PMID- 29565514
OWN - NLM
STAT- MEDLINE
DCOM- 20191107
LR  - 20220409
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 22
IP  - 5
DP  - 2018 Mar
TI  - Correlation between Nurr1 expression and drug resistance in the brain of rats 
      with epilepsy.
PG  - 1506-1513
LID - 14500 [pii]
LID - 10.26355/eurrev_201803_14500 [doi]
AB  - OBJECTIVE: To investigate the correlation between nuclear receptor related 1 
      (Nurr1) expression and drug resistance in the brain of rats with epilepsy. 
      MATERIALS AND METHODS: A total of 60 adult male Sprague-Dawley rats were 
      selected, and the animal model of epilepsy was established by electrical 
      stimulation. These rats were randomly divided into the control group and the 
      drug-resistant group. The model of drug-resistant epilepsy was screened with 
      phenytoin (PHT) and phenobarbital (PB); the hippocampus and temporal lobe cortex 
      tissues were isolated from rats; the messenger ribonucleic acid (mRNA) and 
      protein levels of Nurr1 in the hippocampus and cortex tissues of the two groups 
      of rats were detected by reverse transcription polymerase chain reaction 
      (RT-PCR), Western blotting and immunohistochemistry, respectively. The budding 
      tissue marker growth-associated protein-43 (GAP43) in hippocampus tissues were 
      labeled by immunofluorescence staining; the mRNA and protein levels of indicators 
      related to drug-resistant epilepsy, including leukemia-associated phenotype 
      (LAP), multi-drug resistance protein (MRP), P-glycoprotein (P-gp) and monocyte 
      chemoattractant protein-1 (MCP-1), were further detected. Pearson correlation 
      analysis was used to analyze the correlations of the protein level of Nurr1 with 
      drug-resistant indicators. RESULTS: The mRNA and protein levels of Nurr1 in 
      hippocampus tissues of the drug-resistant group were significantly increased 
      compared with those of the control group (p < 0.05). In temporal lobe cortex 
      tissues, there was no significant difference in the mRNA level of Nurr1 between 
      the control group and the drug-resistant group (p > 0.05). The 
      immunohistochemistry results showed that the fluorescence intensity of GAP3 in 
      hippocampus tissues of the drug-resistant group was significantly higher than 
      that of the control group. The mRNA and protein levels of epilepsy-related 
      indicators, LAP, MRP, P-gp and MCP-1 in hippocampus tissues of drug-resistant 
      group were significantly up-regulated compared with those of the control group (p 
      < 0.05). Pearson correlation analysis indicated that the protein level of Nurr1 
      in hippocampus tissues was positively correlated with those of LAP, MRP, P-gp and 
      MCP-1 in the corresponding regions (p < 0.05). CONCLUSIONS: Nurr1 enhances the 
      drug resistance of epilepsy in rats by up-regulating the expression of proteins 
      related to drug resistance.
FAU - Li, H-Y
AU  - Li HY
AD  - Department of Emergency, The affiliated Yantai Yuhuangding Hospital of Qingdao 
      University, Yantai, China. 1136042700@qq.com.
FAU - Liu, F
AU  - Liu F
FAU - Wang, H-R
AU  - Wang HR
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (Nr4a2 protein, rat)
RN  - 0 (Nuclear Receptor Subfamily 4, Group A, Member 2)
SB  - IM
MH  - Animals
MH  - Disease Models, Animal
MH  - Drug Resistance/genetics
MH  - Epilepsy/*drug therapy/metabolism
MH  - Hippocampus/metabolism
MH  - Male
MH  - Nuclear Receptor Subfamily 4, Group A, Member 2/analysis/genetics/*physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2018/03/23 06:00
MHDA- 2019/11/08 06:00
CRDT- 2018/03/23 06:00
PHST- 2018/03/23 06:00 [entrez]
PHST- 2018/03/23 06:00 [pubmed]
PHST- 2019/11/08 06:00 [medline]
AID - 14500 [pii]
AID - 10.26355/eurrev_201803_14500 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2018 Mar;22(5):1506-1513. doi: 
      10.26355/eurrev_201803_14500.