PMID- 29619372
OWN - NLM
STAT- MEDLINE
DCOM- 20180917
LR  - 20181114
IS  - 2314-6141 (Electronic)
IS  - 2314-6133 (Print)
VI  - 2018
DP  - 2018
TI  - Irradiated Human Umbilical Vein Endothelial Cells Undergo Endothelial-Mesenchymal 
      Transition via the Snail/miR-199a-5p Axis to Promote the Differentiation of 
      Fibroblasts into Myofibroblasts.
PG  - 4135806
LID - 10.1155/2018/4135806 [doi]
LID - 4135806
AB  - Radiation induced pulmonary fibrosis (RIPF) is one of the major side effects of 
      radiotherapy for lung cancer. Previous studies have shown that endothelial cells 
      and activated myofibroblasts play a key role in RIPF. However, the interaction 
      between irradiated endothelial cells and activation of myofibroblasts has not 
      been reported. The aim of the present study was to examine whether irradiated 
      endothelial cells would affect the differentiation of fibroblasts into 
      myofibroblasts in the process of RIPF. In the current study, we used a coculture 
      system that allowed direct contact between human fetal lung fibroblasts (MRC-5) 
      and irradiated human umbilical vein endothelial cells (HUVECs). After 24 or 48 h, 
      cells were sorted by flow cytometry. Radiation induced endothelial-mesenchymal 
      transition (EndMT) by significantly increasing the expression of Snail and 
      vimentin and reducing the expression of CD31 in HUVECs. In addition, irradiation 
      of HUVECs induced the expression of collagen type I and alpha-smooth muscle actin 
      (alpha-SMA) in MRC-5 cells. Further investigation indicated that irradiation of 
      HUVECs induced the differentiation of fibroblasts into myofibroblasts through the 
      Snail/miR-199a-5p axis. We conclude that irradiated endothelial cells undergo 
      EndMT to promote differentiation of fibroblasts into myofibroblasts via the 
      Snail/miR-199a-5p axis.
FAU - Yi, Minxiao
AU  - Yi M
AUID- ORCID: 0000-0001-7957-2244
AD  - Department of Oncology, Tongji Hospital, Huazhong University of Science and 
      Technology, Wuhan, Hubei Province, China.
FAU - Liu, Bo
AU  - Liu B
AD  - Department of Oncology, Tongji Hospital, Huazhong University of Science and 
      Technology, Wuhan, Hubei Province, China.
FAU - Tang, Yang
AU  - Tang Y
AD  - Department of Oncology, Tongji Hospital, Huazhong University of Science and 
      Technology, Wuhan, Hubei Province, China.
FAU - Li, Fang
AU  - Li F
AD  - Department of Oncology, Tongji Hospital, Huazhong University of Science and 
      Technology, Wuhan, Hubei Province, China.
FAU - Qin, Wan
AU  - Qin W
AD  - Department of Oncology, Tongji Hospital, Huazhong University of Science and 
      Technology, Wuhan, Hubei Province, China.
FAU - Yuan, Xianglin
AU  - Yuan X
AUID- ORCID: 0000-0003-0201-3828
AD  - Department of Oncology, Tongji Hospital, Huazhong University of Science and 
      Technology, Wuhan, Hubei Province, China.
LA  - eng
PT  - Journal Article
DEP - 20180212
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
RN  - 0 (Biomarkers)
RN  - 0 (MicroRNAs)
RN  - 0 (Snail Family Transcription Factors)
RN  - 0 (mirn199 microRNA, human)
SB  - IM
MH  - Biomarkers/metabolism
MH  - *Cell Differentiation
MH  - Coculture Techniques
MH  - Fibroblasts/*cytology/metabolism
MH  - Fibrosis
MH  - Gene Expression Regulation
MH  - Human Umbilical Vein Endothelial Cells/*metabolism/*radiation effects
MH  - Humans
MH  - Mesoderm/*metabolism
MH  - MicroRNAs/genetics/*metabolism
MH  - Myofibroblasts/*cytology/metabolism
MH  - Signal Transduction/genetics
MH  - Snail Family Transcription Factors/*metabolism
MH  - X-Rays
PMC - PMC5830288
EDAT- 2018/04/06 06:00
MHDA- 2018/09/18 06:00
PMCR- 2018/02/12
CRDT- 2018/04/06 06:00
PHST- 2017/10/27 00:00 [received]
PHST- 2018/01/09 00:00 [revised]
PHST- 2018/01/17 00:00 [accepted]
PHST- 2018/04/06 06:00 [entrez]
PHST- 2018/04/06 06:00 [pubmed]
PHST- 2018/09/18 06:00 [medline]
PHST- 2018/02/12 00:00 [pmc-release]
AID - 10.1155/2018/4135806 [doi]
PST - epublish
SO  - Biomed Res Int. 2018 Feb 12;2018:4135806. doi: 10.1155/2018/4135806. eCollection 
      2018.