PMID- 29619224
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220316
IS  - 2051-4190 (Print)
IS  - 2051-4190 (Electronic)
IS  - 2051-4190 (Linking)
VI  - 28
DP  - 2018
TI  - Sperm aneuploidy and DNA fragmentation in unexplained recurrent pregnancy loss: a 
      multicenter case-control study.
PG  - 4
LID - 10.1186/s12610-018-0070-6 [doi]
LID - 4
AB  - BACKGROUND: Recurrent pregnancy loss (RPL) is defined as the loss of at least 
      three pregnancies in the first trimester. Although the most common cause is 
      embryo aneuploidy, and despite female checkup and couple karyotyping, in about 
      50% of cases RPL remain unexplained. Male implication has little been 
      investigated and results are discordant. In this context, we conducted a 
      multi-center prospective case-control study to investigate male gamete 
      implication in unexplained RPL. METHODS: A total of 33 cases and 27 controls were 
      included from three university hospitals. We investigated environmental and 
      family factors with a detailed questionnaire and andrological examination, sperm 
      characteristics, sperm DNA/chromatin status using the sperm chromatin structure 
      assay (SCSA) and terminal deoxynucleotidyl transferase dUTP nick end labeling 
      (TUNEL) and sperm aneuploidy using fluorescence in situ hybridization (FISH). The 
      Mann-Whitney test and the Wilcoxon or Fisher exact tests were used. A 
      non-parametric Spearman correlation was performed in order to analyze the 
      relationship between various sperm parameters and FISH and sperm DNA 
      fragmentation results. RESULTS: We found significant differences between cases 
      and controls in time to conceive, body mass index (BMI), family history of 
      infertility and living environment. In cases, total sperm motility and the 
      percentage of morphologically normal spermatozoa were significantly decreased. No 
      difference was found between cases and controls in sperm DNA fragmentation or 
      chromatin integrity. In cases, spermatozoa with aneuploidy, hyperhaploidy and 
      chromosome 18 disomy were significantly increased. CONCLUSIONS: This prospective 
      case-control study is one of the largest to examine environmental factors, sperm 
      characteristics, sperm DNA fragmentation and chromatin, and chromosome anomalies 
      in spermatozoa in relation to unexplained recurrent pregnancy loss. The 
      originality of our study lies in the comprehensive andrological examination and 
      search for risk factors and fertility history. Further studies are needed to 
      confirm the links between unexplained RPL and a male family history of 
      infertility or miscarriages. The increased sperm aneuploidy observed in 
      unexplained RPL supports a male etiology. These data pave the way for further 
      studies to demonstrate the value of preimplantation genetic screening in men with 
      increased sperm aneuploidy whose partners experience unexplained RPL.
FAU - Esquerre-Lamare, Camille
AU  - Esquerre-Lamare C
AD  - 1Research Group on Human Fertility EA 3694, University Paul Sabatier Toulouse 
      III, 330 av Grande Bretagne, 31059 Toulouse, France. ISNI: 0000 0001 0723 035X. 
      GRID: grid.15781.3a
AD  - 2CECOS Groupe d'Activite de Medecine de la Reproduction, CHU Toulouse, Toulouse, 
      France. ISNI: 0000 0001 1457 2980. GRID: grid.411175.7
FAU - Walschaerts, Marie
AU  - Walschaerts M
AD  - 1Research Group on Human Fertility EA 3694, University Paul Sabatier Toulouse 
      III, 330 av Grande Bretagne, 31059 Toulouse, France. ISNI: 0000 0001 0723 035X. 
      GRID: grid.15781.3a
AD  - 2CECOS Groupe d'Activite de Medecine de la Reproduction, CHU Toulouse, Toulouse, 
      France. ISNI: 0000 0001 1457 2980. GRID: grid.411175.7
FAU - Chansel Debordeaux, Lucie
AU  - Chansel Debordeaux L
AD  - 3CECOS Service Biologie de la Reproduction, CHU Bordeaux, Bordeaux, France. ISNI: 
      0000 0004 0593 7118. GRID: grid.42399.35
FAU - Moreau, Jessika
AU  - Moreau J
AD  - 1Research Group on Human Fertility EA 3694, University Paul Sabatier Toulouse 
      III, 330 av Grande Bretagne, 31059 Toulouse, France. ISNI: 0000 0001 0723 035X. 
