PMID- 29577646
OWN - NLM
STAT- MEDLINE
DCOM- 20190906
LR  - 20240314
IS  - 2045-7634 (Electronic)
IS  - 2045-7634 (Linking)
VI  - 7
IP  - 5
DP  - 2018 May
TI  - Performance of the UroVysion((R)) FISH assay for the diagnosis of malignant 
      effusions using two cutoff strategies.
PG  - 1967-1977
LID - 10.1002/cam4.1442 [doi]
AB  - The cytological examination of cavity fluids has limited sensitivity in the 
      diagnosis of malignancy. Aneuploidy, which is commonly observed in neoplastic 
      cells, could potentially be used as an ancillary diagnostic tool. To evaluate the 
      detection of aneuploid cells in cavitary effusion samples using the fluorescence 
      in situ hybridization (FISH) assay UroVysion((R)) with some adaptations and two 
      different cutoff strategies. Seventy samples of pleural or peritoneal fluid with 
      positive (n = 40), negative (n = 15), or suspicious (n = 15) oncotic cytology 
      were subjected to FISH assay with the multitarget UroVysion((R)) kit, which is 
      composed of probes that hybridize to the centromeric region of chromosomes 3, 7, 
      and 17 and to the locus 9p21. FISH performance was evaluated using two different 
      cutoffs: (1) the manufacturer's cutoff (M-FISH) and 2) a proposed cutoff 
      (P-FISH). Using M-FISH, the diagnostic sensitivity was 57.1%, specificity 87.5%, 
      and accuracy 60.0%; with P-FISH, the sensitivity was 87.3%, specificity 71.4%, 
      and accuracy 85.7%. When combined with cytology, the sensitivity, specificity, 
      and accuracy were 88.0%, 83.3%, and 87.8%, respectively. Malignant cells 
      presented a predominance of chromosomal gains. The UroVysion((R)) test using the 
      P-FISH cutoff was effective in demonstrating aneuploid cells in all malignant 
      effusions, confirming the diagnosis of malignancy even in cases with suspicious 
      cytology.
CI  - (c) 2018 The Authors. Cancer Medicine published by John Wiley & Sons Ltd.
FAU - Rosolen, Debora C B
AU  - Rosolen DCB
AUID- ORCID: 0000-0003-3785-2816
AD  - Division of Clinical Pathology, Department of Pathology, Faculdade de Medicina, 
      Hospital das Clinicas HCFMUSP, Universidade de Sao Paulo, Sao Paulo, SP, Brazil.
FAU - Faria, Daniel K
AU  - Faria DK
AD  - Division of Clinical Pathology, Department of Pathology, Faculdade de Medicina, 
      Hospital das Clinicas HCFMUSP, Universidade de Sao Paulo, Sao Paulo, SP, Brazil.
FAU - Faria, Caroline S
AU  - Faria CS
AD  - Faculdade de Medicina, Medical Investigation Laboratory (LIM03), Hospital das 
      Clinicas HCFMUSP, Universidade de Sao Paulo, Sao Paulo, SP, Brazil.
FAU - Antonangelo, Leila
AU  - Antonangelo L
AD  - Division of Clinical Pathology, Department of Pathology, Faculdade de Medicina, 
      Hospital das Clinicas HCFMUSP, Universidade de Sao Paulo, Sao Paulo, SP, Brazil.
AD  - Faculdade de Medicina, Medical Investigation Laboratory (LIM03), Hospital das 
      Clinicas HCFMUSP, Universidade de Sao Paulo, Sao Paulo, SP, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20180325
PL  - United States
TA  - Cancer Med
JT  - Cancer medicine
JID - 101595310
SB  - IM
MH  - Aged
MH  - Aneuploidy
MH  - Chromosome Aberrations
MH  - Cytogenetic Analysis
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Middle Aged
MH  - Pleural Effusion, Malignant/*diagnosis/genetics
MH  - Sensitivity and Specificity
PMC - PMC5943432
OTO - NOTNLM
OT  - Aneuploidy
OT  - ascites
OT  - cytology
OT  - fluorescent in situ hybridization
OT  - malignancy
OT  - pleural effusion
EDAT- 2018/03/27 06:00
MHDA- 2019/09/07 06:00
PMCR- 2018/03/25
CRDT- 2018/03/27 06:00
PHST- 2018/01/08 00:00 [received]
PHST- 2018/02/09 00:00 [revised]
PHST- 2018/02/17 00:00 [accepted]
PHST- 2018/03/27 06:00 [pubmed]
PHST- 2019/09/07 06:00 [medline]
PHST- 2018/03/27 06:00 [entrez]
PHST- 2018/03/25 00:00 [pmc-release]
AID - CAM41442 [pii]
AID - 10.1002/cam4.1442 [doi]
PST - ppublish
SO  - Cancer Med. 2018 May;7(5):1967-1977. doi: 10.1002/cam4.1442. Epub 2018 Mar 25.