PMID- 29614801 OWN - NLM STAT- MEDLINE DCOM- 20180918 LR - 20240314 IS - 1999-4915 (Electronic) IS - 1999-4915 (Linking) VI - 10 IP - 4 DP - 2018 Apr 2 TI - Passion Fruit Chlorotic Mottle Virus: Molecular Characterization of a New Divergent Geminivirus in Brazil. LID - 10.3390/v10040169 [doi] LID - 169 AB - Brazil is one of the major passion fruit producers worldwide. Viral diseases are among the most important constraints for passion fruit production. Here we identify and characterize a new passion fruit infecting-virus belonging to the family Geminiviridae: passion fruit chlorotic mottle virus (PCMoV). PCMoV is a divergent geminivirus unlike previously characterized passion fruit-infecting geminiviruses that belonged to the genus Begomovirus. Among the presently known geminiviruses, it is most closely related to, and shares ~62% genome-wide identity with citrus chlorotic dwarf associated virus (CCDaV) and camelia chlorotic dwarf associated virus (CaCDaV). The 3743 nt PCMoV genome encodes a capsid protein (CP) and replication-associated protein (Rep) that respectively share 56 and 60% amino acid identity with those encoded by CaCDaV. The CPs of PCMoV, CCDaV, and CaCDaV cluster with those of begomovirus whereas their Reps with those of becurtoviruses. Hence, these viruses likely represent a lineage of recombinant begomo-like and becurto-like ancestral viruses. Furthermore, PCMoV, CCDaV, and CaCDaV genomes are ~12-30% larger than monopartite geminiviruses and this is primarily due to the encoded movement protein (MP; 891-921 nt) and this MP is most closely related to that encoded by the DNA-B component of bipartite begomoviruses. Hence, PCMoV, CCDaV, and CaCDaV lineage of viruses may represent molecules in an intermediary step in the evolution of bipartite begomoviruses (~5.3 kb) from monopartite geminiviruses (~2.7-3 kb). An infectious clone of PCMoV systemically infected Nicotiana benthamina, Arabidopsis thaliana, and Passiflora edulis. FAU - Fontenele, Rafaela S AU - Fontenele RS AD - Embrapa Recursos Geneticos e Biotecnologia, Brasilia, DF 70770-017, Brazil. rfontene@asu.edu. AD - The Biodesign Center for Fundamental and Applied Microbiomics, Center for Evolution and Medicine, School of Life Sciences, Arizona State University, Tempe, AZ 85287, USA. rfontene@asu.edu. FAU - Abreu, Rayane A AU - Abreu RA AD - Embrapa Recursos Geneticos e Biotecnologia, Brasilia, DF 70770-017, Brazil. rayannyalexandre@gmail.com. AD - Centro de Educacao e Saude, Universidade Federal de Campina Grande, Cuite, PB 58175-000, Brazil. rayannyalexandre@gmail.com. FAU - Lamas, Natalia S AU - Lamas NS AD - Embrapa Recursos Geneticos e Biotecnologia, Brasilia, DF 70770-017, Brazil. nslamas@gmail.com. FAU - Alves-Freitas, Dione M T AU - Alves-Freitas DMT AD - Embrapa Recursos Geneticos e Biotecnologia, Brasilia, DF 70770-017, Brazil. dionebio@gmail.com. FAU - Vidal, Andreza H AU - Vidal AH AD - Embrapa Recursos Geneticos e Biotecnologia, Brasilia, DF 70770-017, Brazil. andrezactg@hotmail.com. AD - Centro de Educacao e Saude, Universidade Federal de Campina Grande, Cuite, PB 58175-000, Brazil. andrezactg@hotmail.com. FAU - Poppiel, Raul R AU - Poppiel RR AUID- ORCID: 0000-0002-1628-4154 AD - Embrapa Recursos Geneticos e Biotecnologia, Brasilia, DF 70770-017, Brazil. raulpoppiel@gmail.com. FAU - Melo, Fernando L AU - Melo FL AD - Departmento de Biologia Celular, Campus Universitario Darcy Ribeiro, Universidade de Brasilia, Brasilia, DF 70910-900, Brazil. flucasmelo@gmail.com. FAU - Lacorte, Cristiano AU - Lacorte C AD - Embrapa Recursos Geneticos e Biotecnologia, Brasilia, DF 70770-017, Brazil. cristiano.lacorte@embrapa.br. FAU - Martin, Darren P AU - Martin DP AD - Computational Biology Group, Institute of Infectious Disease and Molecular Medicine, Faculty of Health Sciences, University of Cape Town, Observatory 7925, South Africa. darrenpatrickmartin@gmail.com. FAU - Campos, Magnolia A AU - Campos MA AD - Centro de Educacao e Saude, Universidade Federal de Campina Grande, Cuite, PB 58175-000, Brazil. magnoliacp@gmail.com. FAU - Varsani, Arvind AU - Varsani A AUID- ORCID: 0000-0003-4111-2415 AD - The Biodesign Center for Fundamental and Applied Microbiomics, Center for Evolution and Medicine, School of Life Sciences, Arizona State University, Tempe, AZ 85287, USA. arvind.varsani@asu.edu. AD - Structural Biology Research Unit, Department of Integrative Biomedical Sciences, University of Cape Town, Observatory, Cape Town 7701, South Africa. arvind.varsani@asu.edu. FAU - Ribeiro, Simone G AU - Ribeiro SG AD - Embrapa Recursos Geneticos e Biotecnologia, Brasilia, DF 70770-017, Brazil. simone.ribeiro@embrapa.br. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20180402 PL - Switzerland TA - Viruses JT - Viruses JID - 101509722 SB - IM MH - Begomovirus/*classification/*genetics MH - Brazil MH - Computational Biology/methods MH - Geminiviridae/classification/genetics MH - Genome, Viral MH - High-Throughput Nucleotide Sequencing MH - Open Reading Frames MH - Passiflora/*virology MH - Phylogeny MH - Plant Diseases/virology MH - Sequence Analysis, DNA PMC - PMC5923463 OTO - NOTNLM OT - Passiflora sp. OT - geminivirus OT - passion fruit COIS- The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, and in the decision to publish the results. EDAT- 2018/04/05 06:00 MHDA- 2018/09/19 06:00 PMCR- 2018/04/01 CRDT- 2018/04/05 06:00 PHST- 2018/03/01 00:00 [received] PHST- 2018/03/27 00:00 [revised] PHST- 2018/03/30 00:00 [accepted] PHST- 2018/04/05 06:00 [entrez] PHST- 2018/04/05 06:00 [pubmed] PHST- 2018/09/19 06:00 [medline] PHST- 2018/04/01 00:00 [pmc-release] AID - v10040169 [pii] AID - viruses-10-00169 [pii] AID - 10.3390/v10040169 [doi] PST - epublish SO - Viruses. 2018 Apr 2;10(4):169. doi: 10.3390/v10040169.