      GRID: grid.15781.3a
AD  - 2CECOS Groupe d'Activite de Medecine de la Reproduction, CHU Toulouse, Toulouse, 
      France. ISNI: 0000 0001 1457 2980. GRID: grid.411175.7
FAU - Bretelle, Florence
AU  - Bretelle F
AD  - 4Service de Gynecologie-Obstetrique, AP-HM Hopital Nord Marseille, Marseille, 
      France. ISNI: 0000 0001 0407 1584. GRID: grid.414336.7
FAU - Isus, Francois
AU  - Isus F
AD  - 1Research Group on Human Fertility EA 3694, University Paul Sabatier Toulouse 
      III, 330 av Grande Bretagne, 31059 Toulouse, France. ISNI: 0000 0001 0723 035X. 
      GRID: grid.15781.3a
AD  - 5Andrologie, Groupe d'Activite de Medecine de la Reproduction, CHU Toulouse, 
      Toulouse, France. ISNI: 0000 0001 1457 2980. GRID: grid.411175.7
FAU - Karsenty, Gilles
AU  - Karsenty G
AD  - 6Service d'Urologie et Transplantation Renale, AP-HM La Conception, Marseille, 
      France. ISNI: 0000 0001 0407 1584. GRID: grid.414336.7
FAU - Monteil, Laetitia
AU  - Monteil L
AD  - 7Service de Genetique Medicale, CHU Toulouse, Toulouse, France. ISNI: 0000 0001 
      1457 2980. GRID: grid.411175.7
FAU - Perrin, Jeanne
AU  - Perrin J
AD  - 8CECOS Laboratoire de Biologie de la Reproduction, AP-HM La Conception, 
      Marseille, France. ISNI: 0000 0001 0407 1584. GRID: grid.414336.7
AD  - 9Mediterranean Institute for Biodiversity and Ecology (IMBE), Aix-Marseille 
      Universite, CNRS, IRD, Avignon University, Marseille, France. ISNI: 0000 0001 
      2190 2394. GRID: grid.7310.5
FAU - Papaxanthos-Roche, Aline
AU  - Papaxanthos-Roche A
AD  - 3CECOS Service Biologie de la Reproduction, CHU Bordeaux, Bordeaux, France. ISNI: 
      0000 0004 0593 7118. GRID: grid.42399.35
FAU - Bujan, Louis
AU  - Bujan L
AD  - 1Research Group on Human Fertility EA 3694, University Paul Sabatier Toulouse 
      III, 330 av Grande Bretagne, 31059 Toulouse, France. ISNI: 0000 0001 0723 035X. 
      GRID: grid.15781.3a
AD  - 2CECOS Groupe d'Activite de Medecine de la Reproduction, CHU Toulouse, Toulouse, 
      France. ISNI: 0000 0001 1457 2980. GRID: grid.411175.7
LA  - eng
PT  - Journal Article
DEP - 20180402
PL  - England
TA  - Basic Clin Androl
JT  - Basic and clinical andrology
JID - 101640161
PMC - PMC5879915
OTO - NOTNLM
OT  - Fluorescence in situ hybridization
OT  - Sperm DNA fragmentation
OT  - Sperm aneuploidy
OT  - Unexplained recurrent pregnancy loss
COIS- Done in the manuscript (Methods section).Not applicable.The authors declare that 
      they have no competing interests.Springer Nature remains neutral with regard to 
      jurisdictional claims in published maps and institutional affiliations.
EDAT- 2018/04/06 06:00
MHDA- 2018/04/06 06:01
PMCR- 2018/04/02
CRDT- 2018/04/06 06:00
PHST- 2018/01/18 00:00 [received]
PHST- 2018/03/08 00:00 [accepted]
PHST- 2018/04/06 06:00 [entrez]
PHST- 2018/04/06 06:00 [pubmed]
PHST- 2018/04/06 06:01 [medline]
PHST- 2018/04/02 00:00 [pmc-release]
AID - 70 [pii]
AID - 10.1186/s12610-018-0070-6 [doi]
PST - epublish
SO  - Basic Clin Androl. 2018 Apr 2;28:4. doi: 10.1186/s12610-018-0070-6. eCollection 
      2018.