PMID- 29565253
OWN - NLM
STAT- MEDLINE
DCOM- 20180723
LR  - 20180723
IS  - 0177-5103 (Print)
IS  - 0177-5103 (Linking)
VI  - 128
IP  - 1
DP  - 2018 Mar 22
TI  - Virulence marker candidates in N-protein of viral haemorrhagic septicaemia virus 
      (VHSV): virulence variability within VHSV Ib clones.
PG  - 51-62
LID - 10.3354/dao03215 [doi]
AB  - Four major genotypes of viral haemorrhagic septicaemia virus (VHSV), which have 
      been isolated from many marine and freshwater fish species, are known to differ 
      in virulence. While fast and low-cost genotyping systems based on monoclonal 
      antibodies (MAbs) have been developed for typing of VHSV virulence, there is a 
      need for supplementing the knowledge. In particular, 2 field isolates from viral 
      haemorrhagic septicaemia (VHS) outbreaks in sea-reared rainbow trout Oncorhynchus 
      mykiss in Sweden, SE-SVA-14 and SE-SVA-1033 (both genotype Ib), have yielded 
      contradictory reactions. In the present study, upon cloning by limited dilution, 
      both isolates appeared to be heterogeneous in terms of reactivity with nucleo 
      (N)-protein-specific MAbs as well their gene sequences. Infection trials in 
      rainbow trout further revealed differences in the virulence of these virus clones 
      derived from the same primary isolate. Based on a comparative analysis of the 
      entire genome of the clones tested, we suggest that the differences in virulence 
      are tentatively linked to substitutions of amino acids (aa) in the N-protein 
      region covered by aa 43-46 and aa position 168, or a combination of the two. The 
      fact that such minor naturally occurring genetic differences affect the virulence 
      implies that even low-virulent VHSV isolates in the marine environment should be 
      considered as a potential threat for the trout farming industry. The described 
      MAbs can represent useful tools for initial risk assessment of disease outbreaks 
      in farmed trout by marine VHSV isolates.
FAU - Ito, Takafumi
AU  - Ito T
AD  - Tamaki Laboratory, Research Center for Fish Diseases, National Research Institute 
      of Aquaculture, Fisheries Research Agency, 224-1 Hiruda, Tamaki, Mie 519-0423, 
      Japan.
FAU - Kurita, Jun
AU  - Kurita J
FAU - Mori, Koh-Ichiro
AU  - Mori KI
FAU - Skall, Helle Frank
AU  - Skall HF
FAU - Lorenzen, Niels
AU  - Lorenzen N
FAU - Vendramin, Niccolo
AU  - Vendramin N
FAU - Gedsted Andersen, Nikolaj
AU  - Gedsted Andersen N
FAU - Einer-Jensen, Katja
AU  - Einer-Jensen K
FAU - Olesen, Niels Jorgen
AU  - Olesen NJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Dis Aquat Organ
JT  - Diseases of aquatic organisms
JID - 8807037
RN  - 0 (Genetic Markers)
RN  - 0 (Nucleocapsid Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Fish Diseases/virology
MH  - Genetic Markers
MH  - Genotype
MH  - Hemorrhagic Septicemia, Viral/*virology
MH  - Novirhabdovirus/genetics/*metabolism/pathogenicity
MH  - Nucleocapsid Proteins/genetics/*metabolism
MH  - Oncorhynchus mykiss/virology
MH  - Phylogeny
MH  - Sweden
MH  - Virulence
OTO - NOTNLM
OT  - Genotype Ib
OT  - Nucleo-protein
OT  - Oncorhynchus mykiss
OT  - VHSV
OT  - Viral haemorrhagic septicaemia virus
OT  - Virulence
EDAT- 2018/03/23 06:00
MHDA- 2018/07/24 06:00
CRDT- 2018/03/23 06:00
PHST- 2018/03/23 06:00 [entrez]
PHST- 2018/03/23 06:00 [pubmed]
PHST- 2018/07/24 06:00 [medline]
AID - 10.3354/dao03215 [doi]
PST - ppublish
SO  - Dis Aquat Organ. 2018 Mar 22;128(1):51-62. doi: 10.3354/dao03215.