PMID- 29573867
OWN - NLM
STAT- MEDLINE
DCOM- 20181015
LR  - 20181015
IS  - 1618-0631 (Electronic)
IS  - 0344-0338 (Linking)
VI  - 214
IP  - 4
DP  - 2018 Apr
TI  - Brain-derived neurotrophic factor induces angiogenin secretion and nuclear 
      translocation in human umbilical vein endothelial cells.
PG  - 521-526
LID - S0344-0338(17)31005-1 [pii]
LID - 10.1016/j.prp.2018.02.013 [doi]
AB  - Brain-derived neurotrophic factor (BDNF) is a well-known humoral protein that 
      induces growth of neurons. Recent studies have suggested that BDNF could act as 
      an angiogenesis inducer similar to vascular endothelial growth factor (VEGF). 
      Angiogenin is a strong mediator of angiogenesis. It has particular 
      characteristics both as a secreted protein and a transcription factor. After 
      being incorporated into the cytoplasm, angiogenin is immediately transferred to 
      the nucleus and then mediates the angiogenic effects of angiogenesis inducers, 
      including VEGF. The aim of this study is to determine the association between 
      BDNF and angiogenin. At first, we determined the secretion of angiogenin from 
      human umbilical vein endothelial cells (HUVEC) induced by BDNF with enzyme-linked 
      immunosorbent assay. Next, we determined BDNF-induced nuclear translocation of 
      angiogenin by immunofluorescent staining. In addition, we examined the mRNA 
      expression of angiogenin in HUVEC before and after BDNF stimulation by 
      quantitative reverse transcriptase-polymerase chain reaction. As a result, we 
      noted that BDNF induced angiogenin secretion and nuclear translocation without an 
      increase in the mRNA expression in HUVEC. Furthermore, we demonstrated that 
      BDNF-induced HUVEC proliferation was significantly suppressed when neomycin, a 
      specific inhibitor of nuclear translocation of angiogenin, was administered. 
      These findings indicate that nuclear translocation of angiogenin is critically 
      involved in BDNF-induced proliferation of HUVEC. In conclusion, angiogenin 
      contributes to angiogenesis induced by BDNF.
CI  - Copyright (c) 2018 Elsevier GmbH. All rights reserved.
FAU - Mori, Ayako
AU  - Mori A
AD  - Graduate School of Health Sciences, Hokkaido University, Sapporo, Japan.
FAU - Nishioka, Yusuke
AU  - Nishioka Y
AD  - Graduate School of Health Sciences, Hokkaido University, Sapporo, Japan.
FAU - Yamada, Mai
AU  - Yamada M
AD  - Graduate School of Health Sciences, Hokkaido University, Sapporo, Japan.
FAU - Nishibata, Yuka
AU  - Nishibata Y
AD  - Faculty of Health Sciences, Hokkaido University, Sapporo, Japan.
FAU - Masuda, Sakiko
AU  - Masuda S
AD  - Faculty of Health Sciences, Hokkaido University, Sapporo, Japan.
FAU - Tomaru, Utano
AU  - Tomaru U
AD  - Department of Pathology, Faculty of Medicine and Graduate School of Medicine, 
      Hokkaido University, Sapporo, Japan.
FAU - Honma, Naoyuki
AU  - Honma N
AD  - Faculty of Health Sciences, Hokkaido University, Sapporo, Japan.
FAU - Moriyama, Takanori
AU  - Moriyama T
AD  - Faculty of Health Sciences, Hokkaido University, Sapporo, Japan.
FAU - Ishizu, Akihiro
AU  - Ishizu A
AD  - Faculty of Health Sciences, Hokkaido University, Sapporo, Japan. Electronic 
      address: aishizu@med.hokudai.ac.jp.
LA  - eng
PT  - Journal Article
DEP - 20180221
PL  - Germany
TA  - Pathol Res Pract
JT  - Pathology, research and practice
JID - 7806109
RN  - 0 (Angiogenesis Inducing Agents)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (VEGFA protein, human)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 7171WSG8A2 (BDNF protein, human)
RN  - EC 3.1.27.- (angiogenin)
RN  - EC 3.1.27.5 (Ribonuclease, Pancreatic)
SB  - IM
MH  - Angiogenesis Inducing Agents/*metabolism
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Cell Nucleus/metabolism
MH  - Human Umbilical Vein Endothelial Cells
MH  - Humans
MH  - Neovascularization, Pathologic
MH  - Neovascularization, Physiologic
MH  - Ribonuclease, Pancreatic/*metabolism
MH  - Vascular Endothelial Growth Factor A/metabolism
OTO - NOTNLM
OT  - Angiogenesis
OT  - Angiogenin
OT  - BDNF
OT  - VEGF
EDAT- 2018/03/27 06:00
MHDA- 2018/10/16 06:00
CRDT- 2018/03/26 06:00
PHST- 2017/10/06 00:00 [received]
PHST- 2018/01/25 00:00 [revised]
PHST- 2018/02/14 00:00 [accepted]
PHST- 2018/03/27 06:00 [pubmed]
PHST- 2018/10/16 06:00 [medline]
PHST- 2018/03/26 06:00 [entrez]
AID - S0344-0338(17)31005-1 [pii]
AID - 10.1016/j.prp.2018.02.013 [doi]
PST - ppublish
SO  - Pathol Res Pract. 2018 Apr;214(4):521-526. doi: 10.1016/j.prp.2018.02.013. Epub 
      2018 Feb 21.