PMID- 29593719 OWN - NLM STAT- MEDLINE DCOM- 20190624 LR - 20190624 IS - 1664-3224 (Print) IS - 1664-3224 (Electronic) IS - 1664-3224 (Linking) VI - 9 DP - 2018 TI - Human Milk Oligosaccharide 2'-Fucosyllactose Improves Innate and Adaptive Immunity in an Influenza-Specific Murine Vaccination Model. PG - 452 LID - 10.3389/fimmu.2018.00452 [doi] LID - 452 AB - BACKGROUND: Human milk is uniquely suited to provide optimal nutrition and immune protection to infants. Human milk oligosaccharides are structural complex and diverse consisting of short chain and long chain oligosaccharides typically present in a 9:1 ratio. 2'-Fucosyllactose (2'FL) is one of the most prominent short chain oligosaccharides and is associated with anti-infective capacity of human milk. AIM: To determine the effect of 2'FL on vaccination responsiveness (both innate and adaptive) in a murine influenza vaccination model and elucidate mechanisms involved. METHODS: A dose range of 0.25-5% (w/w) dietary 2'FL was provided to 6-week-old female C57Bl/6JOlaHsd mice 2 weeks prior primary and booster vaccination until the end of the experiment. Intradermal (i.d.) challenge was performed to measure the vaccine-specific delayed-type hypersensitivity (DTH). Antigen-specific antibody levels in serum as well as immune cell populations within several organs were evaluated using ELISA and flow cytometry, respectively. In an ex vivo restimulation assay, spleen cells were cocultured with influenza-loaded bone marrow-derived dendritic cells (BMDCs) to study the effects of 2'FL on vaccine-specific CD4+ and CD8+ T-cell proliferation and cytokine secretions. Furthermore, the direct immune regulatory effects of 2'FL were confirmed using in vitro BMDCs T-cell cocultures. RESULTS: Dietary 2'FL significantly (p < 0.05) enhanced vaccine specific DTH responses accompanied by increased serum levels of vaccine-specific immunoglobulin (Ig) G1 and IgG2a in a dose-dependent manner. Consistently, increased activation marker (CD27) expression on splenic B-cells was detected in mice receiving 2'FL as compared to control mice. Moreover, proliferation of vaccine-specific CD4+ and CD8+ T-cells, as well as interferon-gamma production after ex vivo restimulation were significantly increased in spleen cells of mice receiving 2'FL as compared to control mice, which were in line with changes detected within dendritic cell populations. Finally, we confirmed a direct effect of 2'FL on the maturation status and antigen presenting capacity of BMDCs. CONCLUSION: Dietary intervention with 2'FL improves both humoral and cellular immune responses to vaccination in mice, which might be attributed in part to the direct effects of 2'FL on immune cell differentiation. FAU - Xiao, Ling AU - Xiao L AD - Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, Utrecht, Netherlands. FAU - Leusink-Muis, Thea AU - Leusink-Muis T AD - Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, Utrecht, Netherlands. FAU - Kettelarij, Nienke AU - Kettelarij N AD - Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, Utrecht, Netherlands. AD - Department of Immunology, Human Milk Research, Nutricia Research, Utrecht, Netherlands. FAU - van Ark, Ingrid AU - van Ark I AD - Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, Utrecht, Netherlands. FAU - Blijenberg, Bernadet AU - Blijenberg B AD - Department of Immunology, Human Milk Research, Nutricia Research, Utrecht, Netherlands. FAU - Hesen, Nienke A AU - Hesen NA AD - Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, Utrecht, Netherlands. FAU - Stahl, Bernd AU - Stahl B AD - Department of Immunology, Human Milk Research, Nutricia Research, Utrecht, Netherlands. FAU - Overbeek, Saskia A AU - Overbeek SA AD - Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, Utrecht, Netherlands. AD - Department of Immunology, Human Milk Research, Nutricia Research, Utrecht, Netherlands. FAU - Garssen, Johan AU - Garssen J AD - Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, Utrecht, Netherlands. AD - Department of Immunology, Human Milk Research, Nutricia Research, Utrecht, Netherlands. FAU - Folkerts, Gert AU - Folkerts G AD - Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, Utrecht, Netherlands. FAU - Van't Land, Belinda AU - Van't Land B AD - Department of Immunology, Human Milk Research, Nutricia Research, Utrecht, Netherlands. AD - Department of Paediatric Immunology, Wilhelmina Children's Hospital, University Medical Center Utrecht, Utrecht, Netherlands. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20180309 PL - Switzerland TA - Front Immunol JT - Frontiers in immunology JID - 101560960 RN - 0 (Antibodies) RN - 0 (Influenza Vaccines) RN - 0 (Oligosaccharides) RN - 0 (Trisaccharides) RN - 9006-59-1 (Ovalbumin) RN - XO2533XO8R (2'-fucosyllactose) SB - IM MH - Adaptive Immunity MH - Animals MH - Antibodies/blood MH - Disease Models, Animal MH - Female MH - Humans MH - Hypersensitivity, Delayed/*immunology MH - Immunity, Innate MH - Influenza A virus/*immunology MH - Influenza Vaccines/*immunology MH - Influenza, Human/*immunology MH - Mice MH - Mice, Inbred C57BL MH - Milk MH - Oligosaccharides/*immunology MH - Ovalbumin/*immunology MH - Trisaccharides/*immunology MH - Vaccination PMC - PMC5854647 OTO - NOTNLM OT - 2'fucosyllactose OT - B-cell activation OT - delayed-type hypersensitivity OT - diet OT - human milk oligosaccharides OT - immune response OT - vaccination EDAT- 2018/03/30 06:00 MHDA- 2018/03/30 06:01 PMCR- 2018/01/01 CRDT- 2018/03/30 06:00 PHST- 2018/01/18 00:00 [received] PHST- 2018/02/20 00:00 [accepted] PHST- 2018/03/30 06:00 [entrez] PHST- 2018/03/30 06:00 [pubmed] PHST- 2018/03/30 06:01 [medline] PHST- 2018/01/01 00:00 [pmc-release] AID - 10.3389/fimmu.2018.00452 [doi] PST - epublish SO - Front Immunol. 2018 Mar 9;9:452. doi: 10.3389/fimmu.2018.00452. eCollection 2018.