 2018/03/23 06:00 PHST- 2018/01/13 00:00 [received] PHST- 2018/03/06 00:00 [accepted] PHST- 2018/03/23 06:00 [entrez] PHST- 2018/03/23 06:00 [pubmed] PHST- 2018/03/23 06:01 [medline] PHST- 2018/03/13 00:00 [pmc-release] AID - 181 [pii] AID - 10.1186/s13027-018-0181-x [doi] PST - epublish SO - Infect Agent Cancer. 2018 Mar 13;13:8. doi: 10.1186/s13027-018-0181-x. eCollection 2018.