PMID- 29579552
OWN - NLM
STAT- MEDLINE
DCOM- 20190221
LR  - 20190221
IS  - 1525-5069 (Electronic)
IS  - 1525-5050 (Linking)
VI  - 82
DP  - 2018 May
TI  - Proliferative hippocampal activity in a group of patients with Rasmussen's 
      encephalitis: Neuronal, glial, and BDNF tissue expression correlations.
PG  - 29-37
LID - S1525-5050(17)30627-3 [pii]
LID - 10.1016/j.yebeh.2018.02.022 [doi]
AB  - Rasmussen's encephalitis (RE) is a rare and devastating unilateral inflammatory 
      brain disease that causes severe and intractable partial epilepsy. It has been 
      shown that epilepsy and subsequent inflammation have deleterious influence on 
      hippocampal cell survival and neurogenesis, but this still has not been 
      systematically explored in human tissue. In this study, we investigated the 
      correlation between inflammation and epilepsy as well as the rates of hippocampal 
      gliogenesis and neurogenesis in a pediatric group of six patients with RE and six 
      control cases. The dentate gyrus (DG) samples were obtained from patients who 
      underwent surgery for intractable RE. Sections were processed for 
      immunohistochemistry using antibodies against sex determining region Y-box 2 
      (Sox2), nestin, human protein encoded by MKI67 gen (Ki67), and brain-derived 
      neurotrophic factor (BDNF). There was an increase in the number of Ki67-positive 
      granule cells in the DG of patients with RE in comparison with the autopsy 
      control group, but no statistical difference for Sox2-positive cells was observed 
      between these groups. Nestin immunolabeling was less intense in the RE group 
      while BDNF expression was increased. Neurons that were BDNF-positive were found 
      in DG from patients with RE but not in the control group. In patients with RE, 
      few nestin-positive cells in DG were also positive for BDNF, unlike in controls 
      which showed no colocalization for these two markers. These results suggest a 
      proliferation activity in the DG subfield of patients with RE, and also future 
      studies are necessary to address the role of new cells in the hippocampus of 
      patients with RE.
CI  - Copyright (c) 2018 Elsevier Inc. All rights reserved.
FAU - Magno, Elane N
AU  - Magno EN
AD  - Laboratory of Experimental Neurology, Federal University of Sao Paulo, Brazil; 
      Laboratory of Histology, Federal University of Maranhao, Brazil. Electronic 
      address: elanemagno@gmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180323
PL  - United States
TA  - Epilepsy Behav
JT  - Epilepsy & behavior : E&B
JID - 100892858
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 7171WSG8A2 (BDNF protein, human)
SB  - IM
MH  - Brain-Derived Neurotrophic Factor/*biosynthesis/genetics
MH  - Case-Control Studies
MH  - Cell Proliferation/*physiology
MH  - Child
MH  - Child, Preschool
MH  - Dentate Gyrus/*metabolism/pathology/surgery
MH  - Encephalitis/*metabolism/pathology/surgery
MH  - Female
MH  - Gene Expression
MH  - Hippocampus/metabolism/pathology/surgery
MH  - Humans
MH  - Male
MH  - Neuroglia/*metabolism/pathology
MH  - Neurons/*metabolism/pathology
MH  - Tissue Distribution/physiology
OTO - NOTNLM
OT  - Epilepsy
OT  - Inflammation
OT  - Neurogenesis
OT  - Rasmussen's encephalitis
EDAT- 2018/03/27 06:00
MHDA- 2019/02/23 06:00
CRDT- 2018/03/27 06:00
PHST- 2017/07/25 00:00 [received]
PHST- 2018/02/21 00:00 [revised]
PHST- 2018/02/23 00:00 [accepted]
PHST- 2018/03/27 06:00 [pubmed]
PHST- 2019/02/23 06:00 [medline]
PHST- 2018/03/27 06:00 [entrez]
AID - S1525-5050(17)30627-3 [pii]
AID - 10.1016/j.yebeh.2018.02.022 [doi]
PST - ppublish
SO  - Epilepsy Behav. 2018 May;82:29-37. doi: 10.1016/j.yebeh.2018.02.022. Epub 2018 
      Mar 23.