PMID- 29582602
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20180327
LR  - 20180327
IS  - 1872-2059 (Electronic)
IS  - 1000-8713 (Linking)
VI  - 36
IP  - 2
DP  - 2018 Feb 8
TI  - [Determination of metformin hydrochloride, melamine and dicyandiamide in 
      metformin hydrochloride preparations by tandem dual solid phase extraction 
      cartridges-high performance liquid chromatography-electrospray ionization 
      multi-stage mass spectrometry].
PG  - 159-166
LID - 10.3724/SP.J.1123.2017.10014 [doi]
AB  - A method for the confirmation and quantification of metformin hydrochloride and 
      its relative substances melamine and dicyandiamide using tandem dual solid phase 
      extraction (SPE) cartridges and high performance liquid 
      chromatography-electrospray ionization multi-stage mass spectrometry 
      (HPLC-ESI-MS(n)) was developed. The samples were extracted with anhydrous ethanol 
      containing 0.1% (v/v) acetic acid under ultrasound-assisted conditions. The 
      extracts were concentrated and purified using Cleanert PCX and C18 tandem dual 
      solid phase extraction cartridges, and eluted with 5% (v/v) ammonia methanol 
      solution. The separation was performed on a Kromasil-C18 column (100 mmx4.6 mm, 
      3.5 mum) with gradient elution. The detection was performed in selected ion 
      monitoring (SIM) mode using electrospray ionization multi-stage mass 
      spectrometry. The external standard method was used for quantification. The 
      extraction solvents, types of SPE cartridges and eluents were optimized by 
      comparing the recoveries under different conditions. The results showed that the 
      detector response of each target compound was linear in corresponding mass 
      concentration ranges with the correlation coefficients (r(2)) >/= 0.9992. The 
      limits of detection (LODs) and the limits of quantification (LOQs) of the three 
      analytes were 1.48-13.61 mug/kg and 5.96-45.67 mug/kg, respectively. The recoveries 
      of the three analytes were 65.02%-118.33% spiked at low, medium and high levels. 
      The relative standard deviations (RSDs) were no more than 13.41%. The method is 
      reliable, easy, and has a better purification effect. The method can be applied 
      to the routine analysis of metformin hydrochloride and its relative substances 
      melamine and dicyandiamide in different preparations of metformin hydrochloride.
FAU - Zhou, Yanfen
AU  - Zhou Y
AD  - School of Chemistry and Chemical Engineering, Ningxia University, State Key 
      Laboratory of High-efficiency Coal Utilization and Green Chemical Engineering, 
      Yinchuan 750021, China.
FAU - Wang, Fanghuan
AU  - Wang F
AD  - Inspection & Quarantine General Technical Center of Ningxia Entry-Exit Inspection 
      & Quarantine Bureau, Yinchuan 750001, China.
FAU - Wang, Zelan
AU  - Wang Z
AD  - School of Chemistry and Chemical Engineering, Ningxia University, State Key 
      Laboratory of High-efficiency Coal Utilization and Green Chemical Engineering, 
      Yinchuan 750021, China.
FAU - Zhan, Haijuan
AU  - Zhan H
AD  - School of Chemistry and Chemical Engineering, Ningxia University, State Key 
      Laboratory of High-efficiency Coal Utilization and Green Chemical Engineering, 
      Yinchuan 750021, China.
FAU - Liu, Wanyi
AU  - Liu W
AD  - School of Chemistry and Chemical Engineering, Ningxia University, State Key 
      Laboratory of High-efficiency Coal Utilization and Green Chemical Engineering, 
      Yinchuan 750021, China.
FAU - Meng, Zhe
AU  - Meng Z
AD  - School of Chemistry and Chemical Engineering, Ningxia University, State Key 
      Laboratory of High-efficiency Coal Utilization and Green Chemical Engineering, 
      Yinchuan 750021, China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
OTO - NOTNLM
OT  - dicyandiamide
OT  - high performance liquid chromatography-electrospray ionization multi-stage mass 
      spectrometry (HPLC-ESI-MSn)
OT  - melamine
OT  - metformin hydrochloride preparations
OT  - tandem dual solid phase extraction (SPE) cartridges
EDAT- 2018/03/28 06:00
MHDA- 2018/03/28 06:01
CRDT- 2018/03/28 06:00
PHST- 2018/03/28 06:00 [entrez]
PHST- 2018/03/28 06:00 [pubmed]
PHST- 2018/03/28 06:01 [medline]
AID - 10.3724/SP.J.1123.2017.10014 [doi]
PST - ppublish
SO  - Se Pu. 2018 Feb 8;36(2):159-166. doi: 10.3724/SP.J.1123.2017.10014.