PMID- 29601942
OWN - NLM
STAT- MEDLINE
DCOM- 20190503
LR  - 20190503
IS  - 1879-0542 (Electronic)
IS  - 0165-2478 (Linking)
VI  - 198
DP  - 2018 Jun
TI  - Effects of Toll-like receptor 4 on beta2-glycoprotein I-induced splenic T cell 
      subsets differentiation.
PG  - 17-25
LID - S0165-2478(17)30408-X [pii]
LID - 10.1016/j.imlet.2018.03.010 [doi]
AB  - Our previous study demonstrated that beta 2-glycoprotein I (beta2GPI) stimulation 
      promotes bone marrow derived dendritic cells (BMDCs) maturation and T cell 
      proliferation in a Toll-like receptor 4 (TLR4) dependent manner. However, beta2GPI 
      induced T cell differentiation and the role of TLR4 in this process have rarely 
      been reported. In the present study, we focused on the differentiation of splenic 
      T cells in beta2GPI immunized Balb/c, C3H/HeN and C3H/HeJ mice. According to our 
      results, Th2 dominated differentiation was observed in beta2GPI immunized Balb/c and 
      C3H/HeN mice than in those treated with normal saline (NS), namely the 
      up-regulated levels of Th2 markers GATA3 and IL-4 (p < 0.05). Meanwhile, reduced 
      Th1 markers T-bet and IFN-gamma, and Treg marker Foxp3 were observed in beta2GPI 
      immunized mice (p < 0.05). C3H/HeJ mice have the same gene background with 
      C3H/HeN mice except a functional mutant in TLR4 gene. However, the described Th2 
      differentiation was not detected in these TLR4 deficient mice, indicating the 
      importance of TLR4 in immune response against beta2GPI. In addition, we found that 
      beta2GPI-induced Th2 differentiation could be strengthened by cytokines secreted by 
      dendritic cells (DCs) and DCs-T cells interaction. However, DCs-T cells contact 
      was indispensable during this process because of its unique role in suppressing 
      Th1 function. Furthermore, this Th2 biased differentiation pattern was more 
      noticeable in mice received 4 times beta2GPI immunization than those received 2 
      times, suggesting the amplifying effects of anti-beta2GPI Ab on beta2GPI induced Th2 
      response. These findings may partly explain the immune imbalance in APS patient 
      through the view angle of T cell differentiation and anti-beta2GPI antibody 
      production.
CI  - Copyright (c) 2018 European Federation of Immunological Societies. Published by 
      Elsevier B.V. All rights reserved.
FAU - He, Chao
AU  - He C
AD  - Department of Clinical Laboratory and Hematology, School of Medicine, Jiangsu 
      University, Zhenjiang, Jiangsu 212013, PR China.
FAU - Zhang, Guiting
AU  - Zhang G
AD  - Department of Clinical Laboratory and Hematology, School of Medicine, Jiangsu 
      University, Zhenjiang, Jiangsu 212013, PR China.
FAU - Zhou, Hong
AU  - Zhou H
AD  - Department of Clinical Laboratory and Hematology, School of Medicine, Jiangsu 
      University, Zhenjiang, Jiangsu 212013, PR China. Electronic address: 
      hongzhou@ujs.edu.cn.
FAU - Cheng, Si
AU  - Cheng S
AD  - Department of Clinical Laboratory and Hematology, School of Medicine, Jiangsu 
      University, Zhenjiang, Jiangsu 212013, PR China.
FAU - Farwa, Amel
AU  - Farwa A
AD  - Department of Clinical Laboratory and Hematology, School of Medicine, Jiangsu 
      University, Zhenjiang, Jiangsu 212013, PR China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180327
PL  - Netherlands
TA  - Immunol Lett
JT  - Immunology letters
JID - 7910006
RN  - 0 (Antibodies, Antiphospholipid)
RN  - 0 (Cytokines)
RN  - 0 (Tlr4 protein, mouse)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (beta 2-Glycoprotein I)
SB  - IM
MH  - Animals
MH  - Antibodies, Antiphospholipid/immunology
MH  - Antigen Presentation
MH  - Cell Differentiation/*immunology
MH  - Cytokines/metabolism
MH  - Dendritic Cells/immunology/metabolism
MH  - Immunization
MH  - Lymphocyte Activation
MH  - Male
MH  - Mice
MH  - Spleen/*immunology
MH  - T-Lymphocyte Subsets/*immunology
MH  - Th1 Cells/immunology
MH  - Th2 Cells/immunology
MH  - Toll-Like Receptor 4/deficiency/*immunology
MH  - beta 2-Glycoprotein I/*immunology
OTO - NOTNLM
OT  - APS
OT  - Anti-beta2-glycoprotein I antibody
OT  - Autoimmunity
OT  - Th cell
OT  - Toll-like receptor 4
EDAT- 2018/03/31 06:00
MHDA- 2019/05/06 06:00
CRDT- 2018/03/31 06:00
PHST- 2017/08/16 00:00 [received]
PHST- 2018/02/12 00:00 [revised]
PHST- 2018/03/23 00:00 [accepted]
PHST- 2018/03/31 06:00 [pubmed]
PHST- 2019/05/06 06:00 [medline]
PHST- 2018/03/31 06:00 [entrez]
AID - S0165-2478(17)30408-X [pii]
AID - 10.1016/j.imlet.2018.03.010 [doi]
PST - ppublish
SO  - Immunol Lett. 2018 Jun;198:17-25. doi: 10.1016/j.imlet.2018.03.010. Epub 2018 Mar 
      27.