PMID- 29615782
OWN - NLM
STAT- MEDLINE
DCOM- 20191003
LR  - 20191007
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 8
IP  - 1
DP  - 2018 Apr 3
TI  - Preventive Effect of Low Intensity Pulsed Ultrasound against Experimental 
      Cerebral Ischemia/Reperfusion Injury via Apoptosis Reduction and Brain-derived 
      Neurotrophic Factor Induction.
PG  - 5568
LID - 10.1038/s41598-018-23929-8 [doi]
LID - 5568
AB  - Stroke is known as the top 10 causes of death worldwide. Development of 
      effectively neuroprotective or preventive strategies for ischemia stroke is 
      imperative. For the purpose of stroke prevention, we tested the neuroprotective 
      effects of low-intensity pulsed ultrasound (LIPUS) on ischemic stroke. Adult 
      C57BL/6 mice were used to daily treatment with LIPUS for 5 days on left 
      hemisphere before middle cerebral artery occlusion (MCAO)-induced cerebral 
      ischemia/reperfusion injury. Western blotting and immunohistochemistry were 
      performed to assess the protein expressions of signaling molecules. Pretreatment 
      with LIPUS significantly ameliorated the brain ischemic damage, including the 
      reduction of neurological deficit score, infarct area, histopathological score, 
      and showed a better performance in neurological and behavior functions. LIPUS 
      pretreatment could also significantly decrease the neuronal cell apoptosis and 
      upregulation of apoptosis-related signaling molecules and downregulation of 
      brain-derived neurotrophic factor (BDNF) in brain tissues of MCAO-treated mice. 
      Furthermore, LIPUS significantly prevented the decreased cell viability, the 
      increased caspase-3 cleavage, and the decreased BDNF expression in 
      ischemia/reperfusion-treated microglial cells. These results demonstrate that 
      LIPUS effectively prevented the cerebral ischemia/reperfusion injury through 
      apoptosis reduction and BDNF induction in a MCAO mouse model. The neuroprotective 
      potential of LIPUS may provide a novel preventive strategy for ischemic stroke in 
      high-risk patients.
FAU - Chen, Chang-Mu
AU  - Chen CM
AD  - Division of Neurosurgery, Department of Surgery, National Taiwan University 
      Hospital and National Taiwan University, College of Medicine, Taipei, Taiwan. 
      cmchen10@ms27.hinet.net.
FAU - Wu, Cheng-Tien
AU  - Wu CT
AD  - Institute of Toxicology, College of Medicine, National Taiwan University, Taipei, 
      Taiwan.
FAU - Yang, Ting-Hua
AU  - Yang TH
AD  - Department of Otolaryngology, National Taiwan University Hospital, Taipei, 
      Taiwan.
FAU - Liu, Shing-Hwa
AU  - Liu SH
AD  - Institute of Toxicology, College of Medicine, National Taiwan University, Taipei, 
      Taiwan. shinghwaliu@ntu.eud.tw.
AD  - Department of Pediatrics, College of Medicine and Hospital, National Taiwan 
      University, Taipei, Taiwan. shinghwaliu@ntu.eud.tw.
AD  - Department of Medical Research, China Medical University Hospital, China Medical 
      University, Taichung, Taiwan. shinghwaliu@ntu.eud.tw.
FAU - Yang, Feng-Yi
AU  - Yang FY
AD  - Department of Biomedical Imaging and Radiological Sciences, National Yang-Ming 
      University, Taipei, Taiwan. fyyang@ym.edu.tw.
AD  - Biophotonics and Molecular Imaging Research Center, National Yang-Ming 
      University, Taipei, Taiwan. fyyang@ym.edu.tw.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180403
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Brain-Derived Neurotrophic Factor)
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - Brain Ischemia/*complications
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microglia/pathology
MH  - Reperfusion Injury/complications/*pathology/*prevention & control
MH  - Signal Transduction
MH  - *Ultrasonic Waves
PMC - PMC5882812
COIS- The authors declare no competing interests.
EDAT- 2018/04/05 06:00
MHDA- 2019/10/08 06:00
PMCR- 2018/04/03
CRDT- 2018/04/05 06:00
PHST- 2018/01/19 00:00 [received]
PHST- 2018/03/20 00:00 [accepted]
PHST- 2018/04/05 06:00 [entrez]
PHST- 2018/04/05 06:00 [pubmed]
PHST- 2019/10/08 06:00 [medline]
PHST- 2018/04/03 00:00 [pmc-release]
AID - 10.1038/s41598-018-23929-8 [pii]
AID - 23929 [pii]
AID - 10.1038/s41598-018-23929-8 [doi]
PST - epublish
SO  - Sci Rep. 2018 Apr 3;8(1):5568. doi: 10.1038/s41598-018-23929-8.