PMID- 29580084
OWN - NLM
STAT- MEDLINE
DCOM- 20180831
LR  - 20180831
IS  - 0236-6290 (Print)
IS  - 0236-6290 (Linking)
VI  - 66
IP  - 1
DP  - 2018 Mar
TI  - Effect of methionine deficiency on oxidative stress and apoptosis in the small 
      intestine of broilers.
PG  - 52-65
LID - 10.1556/004.2018.006 [doi]
AB  - The aim of this study was to investigate the effects of methionine (Met) 
      deficiency on antioxidant functions (in the duodenal, jejunal and ileal mucosa) 
      and apoptosis in the duodenum, jejunum and ileum of broiler chickens. A total of 
      120 one-day-old Cobb broilers were divided into two groups and fed a Metdeficient 
      diet and a control diet, respectively, for six weeks. The activities of 
      superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px), 
      the ability to inhibit hydroxyl radicals, and glutathione (GSH) content were 
      significantly decreased in the Met-deficient group compared to the control. In 
      contrast, malondialdehyde (MDA) content was significantly higher in the 
      Met-deficient group. As measured by terminal deoxynucleotidyl transferase 
      2'-deoxyuridine 5'- triphosphate dUTP nick end-labelling (TUNEL) and flow 
      cytometry (FCM), the percentages of apoptotic cells were significantly increased. 
      In conclusion, dietary Met deficiency can cause oxidative stress and then induce 
      increased apoptosis in the intestine. Oxidative stress contributes to intestinal 
      apoptosis. This results in the impairment of local intestinal mucosal immunity 
      due to oxidative stress and apoptosis in the small intestine. The results of this 
      study provide new experimental evidence for understanding the negative effects of 
      Met deficiency on mucosal immunity or the functions of other immune tissues.
FAU - Ruan, Tao
AU  - Ruan T
AD  - 1 College of Life Science, China West Normal University , Shida road 1#, 
      Nanchong, Sichuan , PR China.
FAU - Li, Lingjun
AU  - Li L
AD  - 1 College of Life Science, China West Normal University , Shida road 1#, 
      Nanchong, Sichuan , PR China.
FAU - Lyu, Yingnan
AU  - Lyu Y
AD  - 4 Department of Microbiology and Immunology, National University of Singapore , 
      Singapore.
FAU - Luo, Qin
AU  - Luo Q
AD  - 3 College of Veterinary Medicine, Sichuan Agricultural University , Chengdu, 
      Sichuan , PR China.
FAU - Wu, Bangyuan
AU  - Wu B
AD  - 1 College of Life Science, China West Normal University , Shida road 1#, 
      Nanchong, Sichuan , PR China.
AD  - 2 Key Laboratory of Southwest China Wildlife Resources Conservation, Ministry of 
      Education , Nanchong, Sichuan , PR China.
LA  - eng
PT  - Controlled Clinical Trial
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Hungary
TA  - Acta Vet Hung
JT  - Acta veterinaria Hungarica
JID - 8406376
RN  - AE28F7PNPL (Methionine)
SB  - IM
MH  - Animal Feed/analysis
MH  - Animal Nutritional Physiological Phenomena
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - *Chickens
MH  - Diet/veterinary
MH  - Flow Cytometry/veterinary
MH  - In Situ Nick-End Labeling/veterinary
MH  - Intestine, Small/*drug effects/physiology
MH  - Methionine/administration & dosage/*deficiency
MH  - Oxidative Stress/*drug effects
OTO - NOTNLM
OT  - Methionine deficiency
OT  - apoptosis
OT  - broilers
OT  - oxidative stress
OT  - small intestine
EDAT- 2018/03/28 06:00
MHDA- 2018/09/01 06:00
CRDT- 2018/03/28 06:00
PHST- 2018/03/28 06:00 [entrez]
PHST- 2018/03/28 06:00 [pubmed]
PHST- 2018/09/01 06:00 [medline]
AID - 10.1556/004.2018.006 [doi]
PST - ppublish
SO  - Acta Vet Hung. 2018 Mar;66(1):52-65. doi: 10.1556/004.2018.006.