PMID- 29611586
OWN - NLM
STAT- MEDLINE
DCOM- 20180917
LR  - 20180917
IS  - 2042-650X (Electronic)
IS  - 2042-6496 (Linking)
VI  - 9
IP  - 4
DP  - 2018 Apr 25
TI  - Ultrasonicated Lespedeza cuneata extract prevents TNF-alpha-induced early 
      atherosclerosis in vitro and in vivo.
PG  - 2090-2101
LID - 10.1039/c7fo01666b [doi]
AB  - This study evaluated the use of ultrasonication to extract Lespedeza cuneata as a 
      potential nutraceutical for preventing vascular inflammation. Ultrasonicated L. 
      cuneata extract (ULCE) was prepared using 20% ethanol and 2 h of ultrasonication 
      at room temperature, and its effects were investigated using relevant in vitro 
      and in vivo models. ULCE suppressed tumor necrosis factor-alpha (TNF-alpha)-induced 
      adhesion capacity, vascular cell adhesion protein 1 (VCAM-1) expression, and 
      nuclear factor kappa-B (NF-kappaB) activity in human umbilical vein endothelial cells 
      (HUVECs). ULCE also suppressed TNF-alpha-induced NF-kappaB signaling pathways and p65 
      translocation from the cytosol to the nucleus, as well as the mRNA expression of 
      IL-1beta, IL-6, and TNF-alpha in HUVECs. Oral administration of ULCE suppressed 
      TNF-alpha-induced monocyte infiltration into the intima and VCAM-1 expression, as 
      well as the IL-1beta, IL-6, TNF-alpha, and monocyte chemoattractant protein-1 (MCP-1) 
      mRNA expression in the main artery in mice. Among the compounds identified in the 
      hydrolyzed ULCE, quercetin exhibited the strongest inhibitory effect against 
      TNF-alpha-induced cell adhesion capacity. These results demonstrate that ULCE 
      contains potent preventive factors against early atherosclerosis, which act by 
      suppressing the NF-kappaB and VCAM-1 signaling axis.
FAU - Ha, Su Jeong
AU  - Ha SJ
AD  - Division of Functional Food Research, Korea Food Research Institute, 245 
      Nongsaengmyeong-ro Wanju-gun, Jeollabuk-do 55365, Republic of Korea.
FAU - Lee, Jangho
AU  - Lee J
FAU - Song, Kyung-Mo
AU  - Song KM
FAU - Kim, Young Ho
AU  - Kim YH
FAU - Lee, Nam Hyouck
AU  - Lee NH
FAU - Kim, Young-Eon
AU  - Kim YE
FAU - Jung, Sung Keun
AU  - Jung SK
LA  - eng
PT  - Journal Article
PL  - England
TA  - Food Funct
JT  - Food & function
JID - 101549033
RN  - 0 (Anti-Inflammatory Agents, Non-Steroidal)
RN  - 0 (Plant Extracts)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Anti-Inflammatory Agents, Non-Steroidal/administration & 
      dosage/pharmacology/*therapeutic use
MH  - Atherosclerosis/chemically induced/*prevention & control
MH  - Disease Models, Animal
MH  - Endothelium, Vascular/drug effects
MH  - Human Umbilical Vein Endothelial Cells/drug effects
MH  - Humans
MH  - Lespedeza/*chemistry
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Monocytes/drug effects
MH  - Phytotherapy
MH  - Plant Extracts/administration & dosage/pharmacology/*therapeutic use
MH  - Tumor Necrosis Factor-alpha
EDAT- 2018/04/04 06:00
MHDA- 2018/09/18 06:00
CRDT- 2018/04/04 06:00
PHST- 2018/04/04 06:00 [pubmed]
PHST- 2018/09/18 06:00 [medline]
PHST- 2018/04/04 06:00 [entrez]
AID - 10.1039/c7fo01666b [doi]
PST - ppublish
SO  - Food Funct. 2018 Apr 25;9(4):2090-2101. doi: 10.1039/c7fo01666b.