PMID- 29607364
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 2347-3584 (Print)
IS  - 2347-3517 (Electronic)
IS  - 2347-3584 (Linking)
VI  - 29
IP  - 1
DP  - 2018 Mar
TI  - Detection of classical swine fever virus E2 gene in cattle serum samples from 
      cattle herds of Meghalaya.
PG  - 89-95
LID - 10.1007/s13337-018-0433-9 [doi]
AB  - The present study focused on the detection and genetic characterisation of 5' 
      untranslated region (5'UTR) and E2 gene of classical swine fever virus (CSFV, 
      family Flaviviridae, genus Pestivirus) from bovine population of the northeastern 
      region of India. A total of 134 cattle serum samples were collected from 
      organised cattle farms and were screened for CSFV antigen with a commercial 
      antigen capture enzyme linked immunosorbent assay (Ag-ELISA) and reverse 
      transcription-polymerase chain reaction (RT-PCR). A total of 10 samples were 
      positive for CSFV antigen by ELISA, while all of them were positive in PCR for 
      5'UTR region. Full length E2 region of CSFV were successfully amplified from two 
      positive samples and used for subsequent phylogenetic analysis and determination 
      of protein 3D structure which showed similarity with reported CSFV isolate from 
      Assam of sub-genogroup 2.1, with minor variations in protein structure.
FAU - Chakraborty, A K
AU  - Chakraborty AK
AD  - Division of Animal Health, ICAR RC for NEH Region, Umiam, Meghalaya India.
AD  - 2Department of Microbiology, Assam University, Silchar, Assam India. ISNI: 0000 
      0004 1767 4538. GRID: grid.411460.6
FAU - Karam, A
AU  - Karam A
AD  - Division of Animal Health, ICAR RC for NEH Region, Umiam, Meghalaya India.
FAU - Mukherjee, P
AU  - Mukherjee P
AD  - Division of Animal Health, ICAR RC for NEH Region, Umiam, Meghalaya India.
AD  - 2Department of Microbiology, Assam University, Silchar, Assam India. ISNI: 0000 
      0004 1767 4538. GRID: grid.411460.6
FAU - Barkalita, L
AU  - Barkalita L
AD  - Department of Biotechnology, C.V.Sc, AAU, Khanapara, Assam India.
FAU - Borah, P
AU  - Borah P
AD  - Department of Biotechnology, C.V.Sc, AAU, Khanapara, Assam India.
FAU - Das, S
AU  - Das S
AD  - Division of Animal Health, ICAR RC for NEH Region, Umiam, Meghalaya India.
FAU - Sanjukta, R
AU  - Sanjukta R
AD  - Division of Animal Health, ICAR RC for NEH Region, Umiam, Meghalaya India.
FAU - Puro, K
AU  - Puro K
AD  - Division of Animal Health, ICAR RC for NEH Region, Umiam, Meghalaya India.
FAU - Ghatak, S
AU  - Ghatak S
AD  - Division of Animal Health, ICAR RC for NEH Region, Umiam, Meghalaya India.
FAU - Shakuntala, I
AU  - Shakuntala I
AD  - Division of Animal Health, ICAR RC for NEH Region, Umiam, Meghalaya India.
FAU - Sharma, I
AU  - Sharma I
AD  - 2Department of Microbiology, Assam University, Silchar, Assam India. ISNI: 0000 
      0004 1767 4538. GRID: grid.411460.6
FAU - Laha, R G
AU  - Laha RG
AD  - Division of Animal Health, ICAR RC for NEH Region, Umiam, Meghalaya India.
FAU - Sen, A
AU  - Sen A
AD  - Division of Animal Health, ICAR RC for NEH Region, Umiam, Meghalaya India.
LA  - eng
PT  - Journal Article
DEP - 20180217
PL  - India
TA  - Virusdisease
JT  - Virusdisease
JID - 101624144
PMC - PMC5877855
OTO - NOTNLM
OT  - 5'UTR
OT  - CSFV
OT  - E2
OT  - I-TASSER
OT  - RT-PCR
EDAT- 2018/04/03 06:00
MHDA- 2018/04/03 06:01
PMCR- 2019/03/01
CRDT- 2018/04/03 06:00
PHST- 2017/07/19 00:00 [received]
PHST- 2018/01/20 00:00 [accepted]
PHST- 2018/04/03 06:00 [entrez]
PHST- 2018/04/03 06:00 [pubmed]
PHST- 2018/04/03 06:01 [medline]
PHST- 2019/03/01 00:00 [pmc-release]
AID - 433 [pii]
AID - 10.1007/s13337-018-0433-9 [doi]
PST - ppublish
SO  - Virusdisease. 2018 Mar;29(1):89-95. doi: 10.1007/s13337-018-0433-9. Epub 2018 Feb 
      17.