PMID- 29620512
OWN - NLM
STAT- MEDLINE
DCOM- 20190819
LR  - 20190819
IS  - 1001-9294 (Print)
IS  - 1001-9294 (Linking)
VI  - 33
IP  - 1
DP  - 2018 Mar 30
TI  - Astragaloside Ⅳ Protects Against Abeta1-42-induced Oxidative Stress, 
      Neuroinflammation and Cognitive Impairment in Rats.
PG  - 29-37
LID - 10.24920/11802 [doi]
AB  - Objective To investigate the neuroprotective action of astragaloside Ⅳ (AS-Ⅳ) on 
      spatial learning and memory impairment induced by amyloid-beta 1-42 (Abeta1-42) in 
      rats and elucidate its underlying molecular mechanisms. Methods Adult-male 
      Sprague-Dawley rats (230-250 g) were divided into six groups randomly: control, 
      Abeta1-42, AS-Ⅳ, Abeta1-42 plus 5 mg/kg.d AS-Ⅳ, Abeta1-42 plus 25 mg/kg.d AS-Ⅳ, and Abeta1-42 
      plus 50 mg/kg.d AS-Ⅳ groups. Abeta1-42 were delivered by intracerebroventricular 
      injection under the guidance of a brain stereotaxic apparatus. The Morris water 
      maze test (hidden platform test, probe trials, visible platform test) was 
      performed one week after Abeta1-42 injection to obtain the ability of rat spatial 
      learning and memory. AS-Ⅳ (5, 25 and 50 mg/kg.d) was administrated 
      intraperitoneally once per day from the 8th day after Abeta1-42 injection for 5 
      consecutive days. Average escape latencies, distances for searching for the 
      platform under water and the percentage of total time elapsed and distance swam 
      in the right quadrant after removing platform were determined by behavior 
      software system. The vision and swim speeds of rats were also determined to 
      exclude the effect of these factors on the parameters of learning and memory. 
      After behavioral tests, the rats were sacrificed immediately by decapitation. 
      Hippocampus were collected. The enzyme activities of superoxide dismutase (SOD), 
      glutathione peroxidase (GSH-px) and catalase (CAT) in the hippocampus obtained 
      from different-treated rat brain were measured by following the manufacturer's 
      instructions. The levels of interleukin-1 beta (IL-1beta) and tumor necrosis 
      factor-alpha (TNF-alpha) in tissue lysates were assayed with ELISA. Results The water 
      maze test results indicated that chronic treatments with AS-Ⅳ effectively 
      protected the rats from Abeta1-42-induced spatial learning and memory impairment. 
      Furthermore, the activities of SOD, GSH-px and CAT decreased by Abeta1-42 were also 
      restored by AS-Ⅳ treatment in the hippocampus of rats. In addition, AS-Ⅳ 
      significantly decreased the levels of IL-1beta and TNF-alpha in the hippocampus of 
      Abeta1-42-induced amnesia's rats. Conclusion Our findings suggest that AS-Ⅳ might be 
      a useful chemical in improving the spatial memory and relieving the oxidative 
      stress and neuroinflammation in Alzheimer patients.
FAU - Pan, Yan-Fang
AU  - Pan YF
AD  - Department of Pathology, Shaanxi University of Chinese Medicine, Xianyang, 
      Shaanxi 712046, China.
FAU - Jia, Xiao-Tao
AU  - Jia XT
AD  - Department of Neurology, The Affiliated Xi'an Central Hospital of Xi'an Jiaotong 
      University College of Medicine, Xi'an 710003, China;
FAU - Song, Er-Fei
AU  - Song EF
AD  - Department of Biology, York University, Toronto, ON M3J 1P3, Canada.
FAU - Peng, Xiao-Zhong
AU  - Peng XZ
AD  - State Key Laboratory of Medical Molecular Biology, Department of Molecular 
      Biology and Biochemistry, Institute of Basic Medical Sciences Chinese Academy of 
      Medical Sciences & School of Basic Medicine Peking Union Medical College, Beijing 
      100005, China.
LA  - eng
PT  - Journal Article
PL  - China
TA  - Chin Med Sci J
JT  - Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih
JID - 9112559
RN  - 0 (Interleukin-1beta)
RN  - 0 (Saponins)
RN  - 0 (Triterpenes)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 3A592W8XKE (astragaloside A)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
MH  - Animals
MH  - Catalase/metabolism
MH  - Cognitive Dysfunction/*prevention & control
MH  - Interleukin-1beta/metabolism
MH  - Male
MH  - Maze Learning/drug effects
MH  - Memory/drug effects
MH  - Memory Disorders/*prevention & control
MH  - Oxidative Stress/*drug effects
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Saponins/*therapeutic use
MH  - Spatial Learning/drug effects
MH  - Superoxide Dismutase/metabolism
MH  - Triterpenes/*therapeutic use
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2018/04/06 06:00
MHDA- 2019/08/20 06:00
CRDT- 2018/04/06 06:00
PHST- 2018/04/06 06:00 [entrez]
PHST- 2018/04/06 06:00 [pubmed]
PHST- 2019/08/20 06:00 [medline]
AID - 10.24920/11802 [doi]
PST - ppublish
SO  - Chin Med Sci J. 2018 Mar 30;33(1):29-37. doi: 10.24920/11802.