PMID- 29563985
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240314
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 15
IP  - 4
DP  - 2018 Apr
TI  - Correlation between the expression of Drp1 in vascular endothelial cells and 
      inflammatory factors in hypertension rats.
PG  - 3892-3898
LID - 10.3892/etm.2018.5899 [doi]
AB  - The objective of this study was to investigate the expression level of 
      dynamin-related protein 1 (Drp1) in vascular endothelium of hypertension rats and 
      its correlation with expression of inflammatory factors. Twenty spontaneous 
      hypertension rats (SHR) were randomly divided into SHR group (n=10) and 
      inhibition group (MD group, n=10), and the Sprague Dawley rats were enrolled as 
      the control group (C group, n=10). For rats in the MD group, Mdivi-1, a 
      mitochondrial division inhibitor, was given in dosage of 25 mg/kg. After 4 weeks 
      of administration, blood pressure was measured via tail-artery blood pressure 
      measurement. The blood samples collected from the abdominal aorta of rats were 
      used to assay the C-reaction protein (CRP) concentration in serum through 
      radioimmunoassay. Hematoxylin and eosin (H&E) staining was performed for sections 
      of thoracic aorta for morphological observation and measurement of medial 
      thickness. Enzyme-linked immunosorbant assay (ELISA), semi-quantitative real-time 
      polymerase chain reaction (RT-PCR) and western blotting was carried out for 
      detecting the expression levels of interleukin-6 (IL-6) and tumor necrosis 
      factor-alpha (TNF-alpha). Drp1 and monocyte chemotactic protein 1 (MCP-1). After 4 weeks 
      of drug administration, the blood pressure in the MD group was significantly 
      higher (P<0.01). The medial thickness of the thoracic aorta in the MD group was 
      significantly decreased in comparison with the SHR group (P<0.01). The results of 
      ELISA showed that compared with the SHR group, the expression levels of IL-6 and 
      TNF-alpha in the MD group were remarkably decreased (P<0.01). Semi-quantitative 
      RT-PCR results indicated that the mRNA expression levels of Drp1 and MCP-1 in the 
      MD group were significantly lower than those in the SHR group (P<0.05). In the 
      SHR rats, after administration of Mdivi-1, the expression of Drp1 is decreased, 
      which contributes to the alleviation in inflammatory reactions and protects the 
      vessels in SHR rats.
FAU - Liu, Xinghui
AU  - Liu X
AD  - Department of Cardiology, Guizhou Provincial People's Hospital, Guiyang, Guizhou 
      550002, P.R. China.
FAU - Tan, Hongwen
AU  - Tan H
AD  - Department of Cardiology, Guizhou Provincial People's Hospital, Guiyang, Guizhou 
      550002, P.R. China.
FAU - Liu, Xiaoqiao
AU  - Liu X
AD  - Department of Cardiology, Guizhou Provincial People's Hospital, Guiyang, Guizhou 
      550002, P.R. China.
FAU - Wu, Qiang
AU  - Wu Q
AD  - Department of Cardiology, Guizhou Provincial People's Hospital, Guiyang, Guizhou 
      550002, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20180227
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC5858055
OTO - NOTNLM
OT  - dynamin-related protein 1
OT  - inflammatory factors
OT  - spontaneous hypertension rat
OT  - vessels
EDAT- 2018/03/23 06:00
MHDA- 2018/03/23 06:01
PMCR- 2018/02/27
CRDT- 2018/03/23 06:00
PHST- 2017/11/08 00:00 [received]
PHST- 2018/01/04 00:00 [accepted]
PHST- 2018/03/23 06:00 [entrez]
PHST- 2018/03/23 06:00 [pubmed]
PHST- 2018/03/23 06:01 [medline]
PHST- 2018/02/27 00:00 [pmc-release]
AID - ETM-0-0-5899 [pii]
AID - 10.3892/etm.2018.5899 [doi]
PST - ppublish
SO  - Exp Ther Med. 2018 Apr;15(4):3892-3898. doi: 10.3892/etm.2018.5899. Epub 2018 Feb 
      27.