PMID- 29626380
OWN - NLM
STAT- MEDLINE
DCOM- 20190925
LR  - 20190925
IS  - 1462-2920 (Electronic)
IS  - 1462-2912 (Linking)
VI  - 20
IP  - 5
DP  - 2018 May
TI  - The arbuscular mycorrhizal fungus Rhizophagus irregularis uses a reductive iron 
      assimilation pathway for high-affinity iron uptake.
PG  - 1857-1872
LID - 10.1111/1462-2920.14121 [doi]
AB  - Arbuscular mycorrhizal (AM) fungi can improve iron (Fe) acquisition of their host 
      plants. Here, we report a characterization of two components of the high-affinity 
      reductive Fe uptake system of Rhizophagus irregularis, the ferric reductase 
      (RiFRE1) and the high affinity Fe permeases (RiFTR1-2). In the extraradical 
      mycelia (ERM), Fe deficiency induced activation of a plasma membrane-localized 
      ferric reductase, an enzyme that reduces Fe(III) sources to the more soluble 
      Fe(II). Yeast mutant complementation assays showed that RiFRE1 encodes a 
      functional ferric reductase and RiFTR1 an iron permease. In the heterologous 
      system, RiFTR1 was expressed in the plasma membrane while RiFTR2 was expressed in 
      the endomembranes. In the ERM, the highest expression levels of RiFTR1 were found 
      in mycelia grown in media with 0.045 mM Fe, while RiFTR2 was upregulated under 
      Fe-deficient conditions. RiFTR2 expression also increased in the intraradical 
      mycelia (IRM) of maize plants grown without Fe. These data indicate that the Fe 
      permease RiFTR1 plays a key role in Fe acquisition and that RiFTR2 is involved in 
      Fe homeostasis under Fe-limiting conditions. RiFTR1 was highly expressed in the 
      (IRM), which suggests that the maintenance of Fe homeostasis in the IRM might be 
      essential for a successful symbiosis.
CI  - (c) 2018 Society for Applied Microbiology and John Wiley & Sons Ltd.
FAU - Tamayo, Elisabeth
AU  - Tamayo E
AD  - Departamento de Microbiologia del Suelo y Sistemas Simbioticos, Estacion 
      Experimental del Zaidin, CSIC, Granada, Spain.
FAU - Knight, Simon A B
AU  - Knight SAB
AD  - Department of Medicine, Division of Hematology-Oncology, Perelman School of 
      Medicine, University of Pennsylvania, Philadelphia, PA, USA.
FAU - Valderas, Ascension
AU  - Valderas A
AD  - Departamento de Microbiologia del Suelo y Sistemas Simbioticos, Estacion 
      Experimental del Zaidin, CSIC, Granada, Spain.
FAU - Dancis, Andrew
AU  - Dancis A
AD  - Department of Medicine, Division of Hematology-Oncology, Perelman School of 
      Medicine, University of Pennsylvania, Philadelphia, PA, USA.
FAU - Ferrol, Nuria
AU  - Ferrol N
AUID- ORCID: 0000-0001-7963-3537
AD  - Departamento de Microbiologia del Suelo y Sistemas Simbioticos, Estacion 
      Experimental del Zaidin, CSIC, Granada, Spain.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180423
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (Ferric Compounds)
RN  - E1UOL152H7 (Iron)
SB  - IM
MH  - Biological Transport
MH  - Ferric Compounds/metabolism
MH  - Gene Expression Regulation, Fungal
MH  - Glomeromycota/*metabolism
MH  - Homeostasis
MH  - Iron/*metabolism
MH  - Mycelium
MH  - Mycorrhizae/*metabolism
MH  - Saccharomyces cerevisiae/metabolism
MH  - Symbiosis
EDAT- 2018/04/08 06:00
MHDA- 2019/09/26 06:00
CRDT- 2018/04/08 06:00
PHST- 2018/01/10 00:00 [received]
PHST- 2018/03/26 00:00 [accepted]
PHST- 2018/04/08 06:00 [pubmed]
PHST- 2019/09/26 06:00 [medline]
PHST- 2018/04/08 06:00 [entrez]
AID - 10.1111/1462-2920.14121 [doi]
PST - ppublish
SO  - Environ Microbiol. 2018 May;20(5):1857-1872. doi: 10.1111/1462-2920.14121. Epub 
      2018 Apr 23.