PMID- 29624001
OWN - NLM
STAT- MEDLINE
DCOM- 20190809
LR  - 20190809
IS  - 1505-1773 (Print)
IS  - 1505-1773 (Linking)
VI  - 21
IP  - 1
DP  - 2018 Mar
TI  - Magnolol inhibits Streptococcus suis-induced inflammation and ROS formation via 
      TLR2/MAPK/NF-kappaB signaling in RAW264.7 cells.
PG  - 111-118
LID - 10.24425/119028 [doi]
AB  - Our previous studies have shown that Magnolol (Mag) improves the symptoms and 
      decreases the levels of cytokines during infection induced by Streptococcus suis 
      (S. suis) in mice. Although some reports show that Mag inhibits 
      lipopolysaccharide-induced inflammatory responses via downregulating 
      mitogen-activated protein kinases (MAPK) and nuclear factor-kappaB (NF-kappaB) signaling 
      pathways, the molecular mechanisms underlying Mag-mediated inhibition of S. 
      suis-induced inflammatory responses are poorly understood. Here, RAW264.7 cells 
      were stimulated with S. suis in the presence or absence of Mag. Cell viability 
      and bactericidal effects were examined, and the concentrations of tumor necrosis 
      factor-a (TNF-alpha), IL-1beta (interleukin-1beta), IL-6 (interleukin-6), and IL-8 
      (interleukin- 8) were determined by ELISA. The change in ROS (reactive oxygen 
      species) was determined by fluorescence microscopy and ELISA. The levels of 
      Toll-like receptor 2 (TLR2) and MAPK family proteins and NF-kappaB signaling were 
      determined by Western blot analysis. S. suis induced massive RAW264.7 cell death, 
      a decline in bactericidal activity, the release of inflammatory cytokines, 
      increased oxidative stress, and activation of TLR2/MAPK/NF-kappaB signaling pathways. 
      Mag treatment significantly suppressed macrophage cell death and caused a decline 
      in bactericidal activity. Furthermore, Mag decreased inflammatory cytokines 
      production and ROS generation. It also prevented p38, extracellular regulated 
      protein kinases (ERK), c-Jun N-terminal kinase (JNK), inhibitor of NF-kappaB (IkappaB), 
      and NF-kappaB phosphorylation induced by S. suis in a dose-dependent manner. Our 
      results indicate that Mag exerts anti-inflammatory and cell-protective effects 
      and mediates the activation of MAPK and NF-kappaB signaling by downregulating the 
      expression of TLR2 upregulated by S. suis.
CI  - Copyright(c) by the Polish Academy of Sciences.
FAU - Zhang, L
AU  - Zhang L
AD  - Institute of Animal Husbandry and Veterinary Medicine, Jinzhou Medical 
      University, 48 the People Street, Guta District, Jinzhou, Liaoning 121001, 
      People's Republic of China.
FAU - Wang, J
AU  - Wang J
AD  - Institute of Animal Husbandry and Veterinary Medicine, Jinzhou Medical 
      University, 48 the People Street, Guta District, Jinzhou, Liaoning 121001, 
      People's Republic of China.
FAU - Xu, W
AU  - Xu W
AD  - First Affiliated Hospital, Jinzhou Medical University, 48 the People Street, Guta 
      District, Jinzhou, Liaoning 121001, People's Republic of China.
FAU - Sun, Y
AU  - Sun Y
AD  - First Affiliated Hospital, Jinzhou Medical University, 48 the People Street, Guta 
      District, Jinzhou, Liaoning 121001, People's Republic of China.
FAU - You, J
AU  - You J
AD  - Institute of Animal Husbandry and Veterinary Medicine, Jinzhou Medical 
      University, 48 the People Street, Guta District, Jinzhou, Liaoning 121001, 
      People's Republic of China.
FAU - Lu, H
AU  - Lu H
AD  - Institute of Animal Husbandry and Veterinary Medicine, Jinzhou Medical 
      University, 48 the People Street, Guta District, Jinzhou, Liaoning 121001, 
      People's Republic of China.
FAU - Song, Y
AU  - Song Y
AD  - Institute of Animal Husbandry and Veterinary Medicine, Jinzhou Medical 
      University, 48 the People Street, Guta District, Jinzhou, Liaoning 121001, 
      People's Republic of China.
FAU - Wei, J
AU  - Wei J
AD  - Institute of Animal Husbandry and Veterinary Medicine, Jinzhou Medical 
      University, 48 the People Street, Guta District, Jinzhou, Liaoning 121001, 
      People's Republic of China.
FAU - Li, L
AU  - Li L
AD  - Institute of Animal Husbandry and Veterinary Medicine, Jinzhou Medical 
      University, 48 the People Street, Guta District, Jinzhou, Liaoning 121001, 
      People's Republic of China.
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Pol J Vet Sci
JT  - Polish journal of veterinary sciences
JID - 101125473
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Biphenyl Compounds)
RN  - 0 (Lignans)
RN  - 0 (NF-kappa B)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Tlr2 protein, mouse)
RN  - 0 (Toll-Like Receptor 2)
RN  - 001E35HGVF (magnolol)
RN  - EC 2.7.12.2 (Mitogen-Activated Protein Kinase Kinases)
MH  - Animals
MH  - Anti-Bacterial Agents/pharmacology
MH  - Biphenyl Compounds/chemistry/*pharmacology
MH  - Gene Expression Regulation/drug effects
MH  - Lignans/chemistry/*pharmacology
MH  - Mice
MH  - Mitogen-Activated Protein Kinase Kinases/genetics/*metabolism
MH  - Molecular Structure
MH  - NF-kappa B/genetics/*metabolism
MH  - RAW 264.7 Cells
MH  - Reactive Oxygen Species
MH  - Streptococcal Infections
MH  - Streptococcus suis
MH  - Toll-Like Receptor 2/genetics/*metabolism
OTO - NOTNLM
OT  - Streptococcus suis
OT  - Toll-like receptor2
OT  - magnolol
OT  - mitogen-activated protein kinase
OT  - nuclear factor Kappa B
EDAT- 2018/04/07 06:00
MHDA- 2019/08/10 06:00
CRDT- 2018/04/07 06:00
PHST- 2018/04/07 06:00 [entrez]
PHST- 2018/04/07 06:00 [pubmed]
PHST- 2019/08/10 06:00 [medline]
AID - 10.24425/119028 [doi]
PST - ppublish
SO  - Pol J Vet Sci. 2018 Mar;21(1):111-118. doi: 10.24425/119028.