PMID- 29596368
OWN - NLM
STAT- MEDLINE
DCOM- 20180906
LR  - 20181207
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 23
IP  - 4
DP  - 2018 Mar 29
TI  - UHPLC-MS Metabolome Fingerprinting: The Isolation of Main Compounds and 
      Antioxidant Activity of the Andean Species Tetraglochin ameghinoi (Speg.) Speg.
LID - 10.3390/molecules23040793 [doi]
LID - 793
AB  - The seriated extracts of petroleum ether (PE-E), dichloromethane (DCM-E) and 
      methanol extracts (MeOH-E) from the aerial parts of the native South American 
      plant Tetraglochin ameghinoi (Rosaceae), were evaluated regarding their 
      antioxidant and antibacterial activities. The antioxidant properties were 
      evaluated by free radical scavenging methods (DPPH and TEAC), ferric-reducing 
      antioxidant power (FRAP) and lipoperoxidation in erythrocytes (LP), while the 
      antibacterial activity was performed against Gram-positive and Gram-negative 
      bacteria according to the Clinical and Laboratory Standards Institute (CLSI) 
      guidelines. The chemical and biological analyses of this plant are very important 
      since this bush is currently used in traditional medicine as a cholagogue and 
      digestive. The polar MeOH-E showed the highest antioxidant activities (17.70 
      microg/mL in the DPPH assay, 381.43 +/- 22.38 mM TE/g extract in the FRAP assay, 387.76 
      +/- 91.93 mg TE/g extract in the TEAC assay and 93.23 + 6.77% in the LP assay) and 
      it was selected for chromatographic isolation of its components. These components 
      were found to be four acetophenones, including the new phloracetophenone 
      glucoside: 4',6',-dihydroxy-2'-O-(6''-acetyl)-beta-d-glucopyranosylacetophenone or 
      IUPAC name: 
      (6-(2-acetyl-3,5-dihydroxyphenoxy)-3,4,5-trihydroxytetrahydro-2H-pyran-2-yl)methyl 
      acetate, whose structure was elucidated by NMR and MS methods. In addition, 
      twenty-six compounds, including five of these acetophenone derivatives, two 
      sugars, six flavonoids, eleven phenolic acids and two triterpenes, were 
      identified based on UHPLC-OT-MS and PDA analysis on the MeOH-E. The results 
      support the medicinal use of the plant.
FAU - Luna, Lorena
AU  - Luna L
AD  - Instituto de Biotecnologia-Instituto de Ciencias Basicas, Universidad Nacional de 
      San Juan, Av. Li bertador General San Martin 1109 (O), San Juan CP5400, 
      Argentina. lluna@unsj.edu.ar.
FAU - Simirgiotis, Mario J
AU  - Simirgiotis MJ
AUID- ORCID: 0000-0003-4552-3123
AD  - Instituto de Farmacia, Facultad de Ciencias, Universidad Austral de Chile, Campus 
      Isla Teja, Valdivia 5090000, Chile. mario.simirgiotis@gmail.com.
AD  - Center for Interdisciplinary Studies on the Nervous System (CISNe), Universidad 
      Austral de Chile, Valdivia 5090000, Chile. mario.simirgiotis@gmail.com.
FAU - Lima, Beatriz
AU  - Lima B
AD  - Instituto de Biotecnologia-Instituto de Ciencias Basicas, Universidad Nacional de 
      San Juan, Av. Li bertador General San Martin 1109 (O), San Juan CP5400, 
      Argentina. blima@unsj.edu.ar.
AD  - CONICET (Consejo Nacional de Ciencia y Tecnologia), CABA, Buenos Aires C1405DJR, 
      Argentina. blima@unsj.edu.ar.
FAU - Borquez, Jorge
AU  - Borquez J
AD  - Laboratorio de Productos Naturales Depto. de Quimica, Facultad de Ciencias, 
      Universidad de Antofagasta, Av. Coloso S-N, Antofagasta 1240000, Chile. 
      jorge.borquez@uantof.cl.
FAU - Feresin, Gabriela E
AU  - Feresin GE
AD  - Instituto de Biotecnologia-Instituto de Ciencias Basicas, Universidad Nacional de 
      San Juan, Av. Li bertador General San Martin 1109 (O), San Juan CP5400, 
      Argentina. gferesin@unsj.edu.ar.
AD  - CONICET (Consejo Nacional de Ciencia y Tecnologia), CABA, Buenos Aires C1405DJR, 
      Argentina. gferesin@unsj.edu.ar.
FAU - Tapia, Alejandro
AU  - Tapia A
AD  - Instituto de Biotecnologia-Instituto de Ciencias Basicas, Universidad Nacional de 
      San Juan, Av. Li bertador General San Martin 1109 (O), San Juan CP5400, 
      Argentina. atapia@unsj.edu.ar.
LA  - eng
PT  - Journal Article
DEP - 20180329
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Antioxidants)
SB  - IM
MH  - Antioxidants/chemistry/*metabolism
MH  - Metabolome/*physiology
MH  - *Metabolomics
MH  - Rosaceae/chemistry/*metabolism
PMC - PMC6017344
OTO - NOTNLM
OT  - UHPLC Orbitrap (Q-OT)
OT  - acetophenones
OT  - antioxidant
OT  - phenolic
OT  - triterpenes
COIS- The authors declare no conflict of interest.
EDAT- 2018/03/30 06:00
MHDA- 2018/09/07 06:00
PMCR- 2018/03/29
CRDT- 2018/03/30 06:00
PHST- 2018/02/08 00:00 [received]
PHST- 2018/03/22 00:00 [revised]
PHST- 2018/03/27 00:00 [accepted]
PHST- 2018/03/30 06:00 [entrez]
PHST- 2018/03/30 06:00 [pubmed]
PHST- 2018/09/07 06:00 [medline]
PHST- 2018/03/29 00:00 [pmc-release]
AID - molecules23040793 [pii]
AID - molecules-23-00793 [pii]
AID - 10.3390/molecules23040793 [doi]
PST - epublish
SO  - Molecules. 2018 Mar 29;23(4):793. doi: 10.3390/molecules23040793.