PMID- 29625078
OWN - NLM
STAT- MEDLINE
DCOM- 20190912
LR  - 20190912
IS  - 1872-7905 (Electronic)
IS  - 0022-1759 (Print)
IS  - 0022-1759 (Linking)
VI  - 457
DP  - 2018 Jun
TI  - Automated generation of immature dendritic cells in a single-use system.
PG  - 53-65
LID - S0022-1759(18)30033-4 [pii]
LID - 10.1016/j.jim.2018.03.010 [doi]
AB  - Dendritic cells (DCs) are an indispensable part of studying human responses that 
      are important for protective immunity against cancer and infectious diseases as 
      well as prevention of autoimmunity and transplant rejection. These cells are also 
      key elements of personalized vaccines for cancer and infectious diseases. Despite 
      the vital role of DCs in both clinical and basic research contexts, methods for 
      obtaining these cells from individuals remains a comparatively under-developed 
      and inefficient process. DCs are present in very low concentrations (<1%) in 
      blood, thus they must be generated from monocytes and the current methodology in 
      DC generation involves a laborious process of static culture and stimulation with 
      cytokines contained in culture medium. Herein, we describe an automated fluidic 
      system, MicroDEN, that allows for differentiation of monocytes into immature-DCs 
      (iDCs) utilizing continuous perfusion of differentiation media. Manual steps 
      associated with current ex vivo monocyte differentiation are vastly reduced and 
      an aseptic environment is ensured by the use of an enclosed cartridge and tubing 
      network. Benchmark phenotyping was performed on the generated iDCs along with 
      allogeneic T-cell proliferation and syngeneic antigen-specific functional assays. 
      MicroDEN generated iDCs were phenotypically and functionally similar to well 
      plate generated iDCs, thereby demonstrating the feasibility of utilizing MicroDEN 
      in the broad range of applications requiring DCs.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Kozbial, Andrew
AU  - Kozbial A
AD  - Northeastern University, Department of Chemical Engineering, Boston, MA 02115, 
      United States.
FAU - Bhandary, Lekhana
AU  - Bhandary L
AD  - Northeastern University, Department of Chemical Engineering, Boston, MA 02115, 
      United States.
FAU - Collier, Bradley B
AU  - Collier BB
AD  - Northeastern University, Department of Chemical Engineering, Boston, MA 02115, 
      United States.
FAU - Eickhoff, Christopher S
AU  - Eickhoff CS
AD  - Saint Louis University, School of Medicine, Department of Internal Medicine, St. 
      Louis, MO 63104, United States.
FAU - Hoft, Daniel F
AU  - Hoft DF
AD  - Saint Louis University, School of Medicine, Department of Internal Medicine, St. 
      Louis, MO 63104, United States; Saint Louis University, School of Medicine, 
      Department of Molecular Microbiology & Immunology, St. Louis, MO 63104, United 
      States.
FAU - Murthy, Shashi K
AU  - Murthy SK
AD  - Northeastern University, Department of Chemical Engineering, Boston, MA 02115, 
      United States. Electronic address: s.murthy@northeastern.edu.
LA  - eng
GR  - U24 AI118665/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20180403
PL  - Netherlands
TA  - J Immunol Methods
JT  - Journal of immunological methods
JID - 1305440
SB  - IM
MH  - Antigen-Presenting Cells/cytology
MH  - Automation, Laboratory/*instrumentation/*methods
MH  - *Cell Culture Techniques
MH  - *Cell Differentiation
MH  - Cells, Cultured
MH  - Dendritic Cells/*cytology
MH  - Flow Cytometry
MH  - Humans
MH  - Lymphocyte Activation
MH  - Monocytes/cytology
PMC - PMC5946316
MID - NIHMS960194
OTO - NOTNLM
OT  - Antigen-presenting cell
OT  - Cancer vaccinology
OT  - Dendritic cell
OT  - Monocyte
OT  - Personalized medicine
OT  - Vaccine
EDAT- 2018/04/07 06:00
MHDA- 2019/09/13 06:00
PMCR- 2019/06/01
CRDT- 2018/04/07 06:00
PHST- 2018/01/30 00:00 [received]
PHST- 2018/03/26 00:00 [revised]
PHST- 2018/03/26 00:00 [accepted]
PHST- 2018/04/07 06:00 [pubmed]
PHST- 2019/09/13 06:00 [medline]
PHST- 2018/04/07 06:00 [entrez]
PHST- 2019/06/01 00:00 [pmc-release]
AID - S0022-1759(18)30033-4 [pii]
AID - 10.1016/j.jim.2018.03.010 [doi]
PST - ppublish
SO  - J Immunol Methods. 2018 Jun;457:53-65. doi: 10.1016/j.jim.2018.03.010. Epub 2018 
      Apr 3.