PMID- 29602282
OWN - NLM
STAT- MEDLINE
DCOM- 20190225
LR  - 20190225
IS  - 1559-4106 (Electronic)
IS  - 1559-4106 (Linking)
VI  - 13
IP  - 3
DP  - 2018 Mar 30
TI  - Comparison between thaw-mounting and use of conductive tape for sample 
      preparation in ToF-SIMS imaging of lipids in Drosophila microRNA-14 model.
PG  - 03B414
LID - 10.1116/1.5019597 [doi]
AB  - Time-of-flight secondary ion mass spectrometry (ToF-SIMS) imaging elucidates 
      molecular distributions in tissue sections, providing useful information about 
      the metabolic pathways linked to diseases. However, delocalization of the 
      analytes and inadequate tissue adherence during sample preparation are among some 
      of the unfortunate phenomena associated with this technique due to their role in 
      the reduction of the quality, reliability, and spatial resolution of the ToF-SIMS 
      images. For these reasons, ToF-SIMS imaging requires a more rigorous sample 
      preparation method in order to preserve the natural state of the tissues. The 
      traditional thaw-mounting method is particularly vulnerable to altered 
      distributions of the analytes due to thermal effects, as well as to tissue 
      shrinkage. In the present study, the authors made comparisons of different tissue 
      mounting methods, including the thaw-mounting method. The authors used conductive 
      tape as the tissue-mounting material on the substrate because it does not require 
      heat from the finger for the tissue section to adhere to the substrate and can 
      reduce charge accumulation during data acquisition. With the conductive-tape 
      sampling method, they were able to acquire reproducible tissue sections and 
      high-quality images without redistribution of the molecules. Also, the authors 
      were successful in preserving the natural states and chemical distributions of 
      the different components of fat metabolites such as diacylglycerol and fatty 
      acids by using the tape-supported sampling in microRNA-14 (miR-14) deleted 
      Drosophila models. The method highlighted here shows an improvement in the 
      accuracy of mass spectrometric imaging of tissue samples.
FAU - Le, Minh Uyen Thi
AU  - Le MUT
AD  - Department of Nano Science, University of Science and Technology (UST), Daejeon 
      34113, South Korea and Center for Nano-Bio Measurement, Korea Research Institute 
      of Standards and Science (KRISS), Daejeon 34113, South Korea.
FAU - Son, Jin Gyeong
AU  - Son JG
AD  - Center for Nano-Bio Measurement, Korea Research Institute of Standards and 
      Science (KRISS), Daejeon 34113, South Korea.
FAU - Shon, Hyun Kyoung
AU  - Shon HK
AD  - Center for Nano-Bio Measurement, Korea Research Institute of Standards and 
      Science (KRISS), Daejeon 34113, South Korea.
FAU - Park, Jeong Hyang
AU  - Park JH
AD  - Department of Brain and Cognitive Sciences, Daegu Gyeongbuk Institute of Science 
      and Technology (DGIST), Daegu 42988, South Korea.
FAU - Lee, Sung Bae
AU  - Lee SB
AD  - Department of Brain and Cognitive Sciences, Daegu Gyeongbuk Institute of Science 
      and Technology (DGIST), Daegu 42988, South Korea.
FAU - Lee, Tae Geol
AU  - Lee TG
AD  - Department of Nano Science, University of Science and Technology (UST), Daejeon 
      34113, South Korea and Center for Nano-Bio Measurement, Korea Research Institute 
      of Standards and Science (KRISS), Daejeon 34113, South Korea.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180330
PL  - United States
TA  - Biointerphases
JT  - Biointerphases
JID - 101275679
RN  - 0 (Lipids)
RN  - 0 (MIRN14 microRNA, Drosophila)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Animals
MH  - Drosophila/*chemistry/genetics
MH  - Gene Deletion
MH  - Histocytological Preparation Techniques/*methods
MH  - Image Processing, Computer-Assisted/*methods
MH  - Lipids/*analysis
MH  - MicroRNAs/genetics
MH  - Reproducibility of Results
MH  - Spectrometry, Mass, Secondary Ion/*methods
EDAT- 2018/04/01 06:00
MHDA- 2019/02/26 06:00
CRDT- 2018/04/01 06:00
PHST- 2018/04/01 06:00 [entrez]
PHST- 2018/04/01 06:00 [pubmed]
PHST- 2019/02/26 06:00 [medline]
AID - 10.1116/1.5019597 [doi]
PST - epublish
SO  - Biointerphases. 2018 Mar 30;13(3):03B414. doi: 10.1116/1.5019597.