PMID- 29568797 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20240314 IS - 2352-3859 (Print) IS - 2352-3859 (Electronic) VI - 11 DP - 2018 Mar TI - Effects of consumption of whole grape powder on basal NF-kappaB signaling and inflammatory cytokine secretion in a mouse model of inflammation. PG - 1-8 LID - 10.1016/j.jnim.2017.11.002 [doi] AB - Dietary consumption of polyphenol-rich fruits, such as grapes, may reduce inflammation and potentially prevent diseases linked to inflammation. Here, we used a genetically engineered murine model to measure Nuclear Factor kappa-light-chain-enhancer of activated B cells (NF-kappaB) activity and pro-inflammatory cytokine secretion to test the hypothesis that oral consumption of whole grape formulation reduces inflammatory signaling in the body. NF-kappaB luciferase reporter mice were divided into two groups, one which was fed an experimental diet formulated with 4% (w/w) whole grape powder (WGP) or another which was fed a control diet formulated with 3.6% glucose/fructose (w/w) combination. Simulated inflammation was induced in the mice by intraperitoneal injection of lipopolysaccharide (LPS). In vivo imaging was used to determine the effect of each diet on NF-kappaB activity. We found that there were no significant differences in weight gain between the WGP and control diet groups. However, there was a statistically significant (p<0.0001) difference in the progression of basal levels of NF-kappaB signaling between mice fed on control or WGP diet. There were no significant differences in NF-kappaB reporter indices between WGP- and control-diet groups after either acute or repeated inflammatory challenge. However, terminal blood collection revealed significantly (p<0.01) lower serum concentrations of the inflammatory cytokines Interleukin-6 (IL-6) and Tumor Necrosis Factor alpha (TNFalpha) only among WGP diet mice subjected to acute inflammatory challenge. Overall, these data suggest that while diets supplemented with WGP may suppress steady-state low levels of inflammatory signaling, such a supplementation may not alleviate exogenously induced massive NF-kappaB activation. FAU - Miller, Sonni-Ali AU - Miller SA AD - Department of Pathobiology, Tuskegee University, College of Veterinary Medicine, 1200 W. Montgomery Road, Tuskegee AL, 36088. FAU - White, Jason A AU - White JA AD - Department of Pathobiology, Tuskegee University, College of Veterinary Medicine, 1200 W. Montgomery Road, Tuskegee AL, 36088. FAU - Chowdhury, Rupak AU - Chowdhury R AD - Department of Pathobiology, Tuskegee University, College of Veterinary Medicine, 1200 W. Montgomery Road, Tuskegee AL, 36088. FAU - Gales, Dominique N AU - Gales DN AD - Department of Pathobiology, Tuskegee University, College of Veterinary Medicine, 1200 W. Montgomery Road, Tuskegee AL, 36088. FAU - Tameru, Berhanu AU - Tameru B AD - Department of Pathobiology, Tuskegee University, College of Veterinary Medicine, 1200 W. Montgomery Road, Tuskegee AL, 36088. FAU - Tiwari, Amit K AU - Tiwari AK AD - Department of Pathobiology, Tuskegee University, College of Veterinary Medicine, 1200 W. Montgomery Road, Tuskegee AL, 36088. FAU - Samuel, Temesgen AU - Samuel T AD - Department of Pathobiology, Tuskegee University, College of Veterinary Medicine, 1200 W. Montgomery Road, Tuskegee AL, 36088. LA - eng GR - G12 MD007585/MD/NIMHD NIH HHS/United States GR - SC3 GM109314/GM/NIGMS NIH HHS/United States PT - Journal Article DEP - 20171122 PL - Netherlands TA - J Nutr Intermed Metab JT - Journal of nutrition & intermediary metabolism JID - 101682791 PMC - PMC5858739 MID - NIHMS925473 OTO - NOTNLM OT - NF-kB OT - Whole grape powder OT - diet OT - in vivo OT - inflammation OT - mouse EDAT- 2018/03/24 06:00 MHDA- 2018/03/24 06:01 PMCR- 2019/03/01 CRDT- 2018/03/24 06:00 PHST- 2018/03/24 06:00 [entrez] PHST- 2018/03/24 06:00 [pubmed] PHST- 2018/03/24 06:01 [medline] PHST- 2019/03/01 00:00 [pmc-release] AID - 10.1016/j.jnim.2017.11.002 [doi] PST - ppublish SO - J Nutr Intermed Metab. 2018 Mar;11:1-8. doi: 10.1016/j.jnim.2017.11.002. Epub 2017 Nov 22.