PMID- 29587834
OWN - NLM
STAT- MEDLINE
DCOM- 20180925
LR  - 20181114
IS  - 1756-3305 (Electronic)
IS  - 1756-3305 (Linking)
VI  - 11
IP  - 1
DP  - 2018 Mar 27
TI  - A standard photomap of ovarian nurse cell chromosomes and inversion polymorphism 
      in Anopheles beklemishevi.
PG  - 211
LID - 10.1186/s13071-018-2657-3 [doi]
LID - 211
AB  - BACKGROUND: Anopheles beklemishevi is a member of the Maculipennis group of 
      malaria mosquitoes that has the most northern distribution among other members of 
      the group. Although a cytogenetic map for the larval salivary gland chromosomes 
      of this species has been developed, a high-quality standard cytogenetic photomap 
      that enables genomics and population genetics studies of this mosquito at the 
      adult stage is still lacking. METHODS: In this study, a cytogenetic map for the 
      polytene chromosomes of An. beklemishevi from ovarian nurse cells was developed 
      using high-resolution digital imaging from field collected mosquitoes. 
      PCR-amplified DNA probes for fluorescence in situ hybridization (FISH) were 
      designed based on the genome of An. atroparvus. The DNA probe obtained by 
      microdissection procedures from the breakpoint region was labelled in a DOP-PCR 
      reaction. Population analysis was performed on 371 specimens collected in 18 
      locations. RESULTS: We report the development of a high-quality standard photomap 
      for the polytene chromosomes from ovarian nurse cells of An. beklemishevi. To 
      confirm the suitability of the map for physical mapping, several PCR-amplified 
      probes were mapped to the chromosomes of An. beklemishevi using FISH. In 
      addition, we identified and mapped DNA probes to flanking regions of the 
      breakpoints of two inversions on chromosome X of this species. Inversion 
      polymorphism was determined in 13 geographically distant populations of An. 
      beklemishevi. Four polymorphic inversions were detected. The positions of common 
      chromosomal inversions were indicated on the map. CONCLUSIONS: The study 
      constructed a standard photomap for ovarian nurse cell chromosomes of An. 
      beklemishevi and tested its suitability for physical genome mapping and 
      population studies. Cytogenetic analysis determined inversion polymorphism in 
      natural populations of An. beklemishevi related to this species' adaptation.
FAU - Artemov, Gleb N
AU  - Artemov GN
AD  - Laboratory of Ecology, Genetics and Environmental Protection, Tomsk State 
      University, Tomsk, Russia.
FAU - Gordeev, Mikhail I
AU  - Gordeev MI
AD  - Department of General Biology and Ecology, Moscow Regional State University, 
      Moscow, Russia.
FAU - Kokhanenko, Alina A
AU  - Kokhanenko AA
AD  - Laboratory of Ecology, Genetics and Environmental Protection, Tomsk State 
      University, Tomsk, Russia.
FAU - Moskaev, Anton V
AU  - Moskaev AV
AD  - Department of General Biology and Ecology, Moscow Regional State University, 
      Moscow, Russia.
FAU - Velichevskaya, Alena I
AU  - Velichevskaya AI
AD  - Laboratory of Ecology, Genetics and Environmental Protection, Tomsk State 
      University, Tomsk, Russia.
FAU - Stegniy, Vladimir N
AU  - Stegniy VN
AD  - Laboratory of Ecology, Genetics and Environmental Protection, Tomsk State 
      University, Tomsk, Russia.
FAU - Sharakhov, Igor V
AU  - Sharakhov IV
AD  - Laboratory of Ecology, Genetics and Environmental Protection, Tomsk State 
      University, Tomsk, Russia. igor@vt.edu.
AD  - Virginia Tech, Department of Entomology, Fralin Life Science Institute, 
      Blacksburg, VA, USA. igor@vt.edu.
FAU - Sharakhova, Maria V
AU  - Sharakhova MV
AD  - Laboratory of Ecology, Genetics and Environmental Protection, Tomsk State 
      University, Tomsk, Russia. msharakh@vt.edu.
AD  - Virginia Tech, Department of Entomology, Fralin Life Science Institute, 
      Blacksburg, VA, USA. msharakh@vt.edu.
LA  - eng
GR  - 15-14-20011/Russian Science Foundation/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180327
PL  - England
TA  - Parasit Vectors
JT  - Parasites & vectors
JID - 101462774
SB  - IM
MH  - Animals
MH  - Anopheles/*cytology/*genetics
MH  - *Chromosome Inversion
MH  - *Chromosomes, Insect
MH  - Cytogenetics/*methods
MH  - Female
MH  - Genetics, Population
MH  - In Situ Hybridization, Fluorescence
MH  - Ovary/cytology
MH  - Physical Chromosome Mapping
MH  - *Polymorphism, Genetic
MH  - *Polytene Chromosomes
PMC - PMC5870207
OTO - NOTNLM
OT  - Anopheles
OT  - Cytogenetic map
OT  - Inversion polymorphism
OT  - Mosquito
OT  - Physical mapping
COIS- ETHICS APPROVAL AND CONSENT TO PARTICIPATE: Not applicable. CONSENT FOR 
      PUBLICATION: Not applicable. COMPETING INTERESTS: The authors declare that they 
      have no competing interests. PUBLISHER'S NOTE: Springer Nature remains neutral 
      with regard to jurisdictional claims in published maps and institutional 
      affiliations.
EDAT- 2018/03/29 06:00
MHDA- 2018/09/27 06:00
PMCR- 2018/03/27
CRDT- 2018/03/29 06:00
PHST- 2017/09/19 00:00 [received]
PHST- 2018/01/17 00:00 [accepted]
PHST- 2018/03/29 06:00 [entrez]
PHST- 2018/03/29 06:00 [pubmed]
PHST- 2018/09/27 06:00 [medline]
PHST- 2018/03/27 00:00 [pmc-release]
AID - 10.1186/s13071-018-2657-3 [pii]
AID - 2657 [pii]
AID - 10.1186/s13071-018-2657-3 [doi]
PST - epublish
SO  - Parasit Vectors. 2018 Mar 27;11(1):211. doi: 10.1186/s13071-018-2657-3.