PMID- 2960385
OWN - NLM
STAT- MEDLINE
DCOM- 19880121
LR  - 20181113
IS  - 0006-3495 (Print)
IS  - 1542-0086 (Electronic)
IS  - 0006-3495 (Linking)
VI  - 52
IP  - 4
DP  - 1987 Oct
TI  - The effect of receptor density on the forward rate constant for binding of 
      ligands to cell surface receptors.
PG  - 657-62
AB  - For monovalent ligands interacting with cell surface receptors we have directly 
      observed the functional dependence of the forward rate constant on the number of 
      receptors per cell (N). The experimental system we studied consisted of 
      monovalent ligand, 2,4-dinitrophenyl (DNP)-aminocaproyl-L-tyrosine (DCT), binding 
      to bivalent, monoclonal anti-DNP immunoglobulin E (IgE) anchored to its high 
      affinity receptor on rat basophilic leukemia (RBL) cells. To measure the 
      fractional occupation of antibody combining sites by DNP we employed a recently 
      developed fluorescence technique (Erickson, J., Kane, B. Goldstein, D. Holowka, 
      and B. Baird, 1986, Mol. Immunol., 72:769-781). Our results are well fitted by 
      the equation (Berg and Purcell, 1977, Biophys. J., 20:193-219) konc = 4 pi DaN 
      kappa on/[4 pi Da + N kappa on] where konc is the forward rate constant for 
      binding to the cell, D is the diffusion constant of the ligand, a is the radius 
      of the cell, and kappa on is the intrinsic forward rate constant describing a 
      single IgE combining site-DNP interaction. If D is fixed at 10(-5) cm2/s, the 
      best fit of accumulated data predicts an average cell radius of approximately 4 
      microns and kappa on of approximately 1.8 x 10(-13) cm3/s [1.1 x 10(8)(M . s)-1]; 
      both in excellent agreement with RBL cell size and the single-site forward rate 
      constant for the binding of DCT to IgE in solution, respectively. We believe this 
      is the first report of experimental evidence that directly illustrates the effect 
      of surface density in determining the rates of binding for small molecules to 
      membrane receptors.
FAU - Erickson, J
AU  - Erickson J
AD  - Department of Chemistry, Cornell University, Ithaca, New York 14853.
FAU - Goldstein, B
AU  - Goldstein B
FAU - Holowka, D
AU  - Holowka D
FAU - Baird, B
AU  - Baird B
LA  - eng
GR  - AI18306/AI/NIAID NIH HHS/United States
GR  - GM35556/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biophys J
JT  - Biophysical journal
JID - 0370626
RN  - 0 (Fluoresceins)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Ligands)
RN  - 0 (Receptors, Drug)
RN  - 0 (Receptors, Fc)
RN  - 0 (Receptors, IgE)
RN  - 0 (Thiocyanates)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - I223NX31W9 (Fluorescein-5-isothiocyanate)
SB  - IM
MH  - Animals
MH  - Cell Membrane/immunology/*metabolism
MH  - Fluorescein-5-isothiocyanate
MH  - Fluoresceins
MH  - Fluorescent Dyes
MH  - Immunoglobulin E/*metabolism
MH  - Kinetics
MH  - Ligands
MH  - Protein Binding
MH  - Receptors, Drug/*metabolism
MH  - Receptors, Fc/*metabolism
MH  - Receptors, IgE
MH  - Spectrometry, Fluorescence
MH  - Thiocyanates
PMC - PMC1330059
EDAT- 1987/10/01 00:00
MHDA- 1987/10/01 00:01
PMCR- 1988/10/01
CRDT- 1987/10/01 00:00
PHST- 1987/10/01 00:00 [pubmed]
PHST- 1987/10/01 00:01 [medline]
PHST- 1987/10/01 00:00 [entrez]
PHST- 1988/10/01 00:00 [pmc-release]
AID - S0006-3495(87)83258-7 [pii]
AID - 10.1016/S0006-3495(87)83258-7 [doi]
PST - ppublish
SO  - Biophys J. 1987 Oct;52(4):657-62. doi: 10.1016/S0006-3495(87)83258-7.