PMID- 29565566
OWN - NLM
STAT- MEDLINE
DCOM- 20190312
LR  - 20190312
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 90
IP  - 8
DP  - 2018 Apr 17
TI  - One-Shot Dual-Code Immunotargeting for Ultra-Sensitive Tumor Necrosis Factor-alpha 
      Nanosensors by 3D Enhanced Dark-Field Super-Resolution Microscopy.
PG  - 5100-5107
LID - 10.1021/acs.analchem.7b05107 [doi]
AB  - Tumor necrosis factor-alpha (TNF-alpha) is a significant mediator of autoimmune diseases 
      and an inflammatory protein biomarker. A novel method for the immunotargeting of 
      TNF-alpha has been developed using three-dimensional (3D) enhanced dark-field 
      super-resolution microscopy (3D EDF-SRM) based on ultrasensitive dual-code 
      plasmonic nanosensing. Dual-code EDF-based 3D SRM improved the localization 
      precision and sensitivity with a least-cubic algorithm, which provides accurate 
      position information for the immunotargeted site. A dual-view device and digital 
      single-lens reflex (DSLR) camera were used for simultaneous dual confirmable 
      quantitative and qualitative immunoscreening based on enhanced dark-field 
      scattering images. Two different sizes of silver nanoparticles (40- and 80-nm 
      AgNPs) were compared to enhance the scattering signal of the immunotargeted 
      plasmonic nanoprobe for the 3D EDF-SRM system. The standard TNF-alpha was 
      immunotargeted at a single-molecule level and was quantitatively analyzed by 
      measuring the scattering signals of 80 nm AgNPs on an array chip with 
      gold-nanostages (GNSs) with 100 nm spot diameters. The localization precision in 
      the 80 nm AgNP immunotag on the GNS narrowed to  approximately 9.5 nm after applying the 
      least-cubic algorithm. The developed nanosensor exhibited a detection limit of 65 
      zM (1.14 ag/mL; S/N = 3) with a wide dynamic detection range of 65 zM-2.08 pM 
      (1.14 ag/mL-36.4 pg/mL; R = 0.9921). These values are 20-33 400 000 times lower 
      than detection limits obtained using previous methods. In addition, a recovery 
      greater than 98% was achieved by spiking standard TNF-alpha into human serum samples. 
      This method should facilitate simultaneous improvements in immunotargeting 
      precision and ultrahigh sensitive detection of various disease-related target 
      protein molecules at a single-molecule level.
FAU - Ju, Soyeong
AU  - Ju S
AD  - Department of Chemistry, Graduate School , Kyung Hee University , Yongin-si , 
      Gyeonggi-do 17104 , Republic of Korea.
FAU - Lee, Seungah
AU  - Lee S
AD  - Department of Applied Chemistry and Institute of Natural Sciences , Kyung Hee 
      University , Yongin-si , Gyeonggi-do 17104 , Republic of Korea.
FAU - Chakkarapani, Suresh Kumar
AU  - Chakkarapani SK
AD  - Department of Chemistry, Graduate School , Kyung Hee University , Yongin-si , 
      Gyeonggi-do 17104 , Republic of Korea.
FAU - Kim, Kyungsoo
AU  - Kim K
AD  - Department of Applied Mathematics , Kyung Hee University , Yongin-si , 
      Gyeonggi-do 17104 , Republic of Korea.
FAU - Yu, Hyunung
AU  - Yu H
AD  - Nanobio Fusion Research Center , Korea Research Institute of Standards and 
      Science , Daejeon 34113 , Republic of Korea.
FAU - Kang, Seong Ho
AU  - Kang SH
AUID- ORCID: 0000-0003-2101-4113
AD  - Department of Chemistry, Graduate School , Kyung Hee University , Yongin-si , 
      Gyeonggi-do 17104 , Republic of Korea.
AD  - Department of Applied Chemistry and Institute of Natural Sciences , Kyung Hee 
      University , Yongin-si , Gyeonggi-do 17104 , Republic of Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180330
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Antibodies, Immobilized)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 3M4G523W1G (Silver)
RN  - 7440-57-5 (Gold)
SB  - IM
MH  - Antibodies, Immobilized/chemistry/immunology
MH  - Gold/chemistry
MH  - Humans
MH  - Limit of Detection
MH  - Metal Nanoparticles/chemistry
MH  - Microscopy/*methods
MH  - Nanotechnology
MH  - Silver/chemistry
MH  - Tumor Necrosis Factor-alpha/*analysis/blood/immunology
EDAT- 2018/03/23 06:00
MHDA- 2019/03/13 06:00
CRDT- 2018/03/23 06:00
PHST- 2018/03/23 06:00 [pubmed]
PHST- 2019/03/13 06:00 [medline]
PHST- 2018/03/23 06:00 [entrez]
AID - 10.1021/acs.analchem.7b05107 [doi]
PST - ppublish
SO  - Anal Chem. 2018 Apr 17;90(8):5100-5107. doi: 10.1021/acs.analchem.7b05107. Epub 
      2018 Mar 30.