PMID- 2959318
OWN - NLM
STAT- MEDLINE
DCOM- 19871207
LR  - 20190613
IS  - 0006-2960 (Print)
IS  - 0006-2960 (Linking)
VI  - 26
IP  - 15
DP  - 1987 Jul 28
TI  - A cDNA presumptively coding for the alpha subunit of the receptor with high 
      affinity for immunoglobulin E.
PG  - 4605-10
AB  - Rat mast cells and a neoplastic analogue such as rat basophilic leukemia (RBL) 
      cells have receptors that have exceptionally high affinity for immunoglobulin E 
      (IgE). When aggregated, these receptors induce cellular degranulation. The alpha 
      chain of the receptor contains the binding site for IgE; the function(s) of the 
      noncovalently associated beta and gamma chains is (are) still undefined. Using a 
      cDNA library constructed from the mRNA of RBL cells, we have isolated a cDNA 
      clone whose sequence predicts a putative 23-residue signal peptide, followed by a 
      sequence that accurately predicts the amino acid composition, the peptide 
      molecular weight, and six peptide sequences (encompassing 59 residues or 26% of 
      the total number) determined for the alpha chain by direct analysis. These 
      findings provide strong evidence that the cDNA codes for the alpha chain, even 
      though expression has not been unambiguously achieved. The sequence suggests that 
      the alpha chain contains a 180-residue extracellular portion with two homologous 
      domains of approximately 35 residues, a 20-residue transmembrane segment 
      containing an aspartic acid, and a 27-residue cytoplasmic portion containing 9 
      basic amino acids. The sequence shows no homology with the low-affinity receptor 
      for IgE from lymphocytes but over 30% homology with an Fc gamma receptor.
FAU - Kinet, J P
AU  - Kinet JP
AD  - Section on Chemical Immunology, National Institute of Arthritis and 
      Musculoskeletal and Skin Diseases, Bethesda, Maryland 20892.
FAU - Metzger, H
AU  - Metzger H
FAU - Hakimi, J
AU  - Hakimi J
FAU - Kochan, J
AU  - Kochan J
LA  - eng
SI  - GENBANK/M17153
PT  - Journal Article
PL  - United States
TA  - Biochemistry
JT  - Biochemistry
JID - 0370623
RN  - 0 (Macromolecular Substances)
RN  - 0 (Receptors, Fc)
RN  - 0 (Receptors, IgE)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9007-49-2 (DNA)
SB  - IM
EIN - Biochemistry 1988 Nov 15;27(23):8694
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Cloning, Molecular
MH  - DNA/*metabolism
MH  - Escherichia coli/genetics
MH  - Immunoglobulin E
MH  - Macromolecular Substances
MH  - Receptors, Fc/*genetics
MH  - Receptors, IgE
EDAT- 1987/07/28 00:00
MHDA- 1987/07/28 00:01
CRDT- 1987/07/28 00:00
PHST- 1987/07/28 00:00 [pubmed]
PHST- 1987/07/28 00:01 [medline]
PHST- 1987/07/28 00:00 [entrez]
AID - 10.1021/bi00389a002 [doi]
PST - ppublish
SO  - Biochemistry. 1987 Jul 28;26(15):4605-10. doi: 10.1021/bi00389a002.