PMID- 29568892
OWN - NLM
STAT- MEDLINE
DCOM- 20180910
LR  - 20220216
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Print)
IS  - 1107-3756 (Linking)
VI  - 41
IP  - 6
DP  - 2018 Jun
TI  - Effect of electro-acupuncture on the BDNF-TrkB pathway in the spinal cord of CCI 
      rats.
PG  - 3307-3315
LID - 10.3892/ijmm.2018.3563 [doi]
AB  - Microglia, which comprise a sensor for pathological events in the central nervous 
      system, may be triggered by nerve injury and transformed from a quiescent state 
      into an activated state; ionised calcium binding adaptor molecule 1 (Iba1) is a 
      sensitive marker associated with activated microglia. Accumulated evidence 
      suggests that spinal activated microglia and the brain-derived neurotrophic 
      factor (BDNF)-tyrosine kinase receptor B (TrkB) signalling pathway play major 
      roles in the production and development of neuropathic pain. 
      Electro-acupuncture (EA) has a positive effect on relieving chronic neuropathic 
      pain; however, the underlying mechanisms remain unclear. To determine the 
      significance of EA in the treatment of neuropathic pain mediated by activated 
      microglia and the BDNF-TrkB signalling pathway in the spinal cord, the mechanical 
      withdrawal threshold (MWT) and thermal withdrawal latency (TWL) values were 
      recorded to assess hyperalgesia and allodynia. In addition, the amount of 
      activated microglia and BDNF were assessed via immunofluorescence. Iba1, BDNF and 
      TrkB mRNA expression levels were examined using qPCR; the protein levels of BDNF, 
      p-TrkB and TrkB in the spinal cord were analysed via western blotting. The 
      present study demonstrated that EA treatment increased the MWT and TWL values. EA 
      significantly inhibited the proportion of activated microglia and BDNF expression 
      in the spinal cord after chronic constrictive injury (CCI). Furthermore, EA 
      decreased the expression of BDNF and TrkB at both the mRNA and protein levels in 
      the spinal cord of CCI rats. These findings suggest that the analgesic effect of 
      EA may be achieved by inhibiting the activation of spinal microglia and 
      subsequently blocking the BDNF-TrkB signalling pathway.
FAU - Tu, Wen-Zhan
AU  - Tu WZ
AD  - Shandong University, Jinan, Shandong 250100, P.R. China
AD  - Department of Physical Medicine and Rehabilitation, The Second Affiliated 
      Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, 
      Zhejiang 325027, P.R. China
AD  - Integrative & Optimized Medicine Research Center, China-USA Institute for 
      Acupuncture and Rehabilitation of Wenzhou Medical University, Wenzhou, Zhejiang 
      325027, P.R. China
FAU - Li, Si-Si
AU  - Li SS
AD  - Department of Physical Medicine and Rehabilitation, The Second Affiliated 
      Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, 
      Zhejiang 325027, P.R. China
FAU - Jiang, Xia
AU  - Jiang X
AD  - Department of Physical Medicine and Rehabilitation, The Second Affiliated 
      Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, 
      Zhejiang 325027, P.R. China
FAU - Qian, Xin-Ru
AU  - Qian XR
AD  - Department of Rehabilitation, NYU Langone Medical Center, New York, NY 10016, USA
AD  - Integrative & Optimized Medicine Research Center, China-USA Institute for 
      Acupuncture and Rehabilitation of Wenzhou Medical University, Wenzhou, Zhejiang 
      325027, P.R. China
FAU - Yang, Guan-Hu
AU  - Yang GH
AD  - Department of Specialty Medicine, Ohio University, Athens, OH 45701, USA
AD  - Integrative & Optimized Medicine Research Center, China-USA Institute for 
      Acupuncture and Rehabilitation of Wenzhou Medical University, Wenzhou, Zhejiang 
      325027, P.R. China
FAU - Gu, Peng-Peng
AU  - Gu PP
AD  - Department of Physical Medicine and Rehabilitation, The Second Affiliated 
      Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, 
      Zhejiang 325027, P.R. China
FAU - Lu, Bin
AU  - Lu B
AD  - Protein Quality Control and Diseases Laboratory, Attardi Institute of 
      Mitochondrial Biomedicine, School of Life Sciences, Wenzhou Medical University, 
      Wenzhou, Zhejiang 325035, P.R. China
FAU - Jiang, Song-He
AU  - Jiang SH
AD  - Department of Physical Medicine and Rehabilitation, The Second Affiliated 
      Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, 
      Zhejiang 325027, P.R. China
AD  - Integrative & Optimized Medicine Research Center, China-USA Institute for 
      Acupuncture and Rehabilitation of Wenzhou Medical University, Wenzhou, Zhejiang 
      325027, P.R. China
LA  - eng
PT  - Journal Article
DEP - 20180313
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Electroacupuncture/*methods
MH  - Male
MH  - Rats
MH  - Receptor, trkB/*metabolism
MH  - Signal Transduction/physiology
MH  - Spinal Cord/*metabolism
PMC - PMC5881805
EDAT- 2018/03/24 06:00
MHDA- 2018/09/11 06:00
PMCR- 2018/03/13
CRDT- 2018/03/24 06:00
PHST- 2016/08/08 00:00 [received]
PHST- 2018/01/23 00:00 [accepted]
PHST- 2018/03/24 06:00 [pubmed]
PHST- 2018/09/11 06:00 [medline]
PHST- 2018/03/24 06:00 [entrez]
PHST- 2018/03/13 00:00 [pmc-release]
AID - ijmm-41-06-3307 [pii]
AID - 10.3892/ijmm.2018.3563 [doi]
PST - ppublish
SO  - Int J Mol Med. 2018 Jun;41(6):3307-3315. doi: 10.3892/ijmm.2018.3563. Epub 2018 
      Mar 13.