PMID- 29565168
OWN - NLM
STAT- MEDLINE
DCOM- 20180927
LR  - 20181023
IS  - 1699-5198 (Electronic)
IS  - 0212-1611 (Linking)
VI  - 35
IP  - 1
DP  - 2018 Jan 16
TI  - Tissular growth factors profile after teduglutide administration on an animal 
      model of intestinal anastomosis.
PG  - 185-193
LID - 10.20960/nh.1326 [doi]
AB  - BACKGROUND: Teduglutide is an enterotrophic analogue of glucagon-like peptide-2, 
      with an indirect and poorly understood mechanism of action, approved for the 
      rehabilitation of short-bowel syndrome. This study aims to analyze the response 
      of tissue growth factors to surgical injury and teduglutide administration on an 
      animal model of intestinal anastomosis. METHODS: Wistar rats (n = 59) were 
      distributed into four groups: "ileal resection" or "laparotomy", each one 
      subdivided into "postoperative teduglutide administration" or "no treatment"; and 
      sacrificed at the third or the seventh day, with ileal sample harvesting. Gene 
      expression of insulin-like growth factor 1 (Igf1), vascular endothelial growth 
      factor a (Vegfa), transforming growth factor beta1 (Tgfbeta1), connective tissue growth 
      factor (Ctgf), fibroblast growth factor 2 (Fgf2), fibroblast growth factor 7 
      (Fgf7), epidermal growth factor (Egf), heparin-binding epidermal-like growth 
      factor (Hbegf), platelet-derived growth factor b (Pdgfb) and glucagon-like 
      peptide 2 receptor (Glp2r)was studied by real-time polymerase chain reaction. 
      RESULTS: Upregulation of Fgf7, Fgf2, Egf, Vegfaand Glp2rat the third day and of 
      Pdgfat the seventh day was verified in the perianastomotic segment. Teduglutide 
      administration was associated with higher fold-change of relative gene expression 
      of Vegfa(3.6 +/- 1.3 vs.1.9 +/- 2.0, p = 0.0001), Hbegf(2.2 +/- 2.3 vs. 1.1 +/- 0.9, p = 
      0.001), Igf1(1.6 +/- 7.6 vs. 0.9 +/- 0.7, p = 0.002) and Ctgf(1.1 +/- 2.1 vs. 0.6 +/- 
      2.0, p = 0.013); and lower fold-change of Tgfbeta1, Fgf7and Glp2r. CONCLUSIONS: 
      Those results underscore the recognized role of Igf1and Hbegfas molecular 
      mediators of the effects of teduglutide and suggest that other humoral factors, 
      like Vegfand Ctgf, may also be relevant in the perioperative context. Induction 
      of Vegfa, Igf1and Ctgfgene expressions might indicate a favorable influence of 
      teduglutide on the intestinal anastomotic healing.
FAU - Costa, Beatriz Pinto
AU  - Costa BP
AD  - "A" Surgical Department, Hospitais da Universidade de Coimbra, Centro Hospitalar 
      e Universitario de Coimbra, Coimbra, Portugal; Faculty of Medicine, University of 
      Coimbra, Coimbra, Portugal. beatrizpcosta@iol.pt.
FAU - Goncalves, Ana Cristina
AU  - Goncalves AC
FAU - Abrantes, Ana Margarida
AU  - Abrantes AM
FAU - Alves, Raquel
AU  - Alves R
FAU - Matafome, Paulo
AU  - Matafome P
FAU - Seica, Raquel
AU  - Seica R
FAU - Sarmento-Ribeiro, Ana Bela
AU  - Sarmento-Ribeiro AB
FAU - Botelho, Maria Filomena
AU  - Botelho MF
FAU - Castro-Sousa, Francisco
AU  - Castro-Sousa F
LA  - eng
PT  - Journal Article
TT  - Perfil tisular de factores de crecimiento postadministracion de teduglutida en un 
      modelo animal de anastomosis intestinal.
DEP - 20180116
PL  - Spain
TA  - Nutr Hosp
JT  - Nutricion hospitalaria
JID - 9100365
RN  - 0 (Gastrointestinal Agents)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Peptides)
RN  - 7M19191IKG (teduglutide)
SB  - IM
MH  - *Anastomosis, Surgical
MH  - Animals
MH  - Gastrointestinal Agents/*pharmacology
MH  - Gene Expression Regulation/drug effects
MH  - Ileum/metabolism/surgery
MH  - Intercellular Signaling Peptides and Proteins/biosynthesis/genetics/*metabolism
MH  - Intestines/*drug effects/*surgery
MH  - Male
MH  - Peptides/*pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Short Bowel Syndrome
EDAT- 2018/03/23 06:00
MHDA- 2018/09/28 06:00
CRDT- 2018/03/23 06:00
PHST- 2017/06/03 00:00 [received]
PHST- 2017/07/21 00:00 [accepted]
PHST- 2018/03/23 06:00 [entrez]
PHST- 2018/03/23 06:00 [pubmed]
PHST- 2018/09/28 06:00 [medline]
AID - 10.20960/nh.1326 [doi]
PST - epublish
SO  - Nutr Hosp. 2018 Jan 16;35(1):185-193. doi: 10.20960/nh.1326.