PMID- 29590659 OWN - NLM STAT- MEDLINE DCOM- 20180625 LR - 20220408 IS - 1421-9778 (Electronic) IS - 1015-8987 (Linking) VI - 46 IP - 1 DP - 2018 TI - LncRNA-AK131850 Sponges MiR-93-5p in Newborn and Mature Osteoclasts to Enhance the Secretion of Vascular Endothelial Growth Factor a Promoting Vasculogenesis of Endothelial Progenitor Cells. PG - 401-417 LID - 10.1159/000488474 [doi] AB - BACKGROUND/AIMS: In the process of bone development and remodeling, the vasculature is regarded as the communicative network between the bone and neighboring tissues. Recently, it has been reported that the processes of angiogenesis and osteogenesis are coupled temporally and spatially. However, few studies reported the relationship and relevant mechanism between osteoclastogenesis and vasculogenesis. METHODS: Arraystar Mouse lncRNA microarray V3.0 was firstly used to analyze the differentially expressed lncRNA genes in osteoclast different stages during osteoclastogenesis. Cell counting kit 8 (CCK-8) analysis, quantitative real-time polymerase chain reaction (qRT-PCR) analysis, migration and tube formation assays were used to detect impact of osteoclast different stages on the proliferation, differentiation, migration and tube formation of endothelial progenitor cells (EPCs), respectively. Finally, transfection of AK131850 shRNA, miR-93-5p mimic and miR-93-5p inhibitor, qRT-PCR, western blotting, enzyme-linked immunosorbent assay (ELISA), fluorescence in situ hybridization (FISH) and luciferase reporter assay were carried out to dissect molecular mechanisms. RESULTS: In this study, we found that newborn OCs (N-OC) and mature OCs (M-OC) during osteoclastogenesis significantly promoted proliferation, differentiation, migration and tube formation of endothelial progenitor cells (EPCs). Through lncRNA microarray and GO&pathway analysis, we found that AK131850 and co-expressed gene, vascular endothelial growth factor a (VEGFa), were significantly up-regulated in N-OC and M-OC. After inhibition of AK131850 the promoting effect of N-OC and M-OC on EPCs was reversed. Furthermore, we found that AK131850 directly competed miR-93-5p in N-OC and M-OC through sponge, thereby increasing VEGFa transcription, expression and secretion through derepressing of miR-93-5p on VEGFa. CONCLUSION: Our results provided the first finding that lncRNA-AK131850 sponged miR-93-5p in N-OC and M-OC during osteoclastogenesis to enhance the secretion of VEGFa, thus promoting vasculogenesis of EPCs. CI - (c) 2018 The Author(s). Published by S. Karger AG, Basel. FAU - Quan, Hongyu AU - Quan H AD - Department of Biomedical Materials Science, School of Biomedical Engineering, Third Military Medical University, Chongqing, China. AD - State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University, Chongqing, China. FAU - Liang, Mengmeng AU - Liang M AD - Department of Biomedical Materials Science, School of Biomedical Engineering, Third Military Medical University, Chongqing, China. FAU - Li, Nan AU - Li N AD - Department of Biomedical Materials Science, School of Biomedical Engineering, Third Military Medical University, Chongqing, China. FAU - Dou, Ce AU - Dou C AD - Department of Biomedical Materials Science, School of Biomedical Engineering, Third Military Medical University, Chongqing, China. FAU - Liu, Chuan AU - Liu C AD - Department of Biomedical Materials Science, School of Biomedical Engineering, Third Military Medical University, Chongqing, China. FAU - Bai, Yun AU - Bai Y AD - Department of Biomedical Materials Science, School of Biomedical Engineering, Third Military Medical University, Chongqing, China. FAU - Luo, Wei AU - Luo W AD - Department of Orthopedics, Guizhou Provincial People's Hospital, Guiyang, China. FAU - Li, Jianmei AU - Li J AD - Department of Biomedical Materials Science, School of Biomedical Engineering, Third Military Medical University, Chongqing, China. FAU - Kang, Fei AU - Kang F AD - Department of Biomedical Materials Science, School of Biomedical Engineering, Third Military Medical University, Chongqing, China. FAU - Cao, Zhen AU - Cao Z AD - Department of Biomedical Materials Science, School of Biomedical Engineering, Third Military Medical University, Chongqing, China. FAU - Yang, Xiaochao AU - Yang X AD - Department of Biomedical Materials Science, School of Biomedical Engineering, Third Military Medical University, Chongqing, China. FAU - Jiang, Hong AU - Jiang H AD - Department of Biomedical Materials Science, School of Biomedical Engineering, Third Military Medical University, Chongqing, China. FAU - Dong, Shiwu AU - Dong S AD - Department of Biomedical Materials Science, School of Biomedical Engineering, Third Military Medical University, Chongqing, China. AD - State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University, Chongqing, China. LA - eng PT - Journal Article DEP - 20180323 PL - Germany TA - Cell Physiol Biochem JT - Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology JID - 9113221 RN - 0 (Culture Media, Conditioned) RN - 0 (MicroRNAs) RN - 0 (Platelet Endothelial Cell Adhesion Molecule-1) RN - 0 (RNA, Long Noncoding) RN - 0 (Vascular Endothelial Growth Factor A) RN - EC 2.7.10.1 (Kdr protein, mouse) RN - EC 2.7.10.1 (Vascular Endothelial Growth Factor Receptor-2) SB - IM EIN - Cell Physiol Biochem. 2021;55(1):135-136. PMID: 34037341 MH - Animals MH - Base Sequence MH - Cell Differentiation MH - Cell Movement MH - Cell Proliferation MH - Cells, Cultured MH - Culture Media, Conditioned/pharmacology MH - Endothelial Progenitor Cells/cytology/metabolism MH - Male MH - Mice MH - Mice, Inbred C57BL MH - MicroRNAs/antagonists & inhibitors/genetics/*metabolism MH - Oligonucleotide Array Sequence Analysis MH - Osteoclasts/cytology/metabolism MH - Platelet Endothelial Cell Adhesion Molecule-1/genetics/metabolism MH - RNA Interference MH - RNA, Long Noncoding/antagonists & inhibitors/genetics/*metabolism MH - Sequence Alignment MH - Vascular Endothelial Growth Factor A/*metabolism MH - Vascular Endothelial Growth Factor Receptor-2/genetics/metabolism OTO - NOTNLM OT - AK131850 OT - Endothelial progenitor cells OT - MiR-93-5p OT - Osteoclast OT - VEGFa OT - Vasculogenesis EDAT- 2018/03/29 06:00 MHDA- 2018/06/26 06:00 CRDT- 2018/03/29 06:00 PHST- 2017/11/23 00:00 [received] PHST- 2018/02/27 00:00 [accepted] PHST- 2018/03/29 06:00 [pubmed] PHST- 2018/06/26 06:00 [medline] PHST- 2018/03/29 06:00 [entrez] AID - 000488474 [pii] AID - 10.1159/000488474 [doi] PST - ppublish SO - Cell Physiol Biochem. 2018;46(1):401-417. doi: 10.1159/000488474. Epub 2018 Mar 23.