PMID- 29584650
OWN - NLM
STAT- MEDLINE
DCOM- 20190418
LR  - 20190418
IS  - 1999-4915 (Electronic)
IS  - 1999-4915 (Linking)
VI  - 10
IP  - 4
DP  - 2018 Mar 27
TI  - Identification of a Novel Recombinant Type 2 Porcine Reproductive and Respiratory 
      Syndrome Virus in China.
LID - 10.3390/v10040151 [doi]
LID - 151
AB  - Since the emergence of NADC30-like porcine reproductive and respiratory syndrome 
      virus (PRRSV) in China in 2013, PRRSVs have undergone rapid evolution. In this 
      study, a novel variant of PRRSV strain (designated SCcd17) was successfully 
      isolated from piglets with clinical signs in Sichuan Province in China in 2017, 
      and the complete genomic sequence was determined. The genome of this new isolate 
      was 15,015 nucleotides (nt) long, and comparative analysis revealed that SCcd17 
      exhibited 90.2%, 85.2%, 84.9%, and 84.0% nucleotide similarity to PRRSVs NADC30, 
      JXA1, CH-1a, and VR-2332, respectively. Phylogenetic analysis indicated that the 
      SCcd17 strain was classified into the NADC30-like sub-genotype, in which all the 
      strains contained the unique discontinuous 131-amino acid deletion in 
      nonstructural protein 2 (nsp2) when compared to VR-2332-like viruses. Notably, 
      extensive amino acid substitutions were observed in nsp2 and a unique single 
      amino acid deletion at position 33 of the GP5 is being described for the first 
      time. Strikingly, recombination analysis revealed that SCcd17 was the result of 
      recombination between the NADC30-like, JXA1-like, and VR-2332-like strains at 
      five recombination breakpoints: nsp1alpha (nt 641), nsp3 (nt 5141), nsp10 (nt 9521), 
      open reading frame 3 (ORF3) (nt 12,581), and ORF4 (nt 13,021). The genomic data 
      of SCcd17 will be helpful for understanding the role of genomic recombination in 
      the evolution of PRRSV.
FAU - Zhou, Long
AU  - Zhou L
AD  - School of Life Science, Sichuan University, Animal Disease Prevention and Food 
      Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-Resources and 
      Eco-Environment, Ministry of Education, 29# Wangjiang Road, Chengdu 610064, 
      China. 2014322040041@stu.scu.edu.cn.
FAU - Kang, Runmin
AU  - Kang R
AD  - Sichuan Animal Science Academy, Sichuan Provincial Key laboratory of Animal 
      Breeding and Genetics, Chengdu 610066, China. angelina_0708@hotmail.com.
FAU - Xie, Bo
AU  - Xie B
AD  - Chengdu Chia Tai Agro-Industry & Food Co., Ltd., Animal Healthy Disease Service, 
      Gongping Town, Wenjiang District, Chengdu 610081, China. bobosky123@163.com.
FAU - Tian, Yiming
AU  - Tian Y
AD  - School of Life Science, Sichuan University, Animal Disease Prevention and Food 
      Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-Resources and 
      Eco-Environment, Ministry of Education, 29# Wangjiang Road, Chengdu 610064, 
      China. 2015322040022@stu.scu.edu.cn.
FAU - Wu, Xuan
AU  - Wu X
AD  - School of Life Science, Sichuan University, Animal Disease Prevention and Food 
      Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-Resources and 
      Eco-Environment, Ministry of Education, 29# Wangjiang Road, Chengdu 610064, 
      China. 2016322040030@stu.scu.edu.cn.
FAU - Lv, Xuebin
AU  - Lv X
AD  - Sichuan Animal Science Academy, Sichuan Provincial Key laboratory of Animal 
      Breeding and Genetics, Chengdu 610066, China. lvxuebin@21cn.com.
FAU - Yang, Xin
AU  - Yang X
AD  - School of Life Science, Sichuan University, Animal Disease Prevention and Food 
      Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-Resources and 
      Eco-Environment, Ministry of Education, 29# Wangjiang Road, Chengdu 610064, 
      China. xinyang@scu.edu.cn.
FAU - Wang, Hongning
AU  - Wang H
AD  - School of Life Science, Sichuan University, Animal Disease Prevention and Food 
      Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-Resources and 
      Eco-Environment, Ministry of Education, 29# Wangjiang Road, Chengdu 610064, 
      China. whongning@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180327
PL  - Switzerland
TA  - Viruses
JT  - Viruses
JID - 101509722
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Nonstructural Proteins)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Amino Acid Sequence
MH  - Amino Acid Substitution
MH  - Animals
MH  - China
MH  - Evolution, Molecular
MH  - Genetic Variation
MH  - Genome, Viral/genetics
MH  - Genomics
MH  - *Phylogeny
MH  - Porcine respiratory and reproductive syndrome virus/*classification/*genetics
MH  - *Recombination, Genetic
MH  - Sequence Alignment/veterinary
MH  - Sequence Deletion
MH  - Sequence Homology, Amino Acid
MH  - Swine
MH  - Viral Envelope Proteins/genetics
MH  - Viral Nonstructural Proteins/genetics
PMC - PMC5923445
OTO - NOTNLM
OT  - phylogenetic analysis
OT  - porcine reproductive and respiratory syndrome virus
OT  - recombination
OT  - sub-genotype
COIS- The authors declare no conflict of interest.
EDAT- 2018/03/28 06:00
MHDA- 2019/04/19 06:00
PMCR- 2018/04/01
CRDT- 2018/03/28 06:00
PHST- 2018/02/18 00:00 [received]
PHST- 2018/03/20 00:00 [revised]
PHST- 2018/03/22 00:00 [accepted]
PHST- 2018/03/28 06:00 [entrez]
PHST- 2018/03/28 06:00 [pubmed]
PHST- 2019/04/19 06:00 [medline]
PHST- 2018/04/01 00:00 [pmc-release]
AID - v10040151 [pii]
AID - viruses-10-00151 [pii]
AID - 10.3390/v10040151 [doi]
PST - epublish
SO  - Viruses. 2018 Mar 27;10(4):151. doi: 10.3390/v10040151.