PMID- 29576706
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220316
IS  - 0973-1296 (Print)
IS  - 0976-4062 (Electronic)
IS  - 0973-1296 (Linking)
VI  - 14
IP  - 53
DP  - 2018 Jan-Mar
TI  - Rhynchophylline Downregulates Phosphorylated cAMP Response Element Binding 
      Protein, Nuclear Receptor-related-1, and Brain-derived Neurotrophic Factor 
      Expression in the Hippocampus of Ketamine-induced Conditioned Place Preference 
      Rats.
PG  - 81-86
LID - 10.4103/pm.pm_90_17 [doi]
AB  - BACKGROUND: Addiction to ketamine is becoming a serious public health issues, for 
      which there exists no effective treatment. Rhynchophylline (Rhy) is an alkaloid 
      extracted from certain Uncaria species that is well known for both its potent 
      anti-addictive and neuroprotective properties. Increasing evidence supports the 
      contributions of cAMP response element binding protein (CREB), nuclear 
      receptor-related-1 (Nurr1), and brain-derived neurotrophic factor (BDNF) in 
      modulating neural and behavioral plasticity which was induced by addictive drugs. 
      OBJECTIVE: To investigate the effects of Rhy on the behavior and the levels of 
      phosphorylated CREB (p-CREB), Nurr1, and BDNF in the hippocampus of 
      ketamine-induced conditioned place preference (CPP) rats. MATERIALS AND METHODS: 
      CPP paradigm was used to establish the model of ketamine-dependent rats and to 
      evaluate the effect of Rhy on ketamine dependence. The expressions of p-CREB, 
      Nurr1, and BDNF were tested by Western blotting and immunohistochemistry. 
      RESULTS: We observed that Rhy can reverse the behavior preference induced by 
      ketamine CPP training. At the same time, expression of p-CREB, Nurr1, and BDNF, 
      which was significantly increased by ketamine, was restored in the Rhy -treated 
      group. CONCLUSION: This study indicates that Rhy can reverse the reward effect 
      induced by ketamine in rats and the mechanism can probably be related to regulate 
      the hippocampal protein expression of p-CREB, Nurr1, and BDNF. SUMMARY: P-CREB, 
      Nurr1 and BDNF play an important role in the formation of ketamine-induced place 
      preference in ratsRhynchophylline reversed the expression of p-CREB, Nurr1 and 
      BDNF which was activated by ketamine in the hippocampusRhynchophylline 
      demonstrates the potential effect of mediates ketamine induced rewarding effect. 
      Abbreviations used: Rhy: Rhynchophylline; CREB: cAMP response element binding 
      protein; Nurr1: Nuclear receptor-related-1; BDNF: Brain-derived neurotrophic 
      factor; CPP: Conditioned place preference; NMDA: N-methyl-D-aspartic acid; METH: 
      Methamphetamine; CNS: Central nervous system; PFA: Paraformaldehyde; GAPDH: 
      Glyceraldehyde-3-phosphate dehydrogenase; LTP: long-term potentiation.
FAU - Guo, Youli
AU  - Guo Y
AD  - School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, 
      China.
AD  - Department of Pharmacy, Guangdong Provincial Corps Hospital of Chinese People's 
      Armed Police Forces, Guangzhou, China.
FAU - Luo, Chaohua
AU  - Luo C
AD  - School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, 
      China.
FAU - Tu, Genghong
AU  - Tu G
AD  - Department of Pathophysiology, Guangdong Province Key Laboratory of Functional 
      Proteomics, Southern Medical University, Guangzhou, China.
FAU - Li, Chan
AU  - Li C
AD  - School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, 
      China.
FAU - Liu, Yi
AU  - Liu Y
AD  - School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, 
      China.
FAU - Liu, Wei
AU  - Liu W
AD  - School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, 
      China.
FAU - Lam Yung, Ken Kin
AU  - Lam Yung KK
AD  - Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong, 
      China.
FAU - Mo, Zhixian
AU  - Mo Z
AD  - School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20180220
PL  - United States
TA  - Pharmacogn Mag
JT  - Pharmacognosy magazine
JID - 101300403
PMC - PMC5858247
OTO - NOTNLM
OT  - Brain-derived neurotrophic factor
OT  - conditioned place preference
OT  - ketamine
OT  - nuclear receptor-related-1
OT  - phosphorylated cAMP response element binding protein
OT  - rhynchophylline
COIS- There are no conflicts of interest.
EDAT- 2018/03/27 06:00
MHDA- 2018/03/27 06:01
PMCR- 2018/01/01
CRDT- 2018/03/27 06:00
PHST- 2017/03/05 00:00 [received]
PHST- 2017/03/31 00:00 [revised]
PHST- 2018/03/27 06:00 [entrez]
PHST- 2018/03/27 06:00 [pubmed]
PHST- 2018/03/27 06:01 [medline]
PHST- 2018/01/01 00:00 [pmc-release]
AID - PM-14-81 [pii]
AID - 10.4103/pm.pm_90_17 [doi]
PST - ppublish
SO  - Pharmacogn Mag. 2018 Jan-Mar;14(53):81-86. doi: 10.4103/pm.pm_90_17. Epub 2018 
      Feb 20.