PMID- 29577884
OWN - NLM
STAT- MEDLINE
DCOM- 20190401
LR  - 20190401
IS  - 1872-6240 (Electronic)
IS  - 0006-8993 (Linking)
VI  - 1688
DP  - 2018 Jun 1
TI  - Regional-specific effects of cerebral ischemia/reperfusion and 
      dehydroepiandrosterone on synaptic NMDAR/PSD-95 complex in male Wistar rats.
PG  - 73-80
LID - S0006-8993(18)30158-6 [pii]
LID - 10.1016/j.brainres.2018.03.023 [doi]
AB  - Excessive glutamate efflux and N-methyl-D-aspartate receptor (NMDAR) 
      over-activation represent well-known hallmarks of cerebral ischemia/reperfusion 
      (I/R) injury, still, expression of proteins involved in this aspect of I/R 
      pathophysiology show inconsistent data. Neurosteroid dehydroepiandrosterone 
      (DHEA) has been proposed as potent NMDAR modulator, but its influence on 
      I/R-induced changes up to date remains questionable. Therefore, I/R-governed 
      alteration of vesicular glutamate transporter 1 (vGluT1), synaptic NMDAR subunit 
      composition, postsynaptic density protein 95 (PSD-95) and neuronal morphology 
      alone or following DHEA treatment were examined. For that purpose, adult male 
      Wistar rats were treated with a single dose of vehicle or DHEA (20 mg/kg i.p.) 
      4 h following sham operation or 15 min bilateral common carotid artery occlusion. 
      Western blot was used for analyses of synaptic protein expressions in hippocampus 
      and prefrontal cortex, while neuronal morphology was assessed using Nissl 
      staining. Regional-specific postischemic changes were detected on protein level 
      i.e. signs of neuronal damage in CA1 area was accompanied with hippocampal 
      vGluT1, NR1, NR2B enhancement and PSD-95 decrement, while histological changes 
      observed in layer III were associated with decreased NR1 subunit in prefrontal 
      cortex. Under physiological conditions DHEA had no effect on protein and 
      histological appearance, while in ischemic milieu it restored hippocampal PSD-95 
      and NR1 in prefrontal cortex to the control level. Along with intact neurons, 
      ones characterized by morphology observed in I/R group were also present. Future 
      studies involving NMDAR-related intracellular signaling and immunohistochemical 
      analysis will reveal precise effects of I/R and DHEA treatment in selected brain 
      regions.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Zaric, Marina
AU  - Zaric M
AD  - Department of Molecular Biology and Endocrinology, Vinca Institute of Nuclear 
      Sciences, University of Belgrade, Belgrade, Serbia.
FAU - Drakulic, Dunja
AU  - Drakulic D
AD  - Department of Molecular Biology and Endocrinology, Vinca Institute of Nuclear 
      Sciences, University of Belgrade, Belgrade, Serbia.
FAU - Stojanovic, Ivana Gusevac
AU  - Stojanovic IG
AD  - Department of Molecular Biology and Endocrinology, Vinca Institute of Nuclear 
      Sciences, University of Belgrade, Belgrade, Serbia.
FAU - Mitrovic, Natasa
AU  - Mitrovic N
AD  - Department of Molecular Biology and Endocrinology, Vinca Institute of Nuclear 
      Sciences, University of Belgrade, Belgrade, Serbia.
FAU - Grkovic, Ivana
AU  - Grkovic I
AD  - Department of Molecular Biology and Endocrinology, Vinca Institute of Nuclear 
      Sciences, University of Belgrade, Belgrade, Serbia.
FAU - Martinovic, Jelena
AU  - Martinovic J
AD  - Department of Molecular Biology and Endocrinology, Vinca Institute of Nuclear 
      Sciences, University of Belgrade, Belgrade, Serbia. Electronic address: 
      jelenazlatkovic@vinca.rs.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180322
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Disks Large Homolog 4 Protein)
RN  - 0 (Dlg4 protein, rat)
RN  - 0 (NR2B NMDA receptor)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Protein Subunits)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 0 (Slc17a7 protein, rat)
RN  - 0 (Vesicular Glutamate Transport Protein 1)
RN  - 459AG36T1B (Dehydroepiandrosterone)
SB  - IM
MH  - Animals
MH  - Brain Ischemia/*metabolism/prevention & control
MH  - Dehydroepiandrosterone/*administration & dosage/metabolism
MH  - Disks Large Homolog 4 Protein/*metabolism
MH  - Hippocampus/drug effects/*metabolism
MH  - Male
MH  - Neuroprotective Agents/*administration & dosage
MH  - Prefrontal Cortex/drug effects/*metabolism
MH  - Protein Subunits/metabolism
MH  - Rats, Wistar
MH  - Receptors, N-Methyl-D-Aspartate/*metabolism
MH  - Reperfusion Injury/metabolism/prevention & control
MH  - Synapses/drug effects/*metabolism
MH  - Synaptosomes/metabolism
MH  - Vesicular Glutamate Transport Protein 1/metabolism
OTO - NOTNLM
OT  - Cerebral ischemia/reperfusion
OT  - Dehydroepiandrosterone
OT  - Glutamatergic neurotransmission
OT  - Hippocampus
OT  - Prefrontal cortex
OT  - Synaptosomes
EDAT- 2018/03/27 06:00
MHDA- 2019/04/02 06:00
CRDT- 2018/03/27 06:00
PHST- 2018/01/07 00:00 [received]
PHST- 2018/03/16 00:00 [revised]
PHST- 2018/03/17 00:00 [accepted]
PHST- 2018/03/27 06:00 [pubmed]
PHST- 2019/04/02 06:00 [medline]
PHST- 2018/03/27 06:00 [entrez]
AID - S0006-8993(18)30158-6 [pii]
AID - 10.1016/j.brainres.2018.03.023 [doi]
PST - ppublish
SO  - Brain Res. 2018 Jun 1;1688:73-80. doi: 10.1016/j.brainres.2018.03.023. Epub 2018 
      Mar 22.