PMID- 29569515
OWN - NLM
STAT- MEDLINE
DCOM- 20200327
LR  - 20200401
IS  - 1477-0970 (Electronic)
IS  - 1352-4585 (Print)
IS  - 1352-4585 (Linking)
VI  - 25
IP  - 5
DP  - 2019 Apr
TI  - Prevalence of salivary human herpesviruses in pediatric multiple sclerosis cases 
      and controls.
PG  - 644-652
LID - 10.1177/1352458518765654 [doi]
AB  - BACKGROUND: Multiple sclerosis (MS) is a multifactorial disease of unknown 
      origin. The current paradigm is that disease develops in genetically susceptible 
      individuals, influenced by environmental factors. Epstein-Barr virus (EBV) and 
      human herpesvirus 6 (HHV-6) have particularly strong associations with the 
      disease. Both viruses are typically acquired during childhood, decades before MS 
      presents. However, in patients with pediatric MS, the temporal window between 
      viral acquisition and disease onset is shortened, which may provide insights into 
      the association of herpesviruses with MS. OBJECTIVE: To compare the frequency of 
      EBV and HHV-6 in the saliva of a cohort of pediatric MS patients and age-matched 
      controls. METHODS: The study enrolled 32 pediatric MS patients and 42 controls 
      and evaluated saliva for HHV-6 u57 and EBV lmp-1 amplification by droplet digital 
      polymerase chain reaction (ddPCR). RESULTS: Pediatric MS patients did not differ 
      from controls in the frequency or magnitude of salivary viral shedding. During 
      the assessment of EBV positivity, distinct profiles emerged that correlated with 
      target amplicon mutations. CONCLUSIONS: None of these mutations were evident in 
      EBV-positive samples from pediatric MS patients, whereas they were present in 
      pediatric controls, in addition to MS and control adults, suggesting differential 
      host-immune control of EBV in this pediatric MS cohort.
FAU - Leibovitch, Emily C
AU  - Leibovitch EC
AD  - National Institutes of Health, National Institute of Neurological Disorders and 
      Stroke, Bethesda, MD, USA.
FAU - Lin, Cheng-Te Major
AU  - Lin CM
AD  - National Institutes of Health, National Institute of Neurological Disorders and 
      Stroke, Bethesda, MD, USA.
FAU - Billioux, Bridgette J
AU  - Billioux BJ
AD  - National Institutes of Health, National Institute of Neurological Disorders and 
      Stroke, Bethesda, MD, USA.
FAU - Graves, Jennifer
AU  - Graves J
AD  - Multiple Sclerosis Center, University of California San Francisco, San Francisco, 
      CA, USA.
FAU - Waubant, Emmanuelle
AU  - Waubant E
AD  - Multiple Sclerosis Center, University of California San Francisco, San Francisco, 
      CA, USA.
FAU - Jacobson, Steven
AU  - Jacobson S
AD  - National Institutes of Health, National Institute of Neurological Disorders and 
      Stroke, Bethesda, MD, USA.
LA  - eng
GR  - Z01 NS002817-18/Intramural NIH HHS/United States
GR  - Z01 NS002817-19/Intramural NIH HHS/United States
PT  - Journal Article
DEP - 20180323
PL  - England
TA  - Mult Scler
JT  - Multiple sclerosis (Houndmills, Basingstoke, England)
JID - 9509185
SB  - IM
MH  - Adult
MH  - Child
MH  - Cohort Studies
MH  - Epstein-Barr Virus Infections/*epidemiology
MH  - Female
MH  - Herpesviridae/*pathogenicity
MH  - Herpesvirus 4, Human/immunology/pathogenicity
MH  - Humans
MH  - Male
MH  - Multiple Sclerosis/*epidemiology/*virology
MH  - Prevalence
MH  - Saliva/*virology
MH  - Virus Shedding/immunology
PMC - PMC6119543
MID - NIHMS946930
OTO - NOTNLM
OT  - Epstein-Barr virus
OT  - Multiple sclerosis
OT  - ddPCR
OT  - herpesvirus
OT  - human herpesvirus 6
OT  - pediatric multiple sclerosis
OT  - saliva
EDAT- 2018/03/24 06:00
MHDA- 2020/03/28 06:00
PMCR- 2020/04/01
CRDT- 2018/03/24 06:00
PHST- 2018/03/24 06:00 [pubmed]
PHST- 2020/03/28 06:00 [medline]
PHST- 2018/03/24 06:00 [entrez]
PHST- 2020/04/01 00:00 [pmc-release]
AID - 10.1177/1352458518765654 [doi]
PST - ppublish
SO  - Mult Scler. 2019 Apr;25(5):644-652. doi: 10.1177/1352458518765654. Epub 2018 Mar 
      23.