PMID- 29620288
OWN - NLM
STAT- MEDLINE
DCOM- 20180926
LR  - 20181202
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Linking)
VI  - 17
IP  - 6
DP  - 2018 Jun
TI  - Differentiation of genetically modified canine bone mesenchymal stem cells 
      labeled with superparamagnetic iron oxide into neural‑like cells.
PG  - 7902-7910
LID - 10.3892/mmr.2018.8847 [doi]
AB  - The use of mesenchymal stem cells (MSCs) has been reported to improve outcomes in 
      various types of nervous system diseases, primarily based on their neural 
      regenerative differentiation ability and paracrine effect on different 
      neuroprotective cytokines. Genetically modified MSCs may enhance the paracrine 
      effect and may further improve the cell‑based therapeutic outcome of nervous 
      system diseases. Magnetic resonance imaging has been used to monitor distribution 
      and migration of cells labeled with superparamagnetic iron oxide (SPIO) 
      nanoparticles. However, few studies have described the neural differentiation 
      ability of genetically modified and SPIO‑labeled MSCs, which is the foundation 
      for cell tracking and cell therapy in vivo. In this study, canine bone 
      marrow‑derived MSCs (BMSCs) were initially labeled with SPIO, by culturing with 
      20 microg/ml SPIO for 24 h, and transfected with the brain‑derived neurotrophic 
      factor (BDNF) gene using lentivirus transfection at different multiplicities of 
      infection (MOI) values. The optimized MOI value was demonstrated by cellular 
      viability and enhanced green fluorescent protein (eGFP) rate. Subsequently, the 
      BMSCs were induced to differentiate into neuron‑like cells by chemical induction. 
      The results demonstrated that BDNF‑overexpressing BMSCs labeled with SPIO can be 
      induced into neuron‑like cells with high efficiency and minimal effects on cell 
      viability. Additionally, following neural differentiation, the cells transfected 
      with BDNF and labeled with SPIO expressed significantly higher levels of BDNF and 
      neural markers. The overexpression of BDNF may contribute to neural 
      differentiation of BDNFs, and may have potential benefits for further BMSC‑based 
      therapy in vivo.
FAU - Liu, Xing-Long
AU  - Liu XL
AD  - Radiology Department, The First Affiliated Hospital of Nanjing Medical 
      University, Jiangsu Province Hospital, Nanjing, Jiangsu 210029, P.R. China.
FAU - Zu, Qing-Quan
AU  - Zu QQ
AD  - Radiology Department, The First Affiliated Hospital of Nanjing Medical 
      University, Jiangsu Province Hospital, Nanjing, Jiangsu 210029, P.R. China.
FAU - Wang, Bin
AU  - Wang B
AD  - Radiology Department, The First Affiliated Hospital of Nanjing Medical 
      University, Jiangsu Province Hospital, Nanjing, Jiangsu 210029, P.R. China.
FAU - Lu, Shan-Shan
AU  - Lu SS
AD  - Radiology Department, The First Affiliated Hospital of Nanjing Medical 
      University, Jiangsu Province Hospital, Nanjing, Jiangsu 210029, P.R. China.
FAU - Xu, Xiao-Quan
AU  - Xu XQ
AD  - Radiology Department, The First Affiliated Hospital of Nanjing Medical 
      University, Jiangsu Province Hospital, Nanjing, Jiangsu 210029, P.R. China.
FAU - Liu, Sheng
AU  - Liu S
AD  - Radiology Department, The First Affiliated Hospital of Nanjing Medical 
      University, Jiangsu Province Hospital, Nanjing, Jiangsu 210029, P.R. China.
FAU - Shi, Hai-Bin
AU  - Shi HB
AD  - Radiology Department, The First Affiliated Hospital of Nanjing Medical 
      University, Jiangsu Province Hospital, Nanjing, Jiangsu 210029, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20180405
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Biomarkers)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Ferric Compounds)
RN  - 0 (Magnetite Nanoparticles)
RN  - 1K09F3G675 (ferric oxide)
SB  - IM
MH  - Animals
MH  - Biomarkers
MH  - Brain-Derived Neurotrophic Factor/genetics/metabolism
MH  - *Cell Differentiation/genetics
MH  - Dogs
MH  - Female
MH  - Ferric Compounds
MH  - Magnetite Nanoparticles
MH  - Male
MH  - Mesenchymal Stem Cells/*cytology/*metabolism
MH  - Phenotype
EDAT- 2018/04/06 06:00
MHDA- 2018/09/27 06:00
CRDT- 2018/04/06 06:00
PHST- 2017/08/28 00:00 [received]
PHST- 2017/12/19 00:00 [accepted]
PHST- 2018/04/06 06:00 [pubmed]
PHST- 2018/09/27 06:00 [medline]
PHST- 2018/04/06 06:00 [entrez]
AID - 10.3892/mmr.2018.8847 [doi]
PST - ppublish
SO  - Mol Med Rep. 2018 Jun;17(6):7902-7910. doi: 10.3892/mmr.2018.8847. Epub 2018 Apr 
      5.