PMID- 29573663
OWN - NLM
STAT- MEDLINE
DCOM- 20180928
LR  - 20211204
IS  - 1879-3231 (Electronic)
IS  - 0093-691X (Linking)
VI  - 113
DP  - 2018 Jun
TI  - Capsaicin protects against testicular torsion injury through mTOR-dependent 
      mechanism.
PG  - 247-252
LID - S0093-691X(18)30093-1 [pii]
LID - 10.1016/j.theriogenology.2018.03.012 [doi]
AB  - SCOPE: Testicular torsion and subsequent release of reactive oxygen species (ROS) 
      can cause infertility in adults. Oxidative stress following testicular torsion 
      plays an important role in the yonset and development of apoptotic cell death 
      through dysregulation of the cellular signaling pathways. Anti-inflammatory and 
      antioxidant properties of capsaicin, a bioactive composition present in red 
      peppers, has already been exploited for treatment of the cancer and pain relief. 
      In present work, we evaluated the role of the mammalian target of rapamycin 
      (mTOR) in antioxidant effect of capsaicin against reperfusion injury following 
      testicular torsion. METHODS: Male Wistar rats weighing 200-220 g were randomly 
      assigned into four major groups: (i) a sham operated group, (ii) a testicular 
      torsion (TT) group, (iii) three groups treated with different doses of capsaicin 
      (TT + 100, 500 and 1000 mug/ml Cap), and (iv) three groups of healthy rats treated 
      with different doses of capsaicin (100, 500 and 1000 mug/ml). Western blotting 
      assay was used to examine the anti-apoptotic effects of capsaicin in testicular 
      cells following torsion. H&E and TUNEL methods were used to evaluate testicular 
      morphology and apoptosis activity. RESULTS: Compared to control group, 
      phosphorylation of mTOR was significantly increased in the TT groups. Capsaicin 
      administration remarkably decreased the phosphorylation of mTOR at the highest 
      dose (P < 0.05). Capsaicin decreased apoptosis and preserved tubular morphology 
      in testes. CONCLUSION: Our results showed that antioxidant properties of 
      capsaicin minimizes cell death and reperfusion injury following testicular 
      torsion.
CI  - Copyright (c) 2018. Published by Elsevier Inc.
FAU - Javdan, Nasim
AU  - Javdan N
AD  - ShahidBeheshti University of Medical Sciences, Phytochemistry Research Center, 
      Tehran, Iran.
FAU - Ayatollahi, Seyed Abdulmajid
AU  - Ayatollahi SA
AD  - ShahidBeheshti University of Medical Sciences, Phytochemistry Research Center, 
      Tehran, Iran; Department of Chemistry, Richardson College for the Environmental 
      Science Complex, The University of Winnipeg, 599 Portage Avenue, Winnipeg, MB, 
      R3B 2G3, Canada; School of Pharmacy, ShahidBeheshti University of Medical 
      Sciences, Tehran, Iran. Electronic address: majid_ayatollahi@yahoo.com.
FAU - Choudhary, Muhammad Iqbal
AU  - Choudhary MI
AD  - International Center for Chemical and Biological Sciences, H.E.J. Research 
      Institute of Chemistry, University of Karachi, Karachi, Pakistan.
FAU - Al-Hasani, Safaa
AU  - Al-Hasani S
AD  - Reproductive Medicine Unit, University of Schleswig-Holstein, Luebeck, Germany.
FAU - Kobarfard, Farzad
AU  - Kobarfard F
AD  - Department of Medicinal Chemistry, Shaheed Beheshti School of Pharmacy, Tehran, 
      Iran.
FAU - Athar, Ata
AU  - Athar A
AD  - Department of Chemistry, Richardson College for the Environmental Science 
      Complex, The University of Winnipeg, 599 Portage Avenue, Winnipeg, MB, R3B 2G3, 
      Canada.
FAU - Pazoki-Toroudi, Hamidreza
AU  - Pazoki-Toroudi H
AD  - Physiology Research Center and Department of Physiology, Faculty of Medicine, 
      Iran University of Medical Sciences, Tehran, Iran. Electronic address: 
      pazoki49@gmail.com.
LA  - eng
PT  - Journal Article
DEP - 20180315
PL  - United States
TA  - Theriogenology
JT  - Theriogenology
JID - 0421510
RN  - 0 (Antipruritics)
RN  - EC 2.7.1.1 (mTOR protein, rat)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - S07O44R1ZM (Capsaicin)
SB  - IM
MH  - Animals
MH  - Antipruritics/pharmacology
MH  - Capsaicin/*pharmacology
MH  - DNA Fragmentation
MH  - Gene Expression Regulation/*drug effects
MH  - Male
MH  - Rats
MH  - Rats, Wistar
MH  - Spermatic Cord Torsion/drug therapy/pathology/*veterinary
MH  - TOR Serine-Threonine Kinases/genetics/*metabolism
MH  - Testis/metabolism/pathology
OTO - NOTNLM
OT  - Apoptosis
OT  - Capsaicin
OT  - Cell survival
OT  - Testicular torsion
OT  - mTOR pathway
EDAT- 2018/03/25 06:00
MHDA- 2018/10/03 06:00
CRDT- 2018/03/25 06:00
PHST- 2017/12/19 00:00 [received]
PHST- 2018/03/10 00:00 [revised]
PHST- 2018/03/11 00:00 [accepted]
PHST- 2018/03/25 06:00 [pubmed]
PHST- 2018/10/03 06:00 [medline]
PHST- 2018/03/25 06:00 [entrez]
AID - S0093-691X(18)30093-1 [pii]
AID - 10.1016/j.theriogenology.2018.03.012 [doi]
PST - ppublish
SO  - Theriogenology. 2018 Jun;113:247-252. doi: 10.1016/j.theriogenology.2018.03.012. 
      Epub 2018 Mar 15.