PMID- 29573248
OWN - NLM
STAT- MEDLINE
DCOM- 20180829
LR  - 20220408
IS  - 1598-6357 (Electronic)
IS  - 1011-8934 (Print)
IS  - 1011-8934 (Linking)
VI  - 33
IP  - 13
DP  - 2018 Mar 26
TI  - Comparison between Newly Developed and Commercial Inhalant Skin Prick Test 
      Reagents Using In Vivo and In Vitro Methods.
PG  - e101
LID - 10.3346/jkms.2018.33.e101 [doi]
LID - e101
AB  - BACKGROUND: We developed skin prick test (SPT) reagents for common inhalant 
      allergens that reflected the real exposure in Korea. The study aim was to 
      evaluate diagnostic usefulness and allergen potency of our inhalant SPT reagents 
      in comparison with commercial products. METHODS: We produced eight common 
      inhalant allergen SPT reagents using total extract (Prolagen): Dermatophagoides 
      farinae, Dermatophagoides pteronyssinus, oak, ragweed, mugwort, Humulus japonicus 
      pollens, as well as cat and dog allergens. We compared the newly developed 
      reagents with three commercially available SPT reagents (Allergopharma, 
      Hollister-Stier, Lofarma). We measured total protein concentrations, sodium 
      dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), major allergen 
      concentration, and biological allergen potencies measured by immunoglobulin E 
      (IgE) immunoblotting and ImmunoCAP inhibition test. RESULTS: Diagnostic values of 
      these SPT reagents were expressed as positivity rate and concordance rate of the 
      results from ImmunoCAP allergen-specific IgE test in 94 allergic patients. In 
      vitro analysis showed marked differences in protein concentrations, SDS-PAGE 
      features, major allergen concentrations, and biological allergen potencies of 
      four different SPT reagents. In vivo analysis showed that positive rates and 
      concordance rates of Prolagen(R) SPT reagents were similar compared to the three 
      commercial SPT reagents. CONCLUSION: The newly developed Prolagen(R) inhalant SPT 
      reagents are not inferior to the commercially available SPT reagents in allergy 
      diagnosis.
CI  - (c) 2018 The Korean Academy of Medical Sciences.
FAU - Lee, Sang Chul
AU  - Lee SC
AUID- ORCID: 0000-0001-5318-6804
AD  - Division of Allergy and Immunology, Department of Internal Medicine, Yonsei 
      University College of Medicine, Seoul, Korea.
AD  - Institute of Allergy, Yonsei University College of Medicine, Seoul, Korea.
FAU - Sim, Da Woon
AU  - Sim DW
AUID- ORCID: 0000-0002-9723-0720
AD  - Division of Allergy, Department of Internal Medicine, Chonnam National University 
      Medical School, Gwangju, Korea.
FAU - Lee, Jongsun
AU  - Lee J
AUID- ORCID: 0000-0003-1526-2241
AD  - Institute of Allergy, Yonsei University College of Medicine, Seoul, Korea.
FAU - Jeong, Kyoung Yong
AU  - Jeong KY
AUID- ORCID: 0000-0001-9887-1426
AD  - Institute of Allergy, Yonsei University College of Medicine, Seoul, Korea.
FAU - Park, Kyung Hee
AU  - Park KH
AUID- ORCID: 0000-0003-3605-5364
AD  - Division of Allergy and Immunology, Department of Internal Medicine, Yonsei 
      University College of Medicine, Seoul, Korea.
AD  - Institute of Allergy, Yonsei University College of Medicine, Seoul, Korea.
FAU - Lee, Jae Hyun
AU  - Lee JH
AUID- ORCID: 0000-0002-0760-0071
AD  - Division of Allergy and Immunology, Department of Internal Medicine, Yonsei 
      University College of Medicine, Seoul, Korea.
AD  - Institute of Allergy, Yonsei University College of Medicine, Seoul, Korea.
FAU - Kim, Jung Dong
AU  - Kim JD
AUID- ORCID: 0000-0002-6348-9267
AD  - Prolagen, Seoul, Korea.
FAU - Park, Jung Won
AU  - Park JW
AUID- ORCID: 0000-0003-0249-8749
AD  - Division of Allergy and Immunology, Department of Internal Medicine, Yonsei 
      University College of Medicine, Seoul, Korea.
AD  - Institute of Allergy, Yonsei University College of Medicine, Seoul, Korea.
AD  - Prolagen, Seoul, Korea. PARKJW@yuhs.ac.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20180326
PL  - Korea (South)
TA  - J Korean Med Sci
JT  - Journal of Korean medical science
JID - 8703518
RN  - 0 (Allergens)
RN  - 0 (Reagent Kits, Diagnostic)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adult
MH  - Allergens/analysis/*immunology
MH  - Double-Blind Method
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Humans
MH  - Hypersensitivity/*diagnosis
MH  - Immunoglobulin E/analysis
MH  - Male
MH  - Middle Aged
MH  - Prospective Studies
MH  - Reagent Kits, Diagnostic
MH  - Skin Tests/*methods
PMC - PMC5865053
OTO - NOTNLM
OT  - Allergens
OT  - Allergy and Immunology
OT  - Diagnosis
OT  - Skin Prick Test
COIS- Dr. Park JW reports serving as an unpaid chief technology officer for Prolagen; 
      Dr. Kim JD, serving as chief executive officer for Prolagen. Dr. Park JW, Dr. Kim 
      JD, Dr. Park KH, having an equity interest in Prolagen. These authors' interests 
      did not influence academic fairness in conducting this study, analyzing results, 
      and writing a paper. Other authors have no potential conflicts of interest to 
      disclose.
EDAT- 2018/03/25 06:00
MHDA- 2018/08/30 06:00
PMCR- 2018/03/26
CRDT- 2018/03/25 06:00
PHST- 2017/09/26 00:00 [received]
PHST- 2018/01/19 00:00 [accepted]
PHST- 2018/03/25 06:00 [entrez]
PHST- 2018/03/25 06:00 [pubmed]
PHST- 2018/08/30 06:00 [medline]
PHST- 2018/03/26 00:00 [pmc-release]
AID - 33.e101 [pii]
AID - 10.3346/jkms.2018.33.e101 [doi]
PST - epublish
SO  - J Korean Med Sci. 2018 Mar 26;33(13):e101. doi: 10.3346/jkms.2018.33.e101.