PMID- 2959679
OWN - NLM
STAT- MEDLINE
DCOM- 19871125
LR  - 20061115
IS  - 0021-972X (Print)
IS  - 0021-972X (Linking)
VI  - 65
IP  - 5
DP  - 1987 Nov
TI  - Dysfunction of suppressor T cells in thyroid glands from patients with Graves' 
      disease.
PG  - 922-8
AB  - To investigate the suppressor function of intrathyroidal (TG) T cells in Graves' 
      disease, the percentage of suppressor T cell subsets and the suppressor function 
      of TG and peripheral blood (PB) lymphocytes in Graves' disease were compared by 
      determining the in vitro production of immunoglobulin G (IgG) in reconstituted 
      mixtures of separated B, CD4+ (helper/suppressor-inducer T), and CD8+ 
      (suppressor/cytotoxic T) cells. TG lymphocytes were obtained by gradient 
      centrifugation of the supernatants of minced thyroid tissues. T Cells were 
      separated by E-rosette formation, and CD4+ and CD8+ cell-rich populations were 
      separated by a panning method using monoclonal anti-CD8 antibody. Mixtures of 5 X 
      10(4) B (PB1 or TG) cells, 2 X 10(4) CD4+ (PB or TG) cells, and 5 X 10(3) 
      macrophages (PB or TG) were cultured with various numbers of CD8+ (PB) or CD8+ 
      (TG) cells for 7 days with pokeweed mitogen, and IgG synthesis was determined by 
      solid phase RIA. T cell subpopulations were quantitated by a direct 
      immunofluorescence method using monoclonal anti-CD3, anti-CD4, and anti-CD8 
      antibodies. There was no difference in the percentages of CD8+ cells among total 
      T cells between thyroid glands and peripheral blood from Graves' disease patients 
      [mean PB, 39.8 +/- 9.8% (+/- SD); TG, 42.5 +/- 13.8%; n = 10]. IgG production by 
      mixtures of B and CD4+ cells isolated from peripheral blood was not different 
      from that by cells isolated from thyroid glands [mean PB, 1635 +/- 248 (+/- SE) 
      ng/mL; TG, 1081 +/- 128 ng/mL; n = 19; P = NS]. The nonspecific suppressor 
      activity of thyroid gland CD8+ cells was less than that of CD8+ (PB) cells 
      [percent suppression of IgG production by mixtures of B (PB) and CD4+ (PB) cells, 
      12.5% vs. 57.0% (P less than 0.01); by mixtures of B (TG) and CD4+ (TG) cells, 
      5.8% vs. 38.9% (P less than 0.01)]. The suppressor-inducer function of CD4+ (TG) 
      cells was also decreased compared with that of CD4+ (PB) cells. These results 
      suggest that the impairment of suppressor cell activity may lead to excessive 
      production of autoantibody in thyroid glands from patients with Graves' disease.
FAU - Ueki, Y
AU  - Ueki Y
AD  - First Department of Internal Medicine, Nagasaki University School of Medicine, 
      Japan.
FAU - Eguchi, K
AU  - Eguchi K
FAU - Fukuda, T
AU  - Fukuda T
FAU - Otsubo, T
AU  - Otsubo T
FAU - Kawabe, Y
AU  - Kawabe Y
FAU - Shimomura, C
AU  - Shimomura C
FAU - Matsunaga, M
AU  - Matsunaga M
FAU - Tezuka, H
AU  - Tezuka H
FAU - Ishikawa, N
AU  - Ishikawa N
FAU - Ito, K
AU  - Ito K
AU  - et al.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - J Clin Endocrinol Metab
JT  - The Journal of clinical endocrinology and metabolism
JID - 0375362
RN  - 0 (Immunoglobulin G)
RN  - 0 (Pokeweed Mitogens)
SB  - IM
MH  - Adult
MH  - B-Lymphocytes/metabolism/physiology
MH  - Blood Cells/metabolism/physiology
MH  - Female
MH  - Graves Disease/metabolism/pathology/*physiopathology
MH  - Humans
MH  - Immunoglobulin G/biosynthesis
MH  - Male
MH  - Pokeweed Mitogens/pharmacology
MH  - T-Lymphocytes/classification
MH  - T-Lymphocytes, Cytotoxic/metabolism/physiology
MH  - T-Lymphocytes, Helper-Inducer/metabolism/physiology
MH  - T-Lymphocytes, Regulatory/metabolism/*physiology
MH  - Thyroid Gland/metabolism/pathology/*physiopathology
EDAT- 1987/11/01 00:00
MHDA- 1987/11/01 00:01
CRDT- 1987/11/01 00:00
PHST- 1987/11/01 00:00 [pubmed]
PHST- 1987/11/01 00:01 [medline]
PHST- 1987/11/01 00:00 [entrez]
AID - 10.1210/jcem-65-5-922 [doi]
PST - ppublish
SO  - J Clin Endocrinol Metab. 1987 Nov;65(5):922-8. doi: 10.1210/jcem-65-5-922.