PMID- 29572861
OWN - NLM
STAT- MEDLINE
DCOM- 20190208
LR  - 20200310
IS  - 1531-4995 (Electronic)
IS  - 0023-852X (Linking)
VI  - 128
IP  - 9
DP  - 2018 Sep
TI  - Expression of tenascin-C in a rat vocal fold injury model and its regulation of 
      fibroblasts.
PG  - E316-E322
LID - 10.1002/lary.27164 [doi]
AB  - OBJECTIVES/HYPOTHESIS: Tenascin-C (Tnc) is an extracellular matrix (ECM) 
      glycoprotein that plays a vital role in wound healing and fibrotic disease. Tnc 
      is highly upregulated soon after vocal fold injury, but its function in the vocal 
      fold has not yet been defined. In this study, we investigated Tnc expression in a 
      rat vocal fold injury model in vivo and its roles in fibroblasts in vitro. STUDY 
      DESIGN: In vivo and in vitro. METHODS: Tnc mRNA and protein expression levels 
      were quantified on days 3, 7, 14, 28, and 56 after vocal fold injury in 
      Sprague-Dawley rats. In vitro, immunocytochemistry, Western blot, and 
      quantitative real-time polymerase chain reaction (qRT-PCR) analyses were 
      performed in primary rat vocal fold fibroblasts following Tnc or transforming 
      growth factor (TGF)-beta1 stimulation to investigate the phenotypic effects. 
      RESULTS: Tnc mRNA and protein expression was upregulated dramatically on days 3 
      and 7 after injury, and significant differences were observed by qRT-PCR 
      (P < .01). In vitro, Tnc significantly stimulated the migration of primary vocal 
      fold fibroblasts. Following incubation with Tnc for 72 hours, alpha-smooth muscle 
      actin, collagen I, and fibronectin expression was significantly upregulated 
      (P < .05). TGF-beta1 increased Tnc expression in a time-dependent manner, and a 
      mothers against decapentaplegic homolog 3 (SMAD3) inhibitor attenuated this 
      TGF-beta1-induced stimulation. CONCLUSIONS: Tnc was highly upregulated during the 
      early postinjury period in vivo and promoted vocal fold fibroblast migration, 
      transdifferentiation, and ECM protein synthesis in vitro. Tnc was induced by 
      TGF-beta1 in a SMAD3-dependent manner. Transient expression of Tnc is likely to 
      promote regeneration, but its potential role in fibrosis requires further study. 
      LEVEL OF EVIDENCE: NA Laryngoscope, 128:E316-E322, 2018.
CI  - (c) 2018 The American Laryngological, Rhinological and Otological Society, Inc.
FAU - Li, Juan
AU  - Li J
AUID- ORCID: 0000-0001-6578-4764
AD  - Department of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, 
      Capital Medical University, Key Laboratory of Otolaryngology-Head and Neck 
      Surgery, Ministry of Education of China, Beijing, China.
FAU - Liu, Yiqiong
AU  - Liu Y
AD  - State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life 
      Sciences, PKU-IDG/McGovern Institute for Brain Research, Peking University, 
      Beijing, China.
FAU - Wang, Yiming
AU  - Wang Y
AD  - State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life 
      Sciences, PKU-IDG/McGovern Institute for Brain Research, Peking University, 
      Beijing, China.
FAU - Xu, Wen
AU  - Xu W
AD  - Department of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, 
      Capital Medical University, Key Laboratory of Otolaryngology-Head and Neck 
      Surgery, Ministry of Education of China, Beijing, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180323
PL  - United States
TA  - Laryngoscope
JT  - The Laryngoscope
JID - 8607378
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Smad3 Protein)
RN  - 0 (Smad3 protein, rat)
RN  - 0 (Tenascin)
RN  - 0 (Tgfb1 protein, rat)
RN  - 0 (Transforming Growth Factor beta1)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/physiology
MH  - Cell Movement/physiology
MH  - Disease Models, Animal
MH  - Extracellular Matrix Proteins/metabolism
MH  - Fibroblasts/*metabolism
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Smad3 Protein/metabolism
MH  - Tenascin/*metabolism
MH  - Transforming Growth Factor beta1/metabolism
MH  - Up-Regulation
MH  - Vocal Cords/*injuries/metabolism
OTO - NOTNLM
OT  - Tenascin-C
OT  - extracellular matrix protein
OT  - transforming growth factor-beta
OT  - vocal fold
OT  - wound healing
EDAT- 2018/03/25 06:00
MHDA- 2019/02/09 06:00
CRDT- 2018/03/25 06:00
PHST- 2017/12/04 00:00 [received]
PHST- 2018/01/14 00:00 [revised]
PHST- 2018/02/12 00:00 [accepted]
PHST- 2018/03/25 06:00 [pubmed]
PHST- 2019/02/09 06:00 [medline]
PHST- 2018/03/25 06:00 [entrez]
AID - 10.1002/lary.27164 [doi]
PST - ppublish
SO  - Laryngoscope. 2018 Sep;128(9):E316-E322. doi: 10.1002/lary.27164. Epub 2018 Mar 
      23.