PMID- 29620181
OWN - NLM
STAT- MEDLINE
DCOM- 20180926
LR  - 20181114
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 17
IP  - 6
DP  - 2018 Jun
TI  - Identification of Streptococcus mitis321A vaccine antigens based on reverse 
      vaccinology.
PG  - 7477-7486
LID - 10.3892/mmr.2018.8799 [doi]
AB  - Streptococcus mitis (S. mitis) may transform into highly pathogenic bacteria. The 
      aim of the present study was to identify potential antigen targets for designing 
      an effective vaccine against the pathogenic S. mitis321A. The genome of S. 
      mitis321A was sequenced using an Illumina Hiseq2000 instrument. Subsequently, 
      Glimmer 3.02 and Tandem Repeat Finder (TRF) 4.04 were used to predict genes and 
      tandem repeats, respectively, with DNA sequence function analysis using the Basic 
      Local Alignment Search Tool (BLAST) in the Kyoto Encyclopedia of Genes and 
      Genomes (KEGG) and Cluster of Orthologous Groups of proteins (COG) databases. 
      Putative gene antigen candidates were screened with BLAST ahead of phylogenetic 
      tree analysis. The DNA sequence assembly size was 2,110,680 bp with 40.12% GC, 6 
      scaffolds and 9 contig. Consequently, 1,944 genes were predicted, and 119 TRF, 56 
      microsatellite DNA, 10 minisatellite DNA and 154 transposons were acquired. The 
      predicted genes were associated with various pathways and functions concerning 
      membrane transport and energy metabolism. Multiple putative genes encoding 
      surface proteins, secreted proteins and virulence factors, as well as essential 
      genes were determined. The majority of essential genes belonged to a phylogenetic 
      lineage, while 321AGL000129 and 321AGL000299 were on the same branch. The current 
      study provided useful information regarding the biological function of the S. 
      mitis321A genome and recommends putative antigen candidates for developing a 
      potent vaccine against S. mitis.
FAU - Zhang, Qiao
AU  - Zhang Q
AD  - Institute of Respiratory Disease, Xinqiao Hospital of Third Military Medical 
      University, Chongqing 400037, P.R. China.
FAU - Lin, Kexiong
AU  - Lin K
AD  - Institute of Respiratory Disease, Xinqiao Hospital of Third Military Medical 
      University, Chongqing 400037, P.R. China.
FAU - Wang, Changzheng
AU  - Wang C
AD  - Institute of Respiratory Disease, Xinqiao Hospital of Third Military Medical 
      University, Chongqing 400037, P.R. China.
FAU - Xu, Zhi
AU  - Xu Z
AD  - Institute of Respiratory Disease, Xinqiao Hospital of Third Military Medical 
      University, Chongqing 400037, P.R. China.
FAU - Yang, Li
AU  - Yang L
AD  - Institute of Respiratory Disease, Xinqiao Hospital of Third Military Medical 
      University, Chongqing 400037, P.R. China.
FAU - Ma, Qianli
AU  - Ma Q
AD  - Institute of Respiratory Disease, Xinqiao Hospital of Third Military Medical 
      University, Chongqing 400037, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20180328
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Bacterial Vaccines)
SB  - IM
MH  - Antigens, Bacterial/genetics/*immunology
MH  - Bacterial Vaccines/genetics/*immunology
MH  - Computational Biology/methods
MH  - Genome, Bacterial
MH  - Genomics/methods
MH  - Humans
MH  - Immunogenicity, Vaccine
MH  - Molecular Sequence Annotation
MH  - Phylogeny
MH  - Streptococcal Infections/*immunology/microbiology/prevention & control
MH  - Streptococcus/classification/genetics/*immunology
PMC - PMC5983942
EDAT- 2018/04/06 06:00
MHDA- 2018/09/27 06:00
PMCR- 2018/03/28
CRDT- 2018/04/06 06:00
PHST- 2016/06/23 00:00 [received]
PHST- 2017/04/19 00:00 [accepted]
PHST- 2018/04/06 06:00 [pubmed]
PHST- 2018/09/27 06:00 [medline]
PHST- 2018/04/06 06:00 [entrez]
PHST- 2018/03/28 00:00 [pmc-release]
AID - mmr-17-06-7477 [pii]
AID - 10.3892/mmr.2018.8799 [doi]
PST - ppublish
SO  - Mol Med Rep. 2018 Jun;17(6):7477-7486. doi: 10.3892/mmr.2018.8799. Epub 2018 Mar 
      28.