PMID- 2960615
OWN - NLM
STAT- MEDLINE
DCOM- 19880112
LR  - 20151119
IS  - 0300-5038 (Print)
IS  - 0300-5038 (Linking)
IP  - 84
DP  - 1987
TI  - Activation of N-nitrosodialkylamines by near-ultraviolet irradiation: formation 
      of directly-acting mutagens and DNA-damaging products.
PG  - 364-6
AB  - On near-ultraviolet (UVA) irradiation in a phosphate buffer, N-nitrosomorpholine 
      (NMOR) and N-nitrosopyrrolidine (NPYR) were converted into directly-acting 
      mutagens. The activated NPYR was fractionated, and the active product was 
      isolated. The compound was shown to be identical to alpha-phosphonooxy NPYR on 
      the basis of several properties: retention times in high-performance liquid 
      chromatograms, mutagenic specificity and potency, ultraviolet spectrum, and 
      inactivation by phosphatase treatment. Photoactivation was inhibited by 
      superoxide dismutase, and therefore superoxide is implicated as playing a key 
      role in mutagen formation. N-Nitrosoproline (NPRO) and eighteen other 
      N-nitrosodialkylamines were irradiated with UVA in the presence of phi X174 RFI 
      DNA. The DNA underwent single-strand breaks to give RFII DNA, indicating that 
      N-nitrosodialkylamines in general have this property. DNA chain cleavage was 
      inhibited both by superoxide dismutase and hydroxyl-radical scavengers. These 
      results provide new information on the genotoxic mechanism of action of 
      N-nitrosodialkylamines.
FAU - Shimada, H
AU  - Shimada H
AD  - Faculty of Pharmaceutical Sciences, Okayama University, Japan.
FAU - Yakushi, K
AU  - Yakushi K
FAU - Ikarashi, A
AU  - Ikarashi A
FAU - Mochizuki, M
AU  - Mochizuki M
FAU - Suzuki, E
AU  - Suzuki E
FAU - Okada, M
AU  - Okada M
FAU - Yokoyama, S
AU  - Yokoyama S
FAU - Miyazawa, T
AU  - Miyazawa T
FAU - Hayatsu, H
AU  - Hayatsu H
LA  - eng
PT  - Journal Article
PL  - France
TA  - IARC Sci Publ
JT  - IARC scientific publications
JID - 8009542
RN  - 0 (DNA, Single-Stranded)
RN  - 0 (Nitrosamines)
RN  - 3L25FO7FN7 (N-nitrosomorpholine)
RN  - F8MI03SGY0 (nitrosoproline)
RN  - SZ4J5WK201 (N-Nitrosopyrrolidine)
SB  - IM
MH  - Bacteriophage phi X 174
MH  - DNA Damage/*drug effects
MH  - DNA, Single-Stranded/drug effects/radiation effects
MH  - Mutagenicity Tests
MH  - N-Nitrosopyrrolidine/radiation effects
MH  - Nitrosamines/*radiation effects/toxicity
MH  - Ultraviolet Rays
EDAT- 1987/01/01 00:00
MHDA- 1987/01/01 00:01
CRDT- 1987/01/01 00:00
PHST- 1987/01/01 00:00 [pubmed]
PHST- 1987/01/01 00:01 [medline]
PHST- 1987/01/01 00:00 [entrez]
PST - ppublish
SO  - IARC Sci Publ. 1987;(84):364-6.