PMID- 29589579
OWN - NLM
STAT- MEDLINE
DCOM- 20190920
LR  - 20220915
IS  - 1560-7917 (Electronic)
IS  - 1025-496X (Print)
IS  - 1025-496X (Linking)
VI  - 23
IP  - 12
DP  - 2018 Mar
TI  - Field investigation with real-time virus genetic characterisation support of a 
      cluster of Ebola virus disease cases in Dubreka, Guinea, April to June 2015.
LID - 10.2807/1560-7917.ES.2018.23.12.17-00140 [doi]
LID - 17-00140
AB  - On 11 May 2015, the Dubreka prefecture, Guinea, reported nine 
      laboratory-confirmed cases of Ebola virus disease (EVD). None could be 
      epidemiologically linked to cases previously reported in the prefecture. We 
      describe the epidemiological and molecular investigations of this event. We used 
      the Dubreka EVD registers and the Ebola treatment centre's (ETC) records to 
      characterise chains of transmission. Real-time field Ebola virus sequencing was 
      employed to support epidemiological results. An epidemiological cluster of 32 
      cases was found, of which 27 were laboratory confirmed, 24 were isolated and 20 
      died. Real-time viral sequencing on 12 cases demonstrated SL3 lineage viruses 
      with sequences differing by one to three nt inside a single phylogenetic cluster. 
      For isolated cases, the average time between symptom onset and ETC referral was 
      2.8 days (interquartile range (IQR): 1-4). The average time between sample 
      collection and molecular results' availability was 3 days (IQR: 2-5). In an area 
      with scarce resources, the genetic characterisation supported the outbreak 
      investigations in real time, linking cases where epidemiological investigation 
      was limited and reassuring that the responsible strain was already circulating in 
      Guinea. We recommend coupling thorough epidemiological and genomic investigations 
      to control EVD clusters.
FAU - Pini, Alessandro
AU  - Pini A
AD  - European Centre for Disease Prevention and Control (ECDC), Solna, Sweden.
AD  - The Public Health Agency of Sweden, Solna, Sweden.
FAU - Zomahoun, Delayo
AU  - Zomahoun D
AD  - Centers for Disease Control and Prevention, Atlanta, Georgia, United States.
FAU - Duraffour, Sophie
AU  - Duraffour S
AD  - Bernhard-Nocht-Institute for Tropical Medicine, Hamburg, Germany.
AD  - The European Mobile Laboratory Consortium, Bernhard-Nocht-Institute for Tropical 
      Medicine, Hamburg, Germany.
FAU - Derrough, Tarik
AU  - Derrough T
AD  - European Centre for Disease Prevention and Control (ECDC), Solna, Sweden.
FAU - Charles, Myrna
AU  - Charles M
AD  - Centers for Disease Control and Prevention, Atlanta, Georgia, United States.
FAU - Quick, Joshua
AU  - Quick J
AD  - Institute of Microbiology and Infection, University of Birmingham, Birmingham, 
      United Kingdom.
FAU - Loman, Nick
AU  - Loman N
AD  - Institute of Microbiology and Infection, University of Birmingham, Birmingham, 
      United Kingdom.
FAU - Cowley, Lauren
AU  - Cowley L
AD  - Gastrointestinal Bacterial Reference Unit, Colindale, Public Health England, 
      London, United Kingdom.
FAU - Leno, Mamadou
AU  - Leno M
AD  - Direction Prefectural de la Sante, Dubreka, Guinea.
FAU - Ouedraogo, Nobila
AU  - Ouedraogo N
AD  - Postgraduate Training for Applied Epidemiology, Robert Koch Institute, Berlin, 
      Germany.
FAU - Thiam, Oumou
AU  - Thiam O
AD  - World Health Organization, Conakry, Guinea.
FAU - Hernandez-Romieu, Alfonso
AU  - Hernandez-Romieu A
AD  - Centers for Disease Control and Prevention, Atlanta, Georgia, United States.
