PMID- 2958544
OWN - NLM
STAT- MEDLINE
DCOM- 19871104
LR  - 20161019
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 139
IP  - 7
DP  - 1987 Oct 1
TI  - Effect of B cell stimulatory factor-1 (interleukin 4) on Fc epsilon and Fc gamma 
      receptor expression on murine B lymphocytes and B cell lines.
PG  - 2290-6
AB  - Culture of murine splenic B cells with interleukin 4 (IL-4) caused the 
      up-regulation of the lymphocyte Fc receptor for immunoglobulin E (IgE) (Fc 
      epsilon R) over a similar dose range as required for Ia up-regulation. However, 
      the expression level of the Fc receptor for immunoglobulin G (Fc gamma R) did not 
      increase, rather IL-4 caused a slight but consistent decrease in the Fc gamma R 
      level on the B cells. Fc epsilon R+ B hybridoma cells also responded to IL-4 by 
      exhibiting increased Fc epsilon R expression; with the hybridoma cells Fc gamma R 
      levels were unaffected. IL-4 caused an increase in the number of Fc epsilon R per 
      cell and the highest levels of expression were obtained by having both IgE and 
      IL-4 present in the culture. The specificity of the increase was demonstrated by 
      blocking IL-4-mediated actions with monoclonal anti-IL-4 (11B11). Experiments 
      following the incorporation of [35S]methionine into the Fc epsilon R demonstrated 
      that IL-4 increased the rate of Fc epsilon R biosynthesis; this provides an 
      explanation for the IL-4-induced increase in Fc epsilon R expression. IL-4, 
      unlike IgE, had no effect on the rate of degradation of the Fc epsilon R. 
      Interferon-gamma (IFN-gamma) totally abrogated IL-4-mediated Fc epsilon R 
      up-regulation; at the same concentration of IFN-gamma Ia up-regulation is also 
      suppressed, although not as effectively. IFN-gamma was shown to directly suppress 
      Fc epsilon R synthesis, thereby explaining the inhibitory action on Fc epsilon R 
      levels. Finally, it was shown that 11B11 inhibited the increased expression of Fc 
      epsilon R on B cells obtained from mice during the early, but not the late, 
      stages of Nippostrongylus brasiliensis infection. This latter finding suggests 
      that the high Fc epsilon R levels seen early in parasite infections are dependent 
      upon IL-4. The results overall provide further insight into the biologic 
      activities of IL-4.
FAU - Conrad, D H
AU  - Conrad DH
AD  - Subdepartment of Immunology, Johns Hopkins University School of Medicine, 
      Baltimore, MD 21205.
FAU - Waldschmidt, T J
AU  - Waldschmidt TJ
FAU - Lee, W T
AU  - Lee WT
FAU - Rao, M
AU  - Rao M
FAU - Keegan, A D
AU  - Keegan AD
FAU - Noelle, R J
AU  - Noelle RJ
FAU - Lynch, R G
AU  - Lynch RG
FAU - Kehry, M R
AU  - Kehry MR
LA  - eng
GR  - R01 AI018697/AI/NIAID NIH HHS/United States
GR  - AI 18697/AI/NIAID NIH HHS/United States
GR  - AI 22600/AI/NIAID NIH HHS/United States
GR  - CA 32277/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Interleukins)
RN  - 0 (Receptors, Fc)
RN  - 0 (Receptors, IgE)
RN  - 0 (Receptors, IgG)
RN  - 207137-56-2 (Interleukin-4)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - B-Lymphocytes/*drug effects/immunology
MH  - Cell Line
MH  - Gene Expression Regulation/drug effects
MH  - Hybridomas/drug effects/immunology
MH  - Interferon-gamma/pharmacology
MH  - Interleukin-4
MH  - Interleukins/antagonists & inhibitors/*pharmacology
MH  - Mice
MH  - Nematode Infections/immunology
MH  - Nippostrongylus
MH  - Receptors, Fc/*biosynthesis
MH  - Receptors, IgE
MH  - Receptors, IgG
EDAT- 1987/10/01 00:00
MHDA- 1987/10/01 00:01
CRDT- 1987/10/01 00:00
PHST- 1987/10/01 00:00 [pubmed]
PHST- 1987/10/01 00:01 [medline]
PHST- 1987/10/01 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1987 Oct 1;139(7):2290-6.