PMID- 29568344
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 1949-2553 (Electronic)
IS  - 1949-2553 (Linking)
VI  - 9
IP  - 17
DP  - 2018 Mar 2
TI  - Correlation and agreement between eplet mismatches calculated using serological, 
      low-intermediate and high resolution molecular human leukocyte antigen typing 
      methods.
PG  - 13116-13124
LID - 10.18632/oncotarget.24349 [doi]
AB  - Structural human leukocyte antigen (HLA) matching at the eplet level can be 
      identified by HLAMatchmaker, which requires the entry of four-digit alleles. The 
      aim of this study was to evaluate the agreement between eplet mismatches 
      calculated by serological and two-digit typing methods compared to 
      high-resolution four-digit typing. In a cohort of 264 donor/recipient pairs, the 
      evaluation of measurement error was assessed using intra-class correlation to 
      confirm the absolute agreement between the number of eplet mismatches at class I 
      (HLA-A, -B, C) and II loci (HLA-DQ and -DR) calculated using serological or 
      two-digit molecular typing compared to four-digit molecular typing methods. The 
      proportion of donor/recipient pairs with a difference of >5 eplet mismatches 
      between the HLA typing methods was also determined. Intra-class correlation 
      coefficients between serological and four-digit molecular typing methods were 
      0.969 (95% confidence intervals [95% CI] 0.960-0.975) and 0.926 (95% CI 
      0.899-0.944), respectively; and 0.995 (95% CI 0.994-0.996) and 0.993 (95% CI 
      0.991-0.995), respectively between two-digit and four-digit molecular typing 
      methods. The proportion of donor/recipient pairs with a difference of >5 eplet 
      mismatches at class I and II loci was 4% and 16% for serological versus 
      four-digit molecular typing methods, and 0% and 2% for two-digit versus 
      four-digit molecular typing methods, respectively. In this small predominantly 
      Caucasian population, compared with serology, there is a high level of agreement 
      in the number of eplet mismatches calculated using two-compared to four-digit 
      molecular HLA-typing methods, suggesting that two-digit typing may be sufficient 
      in determining eplet mismatch load in kidney transplantation.
FAU - Fidler, Samantha
AU  - Fidler S
AD  - Department of Clinical Immunology, Fiona Stanley Hospital, Perth, Australia.
AD  - School of Pathology and Laboratory Medicine, University of Western Australia, 
      Perth, Australia.
FAU - D'Orsogna, Lloyd
AU  - D'Orsogna L
AD  - Department of Clinical Immunology, Fiona Stanley Hospital, Perth, Australia.
AD  - School of Pathology and Laboratory Medicine, University of Western Australia, 
      Perth, Australia.
FAU - Irish, Ashley B
AU  - Irish AB
AD  - Department of Nephrology and Transplantation, Fiona Stanley Hospital, Perth, 
      Australia.
AD  - School of Medicine and Pharmacology, University of Western Australia, Perth, 
      Australia.
FAU - Lewis, Joshua R
AU  - Lewis JR
AD  - School of Medicine and Pharmacology, University of Western Australia, Perth, 
      Australia.
AD  - Centre for Kidney Research, The Children's Hospital at Westmead, Sydney, 
      Australia.
AD  - Sydney School of Public Health, University of Sydney, Sydney, Australia.
FAU - Wong, Germaine
AU  - Wong G
AD  - Centre for Kidney Research, The Children's Hospital at Westmead, Sydney, 
      Australia.
AD  - Sydney School of Public Health, University of Sydney, Sydney, Australia.
AD  - Centre for Transplant and Renal Research, Westmead Hospital, Sydney, Australia.
FAU - Lim, Wai H
AU  - Lim WH
AD  - School of Medicine and Pharmacology, University of Western Australia, Perth, 
      Australia.
AD  - Department of Renal Medicine, Sir Charles Gairdner Hospital, Perth, Australia.
LA  - eng
PT  - Journal Article
DEP - 20180201
PL  - United States
TA  - Oncotarget
JT  - Oncotarget
JID - 101532965
PMC - PMC5862565
OTO - NOTNLM
OT  - HLA typing
OT  - Immunology section
OT  - agreement
OT  - molecular
OT  - serological
COIS- CONFLICTS OF INTEREST The authors declare no conflicts of interest.
EDAT- 2018/03/24 06:00
MHDA- 2018/03/24 06:01
PMCR- 2018/03/02
CRDT- 2018/03/24 06:00
PHST- 2017/10/16 00:00 [received]
PHST- 2018/01/24 00:00 [accepted]
PHST- 2018/03/24 06:00 [entrez]
PHST- 2018/03/24 06:00 [pubmed]
PHST- 2018/03/24 06:01 [medline]
PHST- 2018/03/02 00:00 [pmc-release]
AID - 24349 [pii]
AID - 10.18632/oncotarget.24349 [doi]
PST - epublish
SO  - Oncotarget. 2018 Feb 1;9(17):13116-13124. doi: 10.18632/oncotarget.24349. 
      eCollection 2018 Mar 2.