FAU - Iko, Annie
AU  - Iko A
AD  - Centers for Disease Control and Prevention, Atlanta, Georgia, United States.
FAU - Keita, Halimatou
AU  - Keita H
AD  - World Health Organization, Conakry, Guinea.
FAU - Konate, Djiba
AU  - Konate D
AD  - World Health Organization, Conakry, Guinea.
FAU - Soumah, Aboubacar Aboubak
AU  - Soumah AA
AD  - World Health Organization, Conakry, Guinea.
FAU - Bouchouar, Etran
AU  - Bouchouar E
AD  - Centers for Disease Control and Prevention, Atlanta, Georgia, United States.
FAU - Ileka-Priouzeau, Samuel
AU  - Ileka-Priouzeau S
AD  - Centers for Disease Control and Prevention, Atlanta, Georgia, United States.
FAU - Keita, Sakoba
AU  - Keita S
AD  - Ministere de la Sante, Conakry, Guinea.
FAU - Diallo, Boubacar
AU  - Diallo B
AD  - World Health Organization, Conakry, Guinea.
FAU - Cisse, Fode
AU  - Cisse F
AD  - Direction Prefectural de la Sante, Dubreka, Guinea.
FAU - Jansa, Josep
AU  - Jansa J
AD  - European Centre for Disease Prevention and Control (ECDC), Solna, Sweden.
FAU - Carroll, Miles
AU  - Carroll M
AD  - National Infection Service, Public Health England, Porton Down, Wiltshire, United 
      Kingdom.
AD  - University of Southampton, South General Hospital, Southampton, United Kingdom.
FAU - Gunther, Stephan
AU  - Gunther S
AD  - Bernhard-Nocht-Institute for Tropical Medicine, Hamburg, Germany.
AD  - The European Mobile Laboratory Consortium, Bernhard-Nocht-Institute for Tropical 
      Medicine, Hamburg, Germany.
FAU - Severi, Ettore
AU  - Severi E
AD  - European Centre for Disease Prevention and Control (ECDC), Solna, Sweden.
FAU - Formenty, Pierre
AU  - Formenty P
AD  - World Health Organization, Geneva, Switzerland.
LA  - eng
GR  - 001/WHO_/World Health Organization/International
GR  - MR/L015080/1/MRC_/Medical Research Council/United Kingdom
GR  - MR/M501621/1/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Sweden
TA  - Euro Surveill
JT  - Euro surveillance : bulletin Europeen sur les maladies transmissibles = European 
      communicable disease bulletin
JID - 100887452
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Contact Tracing
MH  - DNA, Viral/*genetics
MH  - Disease Outbreaks/prevention & control
MH  - Ebolavirus/*genetics/*isolation & purification
MH  - Genomics
MH  - Guinea/epidemiology
MH  - Hemorrhagic Fever, Ebola/*diagnosis/epidemiology/*transmission/virology
MH  - Humans
MH  - Phylogeny
MH  - Real-Time Polymerase Chain Reaction
PMC - PMC6205262
OTO - NOTNLM
OT  - Ebola virus
OT  - Guinea
OT  - laboratory surveillance
OT  - outbreaks
OT  - viral haemorrhagic fever
COIS- Conflict of interest: None declared.
EDAT- 2018/03/29 06:00
MHDA- 2019/09/21 06:00
PMCR- 2018/03/22
CRDT- 2018/03/29 06:00
PHST- 2018/03/29 06:00 [entrez]
PHST- 2018/03/29 06:00 [pubmed]
PHST- 2019/09/21 06:00 [medline]
PHST- 2018/03/22 00:00 [pmc-release]
AID - 17-00140 [pii]
AID - 10.2807/1560-7917.ES.2018.23.12.17-00140 [doi]
PST - ppublish
SO  - Euro Surveill. 2018 Mar;23(12):17-00140. doi: 
      10.2807/1560-7917.ES.2018.23.12.17-00140.