PMID- 29625215
OWN - NLM
STAT- MEDLINE
DCOM- 20190405
LR  - 20220330
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Linking)
VI  - 381
DP  - 2018 Jun 15
TI  - H3K9 Acetylation of Tph2 Involved in Depression-like Behavior in Male, but not 
      Female, Juvenile Offspring Rat Induced by Prenatal Stress.
PG  - 138-148
LID - S0306-4522(18)30241-0 [pii]
LID - 10.1016/j.neuroscience.2018.03.043 [doi]
AB  - Increasing evidence has shown that prenatal stress (PS) could cause 
      depression-like behavior in the offspring, which is sex-specific. However, the 
      underlying mechanisms remain to be elucidated. This study is to investigate the 
      involvement of tryptophan hydroxylase 2 (Tph2) H3K9 acetylation (H3K9ac) 
      modification on PS-induced depression-like behavior in juvenile offspring rats 
      (JOR). PS models were established, with or without trichostatin A (TSA) 
      treatment. Animal behavior was assessed by the sucrose preference test (SPT) and 
      forced swimming test (FST). The mRNA and protein expression levels of TPH2 in the 
      dorsal raphenucleus (DRN), hippocampus, and prefrontal cortex were detected with 
      quantitative real-time PCR and Western blot analysis, respectively. The Tph2 
      H3K9ac levels in the hippocampus were also analyzed. SPT and FST showed 
      significantly reduced sucrose preference and significantly prolonged immobility 
      in PS-induced male juvenile offspring rats (MJOR). Moreover, the mRNA and protein 
      expression levels of TPH2 in the DRN and hippocampus were significantly declined, 
      while the hippocampal Tph2 H3K9ac levels were significantly declined in the 
      PS-induced MJOR. Furthermore, the PS-induced effects in MJOR could be reversed by 
      the microinjection of TSA. However, no significant effects were observed for the 
      female juvenile offspring rats (FJORs). In conclusion, our results showed that 
      the Tph2 H3K9ac modification is only involved in PS-induced depression-like 
      behavior in MJOR, in a sex-specific manner. These findings might contribute to 
      the understanding of the disease pathogenesis and clinical treatment in future.
CI  - Copyright (c) 2018 IBRO. Published by Elsevier Ltd. All rights reserved.
FAU - Dang, Shaokang
AU  - Dang S
AD  - Division of Neonatology, The First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an 710061, Shaanxi, China.
FAU - Lu, Yong
AU  - Lu Y
AD  - Department of Genetics, Xi'an Jiaotong University Health Science Center, Xi'an 
      710061, Shaanxi, China.
FAU - Su, Qian
AU  - Su Q
AD  - Division of Neonatology, The First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an 710061, Shaanxi, China.
FAU - Lin, Tianwei
AU  - Lin T
AD  - Key Laboratory of Shaanxi Province Biomedicine, College of Life Sciences, 
      Northwest University, Xi'an 710069, Shaanxi, China.
FAU - Zhang, Xiaoxiao
AU  - Zhang X
AD  - Key Laboratory of Shaanxi Province Biomedicine, College of Life Sciences, 
      Northwest University, Xi'an 710069, Shaanxi, China.
FAU - Zhang, Huiping
AU  - Zhang H
AD  - Division of Neonatology, The First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an 710061, Shaanxi, China.
FAU - Zhang, Junli
AU  - Zhang J
AD  - Key Laboratory of Shaanxi Province Biomedicine, College of Life Sciences, 
      Northwest University, Xi'an 710069, Shaanxi, China.
FAU - Zhang, Lin
AU  - Zhang L
AD  - Key Laboratory of Shaanxi Province Biomedicine, College of Life Sciences, 
      Northwest University, Xi'an 710069, Shaanxi, China.
FAU - Zhu, Zhongliang
AU  - Zhu Z
AD  - Key Laboratory of Shaanxi Province Biomedicine, College of Life Sciences, 
      Northwest University, Xi'an 710069, Shaanxi, China. Electronic address: 
      zlzhu@mail.xjtu.edu.cn.
FAU - Li, Hui
AU  - Li H
AD  - Division of Neonatology, The First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an 710061, Shaanxi, China. Electronic address: 
      huili@mail.xjtu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180404
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Histones)
RN  - EC 1.14.16.4 (Tryptophan Hydroxylase)
RN  - EC 1.14.16.4 (tph2 protein, rat)
SB  - IM
MH  - Acetylation
MH  - Animals
MH  - Behavior, Animal
MH  - Brain/metabolism
MH  - Depression/*etiology/metabolism
MH  - Female
MH  - Gene Expression Regulation, Developmental
MH  - Histones/*metabolism
MH  - Male
MH  - Pregnancy
MH  - *Prenatal Exposure Delayed Effects/metabolism/psychology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Sex Characteristics
MH  - Stress, Psychological/*complications/metabolism
MH  - Tryptophan Hydroxylase/*metabolism
OTO - NOTNLM
OT  - H3K9 acetylation
OT  - Tph2
OT  - depression-like behavior
OT  - juvenile offspring rats
OT  - prenatal stress
EDAT- 2018/04/07 06:00
MHDA- 2019/04/06 06:00
CRDT- 2018/04/07 06:00
PHST- 2017/10/24 00:00 [received]
PHST- 2018/03/23 00:00 [revised]
PHST- 2018/03/26 00:00 [accepted]
PHST- 2018/04/07 06:00 [pubmed]
PHST- 2019/04/06 06:00 [medline]
PHST- 2018/04/07 06:00 [entrez]
AID - S0306-4522(18)30241-0 [pii]
AID - 10.1016/j.neuroscience.2018.03.043 [doi]
PST - ppublish
SO  - Neuroscience. 2018 Jun 15;381:138-148. doi: 10.1016/j.neuroscience.2018.03.043. 
      Epub 2018 Apr 4.