PMID- 29582616
OWN - NLM
STAT- MEDLINE
DCOM- 20181004
LR  - 20181004
IS  - 1872-2059 (Electronic)
IS  - 1000-8713 (Linking)
VI  - 36
IP  - 1
DP  - 2018 Jan 8
TI  - [Determination of cyanamide residue in grapes and cherries by liquid 
      chromatography-tandem mass spectrometry coupled with precolumn derivatization].
PG  - 78-82
LID - 10.3724/SP.J.1123.2017.09004 [doi]
AB  - A method was developed for the quantitative determination of cyanamide in grapes 
      and cherries by liquid chromatography-tandem mass spectrometry (LC-MS/MS) coupled 
      with dansyl chloride (DNS) precolumn derivatization. First, the samples were 
      homogenized, and then extracted with ethyl acetate under ultrasonication. The 
      water was removed using anhydrous sodium sulfate, and the extract was 
      concentrated and derivatized with dansyl chloride under alkaline condition. The 
      separation was performed on a Shimadzu Shim-pack XR-ODS column (75 mmx2.0 mm, 1.6 
      mum) with the mobile phases of methanol and 2 mmol/L ammonium acetate aqueous 
      solution (containing 0.05% (v/v) formic acid) in a gradient elution mode. The 
      identification and quantification of cyanamide were carried out by MS/MS in 
      positive electrospray ionization (ESI(+)) and multiple reaction monitoring (MRM) 
      mode. The calibration curves showed good linearities in the range of 0.01-1.0 
      mg/L with the correlation coefficients not less than 0.9990. The average 
      recoveries of cyanamide spiked at 0.01, 0.05 and 1.0 mg/kg in cherries and grapes 
      were between 75% and 81%, and the relative standard deviations (RSDs) were 
      between 6.5% and 9.8%. Both the limits of quantification (LOQs) of the analytes 
      were 0.01 mg/kg. The method is simple, rapid, accurate and suitable for the 
      confirmation and quantification of cyanamide in cherries and grapes.
FAU - Lan, Feng
AU  - Lan F
AD  - Laboratory of Quality and Safety Risk Assessment for Fruit(Yantai), Ministry of 
      Agriculture, Yantai Academy of Agricultural Sciences in Shandong Province, Yantai 
      265500, China.
FAU - Liu, Chuande
AU  - Liu C
AD  - Laboratory of Quality and Safety Risk Assessment for Fruit(Yantai), Ministry of 
      Agriculture, Yantai Academy of Agricultural Sciences in Shandong Province, Yantai 
      265500, China. 343684502@qq.com.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Dansyl Compounds)
RN  - 420-04-2 (Cyanamide)
RN  - QMU9166TJ4 (dansyl chloride)
SB  - IM
MH  - *Chromatography, Liquid
MH  - Cyanamide/*analysis
MH  - Dansyl Compounds
MH  - *Food Analysis
MH  - *Fruit
MH  - Prunus
MH  - *Tandem Mass Spectrometry
MH  - Vitis
OTO - NOTNLM
OT  - cherry
OT  - cyanamide
OT  - dansyl chloride (DNS)
OT  - grape
OT  - liquid chromatography-tandem mass spectrometry (LC-MS/MS)
OT  - precolumn derivatization
EDAT- 2018/03/28 06:00
MHDA- 2018/10/05 06:00
CRDT- 2018/03/28 06:00
PHST- 2018/03/28 06:00 [entrez]
PHST- 2018/03/28 06:00 [pubmed]
PHST- 2018/10/05 06:00 [medline]
AID - 10.3724/SP.J.1123.2017.09004 [doi]
PST - ppublish
SO  - Se Pu. 2018 Jan 8;36(1):78-82. doi: 10.3724/SP.J.1123.2017.09004.