PMID- 29572935
OWN - NLM
STAT- MEDLINE
DCOM- 20181004
LR  - 20210109
IS  - 1939-1676 (Electronic)
IS  - 0891-6640 (Print)
IS  - 0891-6640 (Linking)
VI  - 32
IP  - 3
DP  - 2018 May
TI  - Effect of interleukin-1beta on occludin mRNA expression in the duodenal and colonic 
      mucosa of dogs with inflammatory bowel disease.
PG  - 1019-1025
LID - 10.1111/jvim.15117 [doi]
AB  - BACKGROUND: Mucosal imbalance of interleukin (IL)-1beta and IL-1 receptor antagonist 
      (Ra) has been reported in the duodenal mucosa of dogs with inflammatory bowel 
      disease (IBD). However, the imbalance in the colonic mucosa and its role in 
      duodenitis and colitis in IBD of dogs remain unclear. OBJECTIVES: To measure the 
      expression of IL-1beta and IL-1Ra proteins in the colonic mucosa of dogs with IBD, 
      and to determine the effect of IL-1beta on expression of occludin (ocln) mRNA, a 
      tight junction component, in the duodenal and colonic mucosa of dogs with IBD. 
      ANIMALS: Twelve dogs with IBD and 6 healthy dogs. METHODS: IL-1beta and IL-1 Ra 
      proteins in the colonic mucosa were quantified by ELISA in 7 of the 12 dogs with 
      IBD. Expression of ocln mRNA in the duodenal and colonic mucosa was examined in 
      the 12 dogs by real-time PCR. RESULTS: The ratio of IL-1beta to IL-1Ra in the 
      colonic mucosa was significantly higher in dogs with IBD than in healthy dogs. 
      The ex vivo experiment determined that IL-1beta suppressed expression of ocln mRNA 
      in the colonic mucosa, but not in the duodenal mucosa, of healthy dogs. 
      Expression of ocln mRNA in the colonic mucosa, but not in the duodenal mucosa, 
      was significantly lower in dogs with IBD than in healthy dogs. CONCLUSIONS AND 
      CLINICAL IMPORTANCE: A relative increase in IL-1beta may attenuate ocln expression, 
      leading to intestinal barrier dysfunction and promotion of intestinal 
      inflammation in the colonic mucosa, but not in the duodenal mucosa, of dogs with 
      IBD.
CI  - Copyright (c) 2018 The Authors. Journal of Veterinary Internal Medicine published 
      by Wiley Periodicals, Inc. on behalf of the American College of Veterinary 
      Internal Medicine.
FAU - Ogawa, Misato
AU  - Ogawa M
AD  - Cooperative Department of Veterinary Medicine, Tokyo University of Agriculture 
      and Technology, Fuchu, Tokyo, Japan.
FAU - Osada, Hironari
AU  - Osada H
AD  - Cooperative Department of Veterinary Medicine, Tokyo University of Agriculture 
      and Technology, Fuchu, Tokyo, Japan.
FAU - Hasegawa, Ayana
AU  - Hasegawa A
AD  - Cooperative Department of Veterinary Medicine, Tokyo University of Agriculture 
      and Technology, Fuchu, Tokyo, Japan.
FAU - Ohno, Hikaru
AU  - Ohno H
AD  - Cooperative Department of Veterinary Medicine, Tokyo University of Agriculture 
      and Technology, Fuchu, Tokyo, Japan.
FAU - Yanuma, Nanako
AU  - Yanuma N
AD  - Cooperative Department of Veterinary Medicine, Tokyo University of Agriculture 
      and Technology, Fuchu, Tokyo, Japan.
FAU - Sasaki, Kazuaki
AU  - Sasaki K
AD  - Cooperative Department of Veterinary Medicine, Tokyo University of Agriculture 
      and Technology, Fuchu, Tokyo, Japan.
FAU - Shimoda, Minoru
AU  - Shimoda M
AD  - Cooperative Department of Veterinary Medicine, Tokyo University of Agriculture 
      and Technology, Fuchu, Tokyo, Japan.
FAU - Shirai, Junsuke
AU  - Shirai J
AD  - Cooperative Department of Veterinary Medicine, Tokyo University of Agriculture 
      and Technology, Fuchu, Tokyo, Japan.
FAU - Kondo, Hirotaka
AU  - Kondo H
AD  - Laboratory of Veterinary Pathology, Department of Veterinary Medicine, College of 
      Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan.
FAU - Ohmori, Keitaro
AU  - Ohmori K
AUID- ORCID: 0000-0002-0582-4880
AD  - Cooperative Department of Veterinary Medicine, Tokyo University of Agriculture 
      and Technology, Fuchu, Tokyo, Japan.
LA  - eng
PT  - Journal Article
DEP - 20180323
PL  - United States
TA  - J Vet Intern Med
JT  - Journal of veterinary internal medicine
JID - 8708660
RN  - 0 (Interleukin-1beta)
RN  - 0 (Occludin)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Interleukin-1)
SB  - IM
MH  - Animals
MH  - Case-Control Studies
MH  - Colon/*metabolism
MH  - Dog Diseases/*metabolism
MH  - Dogs
MH  - Duodenum/*metabolism
MH  - Female
MH  - Gene Expression
MH  - Inflammatory Bowel Diseases/metabolism/*veterinary
MH  - Interleukin-1beta/*metabolism
MH  - Intestinal Mucosa/*metabolism
MH  - Male
MH  - Occludin/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Receptors, Interleukin-1/metabolism
MH  - Tight Junctions/metabolism
PMC - PMC5980446
OTO - NOTNLM
OT  - chronic enteropathy
OT  - inflammatory cytokine
OT  - intestinal barrier dysfunction
OT  - tight junction
EDAT- 2018/03/25 06:00
MHDA- 2018/10/05 06:00
PMCR- 2018/05/01
CRDT- 2018/03/25 06:00
PHST- 2017/10/18 00:00 [received]
PHST- 2018/02/06 00:00 [revised]
PHST- 2018/02/14 00:00 [accepted]
PHST- 2018/03/25 06:00 [pubmed]
PHST- 2018/10/05 06:00 [medline]
PHST- 2018/03/25 06:00 [entrez]
PHST- 2018/05/01 00:00 [pmc-release]
AID - JVIM15117 [pii]
AID - 10.1111/jvim.15117 [doi]
PST - ppublish
SO  - J Vet Intern Med. 2018 May;32(3):1019-1025. doi: 10.1111/jvim.15117. Epub 2018 
      Mar 23.