PMID- 29622212 OWN - NLM STAT- MEDLINE DCOM- 20180917 LR - 20181202 IS - 1873-7072 (Electronic) IS - 0308-8146 (Linking) VI - 257 DP - 2018 Aug 15 TI - Development of an upconversion fluorescence DNA probe for the detection of acetamiprid by magnetic nanoparticles separation. PG - 289-294 LID - S0308-8146(18)30396-0 [pii] LID - 10.1016/j.foodchem.2018.02.148 [doi] AB - An upconversion fluorescence DNA probe which consists of aptamer-conjugated magnet nanoparticles (apt-MNPs) and complementary DNA-conjugated upconversion nanoparticles (cDNA-UCNPs) was developed to detect acetamiprid. Acetamiprid can specifically conjugate with the apt-MNPs to dissociate the cDNA-UCNPs from the apt-MNPs and resulted in reduced fluorescence intensity through an external magnet. The change of fluorescence intensity ( big up tri, openI) is positively related to the concentration of acetamiprid, which can be applied for the quantification of acetamiprid. Under optimal conditions, a linear detection range and detection limit are 0.89-114.18 mug/L and 0.65 mug/L, respectively. The probe was successfully used to detect acetamiprid in spiked paddy water, soil, pear, apple, wheat and cucumber. Average recoveries are 78.2%-103.5% with intra-day relative standard deviations (RSDs) of 2.6%-10.9% and inter-day RSDs of 4.3%-10.2%. The amounts of acetamiprid in the authentic paddy water and pear samples detected by the DNA probe are significantly correlated with that detected by high-performance liquid chromatography (HPLC). CI - Copyright (c) 2018 Elsevier Ltd. All rights reserved. FAU - Sun, Nana AU - Sun N AD - College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing 210095, China. FAU - Ding, Yuan AU - Ding Y AD - College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing 210095, China. FAU - Tao, Zhexuan AU - Tao Z AD - College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing 210095, China. FAU - You, Hongjie AU - You H AD - College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing 210095, China. FAU - Hua, Xiude AU - Hua X AD - College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing 210095, China. FAU - Wang, Minghua AU - Wang M AD - College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing 210095, China. Electronic address: wangmha@njau.edu.cn. LA - eng PT - Journal Article DEP - 20180302 PL - England TA - Food Chem JT - Food chemistry JID - 7702639 RN - 0 (Aptamers, Nucleotide) RN - 0 (DNA Probes) RN - 0 (Fluorescent Dyes) RN - 0 (Insecticides) RN - 0 (Magnetite Nanoparticles) RN - 0 (Neonicotinoids) RN - 0 (Soil Pollutants) RN - 0 (Water Pollutants, Chemical) RN - 5HL5N372P0 (acetamiprid) SB - IM MH - Aptamers, Nucleotide/chemistry MH - Cucumis sativus/chemistry MH - DNA Probes/*chemistry MH - Fluorescent Dyes/*chemistry MH - Food Contamination/*analysis MH - Insecticides/analysis MH - Limit of Detection MH - Magnetite Nanoparticles/*chemistry MH - Malus/chemistry MH - Neonicotinoids/*analysis MH - Pyrus/chemistry MH - Soil Pollutants/analysis MH - Spectroscopy, Fourier Transform Infrared MH - Triticum/chemistry MH - Water Pollutants, Chemical/analysis OTO - NOTNLM OT - Acetamiprid OT - Aptamer OT - DNA probe OT - Magnetic nanoparticles OT - Upconversion nanoparticles EDAT- 2018/04/07 06:00 MHDA- 2018/09/18 06:00 CRDT- 2018/04/07 06:00 PHST- 2017/10/26 00:00 [received] PHST- 2018/02/24 00:00 [revised] PHST- 2018/02/27 00:00 [accepted] PHST- 2018/04/07 06:00 [entrez] PHST- 2018/04/07 06:00 [pubmed] PHST- 2018/09/18 06:00 [medline] AID - S0308-8146(18)30396-0 [pii] AID - 10.1016/j.foodchem.2018.02.148 [doi] PST - ppublish SO - Food Chem. 2018 Aug 15;257:289-294. doi: 10.1016/j.foodchem.2018.02.148. Epub 2018 Mar 2.