PMID- 29569871
OWN - NLM
STAT- MEDLINE
DCOM- 20190208
LR  - 20190215
IS  - 2038-8306 (Electronic)
IS  - 1121-760X (Print)
IS  - 1121-760X (Linking)
VI  - 62
IP  - 1
DP  - 2018 Jan 22
TI  - LOX-1 deficient mice show resistance to zymosan-induced arthritis.
PG  - 2847
LID - 10.4081/ejh.2018.2847 [doi]
LID - 2847
AB  - Recent data suggest that the lectin-like oxidized low-density lipoprotein 
      (ox-LDL) receptor-1 (LOX-1)/ox-LDL system may be involved in the pathogenesis of 
      arthritis. We aimed to demonstrate the roles of the LOX-1/ox-LDL system in 
      arthritis development by using LOX-1 knockout (KO) mice. Arthritis was induced in 
      the right knees of C57Bl/6 wild-type (WT) and LOX-1 KO mice via zymosan 
      injection. Saline was injected in the left knees. Arthritis development was 
      evaluated using inflammatory cell infiltration, synovial hyperplasia, and 
      cartilage degeneration scores at 1, 3, and 7 days after administration. LOX-1, 
      ox-LDL, and matrix metalloproteinase-3 (MMP-3) expression in the synovial cells 
      and chondrocytes was evaluated by immunohistochemistry. The LOX-1, ox-LDL, and 
      MMP-3 expression levels in synovial cells were scored on a grading scale. The 
      positive cell rate of LOX-1, ox-LDL, and MMP-3 in chondrocytes was measured. The 
      correlation between the positive cell rate of LOX-1 or ox-LDL and the cartilage 
      degeneration score was also examined. Inflammatory cell infiltration, synovial 
      hyperplasia, and cartilage degeneration were significantly reduced in the LOX-1 
      KOmice with zymosan-induced arthritis (ZIA) compared to WT mice with ZIA. In the 
      saline-injected knees, no apparent arthritic changes were observed. LOX-1 and 
      ox-LDL expression in synovial cells and chondrocytes were detected in the knees 
      of WT mice with ZIA. No LOX-1 and ox-LDL expression was detected in the knees of 
      LOX-1 KOmice with ZIA or the saline-injected knees of both mice. MMP-3 expression 
      in the synovial cells and chondrocytes was also detected in knees of both mice 
      with ZIA, and was significantly less in the LOX-1 KO mice than in WT mice. The 
      positive cell rate of LOX-1 or ox-LDL and the cartilage degeneration score showed 
      a positive correlation. Our data show the involvement of the LOX-1/ox-LDL system 
      in murine ZIA development. LOX-1-positive synovial cells and chondrocytes are 
      potential therapeutic targets for arthritis prevention.
FAU - Hashimoto, Kazuhiko
AU  - Hashimoto K
AD  - Kindai University Hospital. hazzhiko@med.kindai.ac.jp.
FAU - Oda, Yutaka
AU  - Oda Y
FAU - Nakagawa, Koichi
AU  - Nakagawa K
FAU - Ikeda, Terumasa
AU  - Ikeda T
FAU - Ohtani, Kazuhiro
AU  - Ohtani K
FAU - Akagi, Masao
AU  - Akagi M
LA  - eng
PT  - Journal Article
DEP - 20180122
PL  - Italy
TA  - Eur J Histochem
JT  - European journal of histochemistry : EJH
JID - 9207930
RN  - 0 (OLR1 protein, human)
RN  - 0 (Scavenger Receptors, Class E)
RN  - 9010-72-4 (Zymosan)
SB  - IM
MH  - Animals
MH  - Arthritis/*chemically induced/physiopathology
MH  - Disease Models, Animal
MH  - Disease Resistance/genetics
MH  - Gene Deletion
MH  - Gene Knockout Techniques
MH  - Immunohistochemistry
MH  - Knee Joint/pathology
MH  - Mice
MH  - *Scavenger Receptors, Class E/genetics
MH  - Synovial Fluid/cytology
MH  - Zymosan/*toxicity
PMC - PMC5806501
OTO - NOTNLM
OT  - Arthritis
OT  - Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1)
OT  - oxidized low-density lipoprotein (ox-LDL)
COIS- Conflict of interest: The authors declare no conflict of interest.
EDAT- 2018/03/24 06:00
MHDA- 2019/02/09 06:00
PMCR- 2018/01/22
CRDT- 2018/03/24 06:00
PHST- 2017/09/02 00:00 [received]
PHST- 2018/01/08 00:00 [accepted]
PHST- 2018/01/03 00:00 [revised]
PHST- 2018/03/24 06:00 [entrez]
PHST- 2018/03/24 06:00 [pubmed]
PHST- 2019/02/09 06:00 [medline]
PHST- 2018/01/22 00:00 [pmc-release]
AID - 10.4081/ejh.2018.2847 [doi]
PST - epublish
SO  - Eur J Histochem. 2018 Jan 22;62(1):2847. doi: 10.4081/ejh.2018.2847.